Cryo-preservation or cryo-conservation is a process where organelles, cells, tissues, extracellular matrix, organs, or any other biological constructs susceptible to damage caused by unregulated chemical kinetics are preserved by cooling to very low temperatures (typically −80 °C using solid carbon dioxide or −196 °C using liquid nitrogen). At low enough temperatures, any enzymatic or chemical activity which might cause damage to the biological material in question is effectively stopped. Cryopreservation methods seek to reach low temperatures without causing additional damage caused by the formation of ice crystals during freezing. Traditional cryopreservation has relied on coating the material to be frozen with a class of molecules termed cryoprotectants. New methods are constantly being investigated due to the inherent toxicity of many cryoprotectants. By default it should be considered that cryopreservation alters or compromises the structure and function of cells unless it is proven otherwise for a particular cell population. Cryoconservation of animal genetic resources is the process in which animal genetic material is collected and stored with the intention of conservation of the breed.
- 1 Natural cryopreservation
- 2 History
- 3 Temperature
- 4 Risks
- 5 Main methods to prevent risks
- 6 Freezable tissues
- 7 Preservation of microbiology cultures
- 8 See also
- 9 References
- 10 Further reading
Water-bears (Tardigrada), microscopic multicellular organisms, can survive freezing by replacing most of their internal water with the sugar trehalose, preventing it from crystallization that otherwise damages cell membranes. Mixtures of solutes can achieve similar effects. Some solutes, including salts, have the disadvantage that they may be toxic at intense concentrations. In addition to the water-bear, wood frogs can tolerate the freezing of their blood and other tissues. Urea is accumulated in tissues in preparation for overwintering, and liver glycogen is converted in large quantities to glucose in response to internal ice formation. Both urea and glucose act as "cryoprotectants" to limit the amount of ice that forms and to reduce osmotic shrinkage of cells. Frogs can survive many freeze/thaw events during winter if no more than about 65% of the total body water freezes. Research exploring the phenomenon of "freezing frogs" has been performed primarily by the Canadian researcher, Dr. Kenneth B. Storey.
Freeze tolerance, in which organisms survive the winter by freezing solid and ceasing life functions, is known in a few vertebrates: five species of frogs (Rana sylvatica, Pseudacris triseriata, Hyla crucifer, Hyla versicolor, Hyla chrysoscelis), one of salamanders (Hynobius keyserlingi), one of snakes (Thamnophis sirtalis) and three of turtles (Chrysemys picta, Terrapene carolina, Terrapene ornata). Snapping turtles Chelydra serpentina and wall lizards Podarcis muralis also survive nominal freezing but it has not been established to be adaptive for overwintering. In the case of Rana sylvatica one cryopreservant is ordinary glucose, which increases in concentration by approximately 19 mmol/l when the frogs are cooled slowly.
One of the most important early theoreticians of cryopreservation was James Lovelock. In 1953, he suggested that damage to red blood cells during freezing was due to osmotic stress, and that increasing the salt concentration in a dehydrating cell might damage it. In the mid-1950s, he experimented with the cryopreservation of rodents, determining that hamsters could be frozen with 60% of the water in the brain crystallized into ice with no adverse effects; other organs were shown to be susceptible to damage. This work led other scientists to attempt the short-term freezing of rats by 1955, which were fully active 4 to 7 days after being revived.
Cryopreservation was applied to humans beginning in 1954 with three pregnancies resulting from the insemination of previously frozen sperm. Fowl sperm was cryopreserved in 1957 by a team of scientists in the UK directed by Christopher Polge. During 1963, Peter Mazur, at Oak Ridge National Laboratory in the U.S., demonstrated that lethal intracellular freezing could be avoided if cooling was slow enough to permit sufficient water to leave the cell during progressive freezing of the extracellular fluid. That rate differs between cells of differing size and water permeability: a typical cooling rate around 1 °C/minute is appropriate for many mammalian cells after treatment with cryoprotectants such as glycerol or dimethyl sulphoxide, but the rate is not a universal optimum.
The first human body to be frozen with the hope of future revival was James Bedford's, a few hours after his cancer-caused death in 1967. Bedford is the only cryonics patient frozen before 1974 still preserved today.
Storage at very low temperatures is presumed to provide an indefinite longevity to cells, although the actual effective life is rather difficult to prove. Researchers experimenting with dried seeds found that there was noticeable variability of deterioration when samples were kept at different temperatures – even ultra-cold temperatures. Temperatures less than the glass transition point (Tg) of polyol's water solutions, around −136 °C (137 K; −213 °F), seem to be accepted as the range where biological activity very substantially slows, and −196 °C (77 K; −321 °F), the boiling point of liquid nitrogen, is the preferred temperature for storing important specimens. While refrigerators, freezers and extra-cold freezers are used for many items, generally the ultra-cold of liquid nitrogen is required for successful preservation of the more complex biological structures to virtually stop all biological activity.
Phenomena which can cause damage to cells during cryopreservation mainly occur during the freezing stage, and include: solution effects, extracellular ice formation, dehydration and intracellular ice formation. Many of these effects can be reduced by cryoprotectants. Once the preserved material has become frozen, it is relatively safe from further damage. However, estimates based on the accumulation of radiation-induced DNA damage during cryonic storage have suggested a maximum storage period of 1000 years.
- Solution effects
- As ice crystals grow in freezing water, solutes are excluded, causing them to become concentrated in the remaining liquid water. High concentrations of some solutes can be very damaging.
- Extracellular ice formation
- When tissues are cooled slowly, water migrates out of cells and ice forms in the extracellular space. Too much extracellular ice can cause mechanical damage to the cell membrane due to crushing.
- Migration of water, causing extracellular ice formation, can also cause cellular dehydration. The associated stresses on the cell can cause damage directly.
- Intracellular ice formation
- While some organisms and tissues can tolerate some extracellular ice, any appreciable intracellular ice is almost always fatal to cells.
Main methods to prevent risksEdit
The main techniques to prevent cryopreservation damages are a well established combination of controlled rate and slow freezing and a newer flash-freezing process known as vitrification.
Slow programmable freezingEdit
Controlled-rate and slow freezing, also known as slow programmable freezing (SPF), is a set of well established techniques developed during the early 1970s which enabled the first human embryo frozen birth Zoe Leyland during 1984. Since then, machines that freeze biological samples using programmable sequences, or controlled rates, have been used all over the world for human, animal and cell biology – "freezing down" a sample to better preserve it for eventual thawing, before it is frozen, or cryopreserved, in liquid nitrogen. Such machines are used for freezing oocytes, skin, blood products, embryo, sperm, stem cells and general tissue preservation in hospitals, veterinary practices and research laboratories around the world. As an example, the number of live births from frozen embryos 'slow frozen' is estimated at some 300,000 to 400,000 or 20% of the estimated 3 million in vitro fertilisation (IVF) births.
Lethal intracellular freezing can be avoided if cooling is slow enough to permit sufficient water to leave the cell during progressive freezing of the extracellular fluid. To minimize the growth of extracellular ice crystal growth and recrystallization, biomaterials such as alginates, polyvinyl alcohol or chitosan can be used to impede ice crystal growth along with traditional small molecule cryoprotectants. That rate differs between cells of differing size and water permeability: a typical cooling rate of about 1 °C/minute is appropriate for many mammalian cells after treatment with cryoprotectants such as glycerol or dimethyl sulfoxide, but the rate is not a universal optimum. The 1 °C / minute rate can be achieved by using devices such as a rate-controlled freezer or a benchtop portable freezing container.
Several independent studies have provided evidence that frozen embryos stored using slow-freezing techniques may in some ways be 'better' than fresh in IVF. The studies indicate that using frozen embryos and eggs rather than fresh embryos and eggs reduced the risk of stillbirth and premature delivery though the exact reasons are still being explored.
Researchers Greg Fahy and William F. Rall helped to introduce vitrification to reproductive cryopreservation in the mid-1980s. As of 2000, researchers claim vitrification provides the benefits of cryopreservation without damage due to ice crystal formation. The situation became more complex with the development of tissue engineering as both cells and biomaterials need to remain ice-free to preserve high cell viability and functions, integrity of constructs and structure of biomaterials. Vitrification of tissue engineered constructs was first reported by Lilia Kuleshova, who also was the first scientist to achieve vitrification of oocytes, which resulted in live birth in 1999. For clinical cryopreservation, vitrification usually requires the addition of cryoprotectants prior to cooling. Cryoprotectants are macromolecules added to the freezing medium to protect cells from the detrimental effects of intracellular ice crystal formation or from the solution effects, during the process of freezing and thawing. They permit a higher degree of cell survival during freezing, to lower the freezing point, to protect cell membrane from freeze-related injury. Crioprotectants have high solubility, low toxicity at high concentrations, low molecular weight and the ability to interact with water via hydrogen bonding.
Instead of crystallizing, the syrupy solution becomes an amorphous ice—it vitrifies. Rather than a phase change from liquid to solid by crystallization, the amorphous state is like a "solid liquid", and the transformation is over a small temperature range described as the "glass transition" temperature.
Vitrification of water is promoted by rapid cooling, and can be achieved without cryoprotectants by an extremely rapid decrease of temperature (megakelvins per second). The rate that is required to attain glassy state in pure water was considered to be impossible until 2005.
Two conditions usually required to allow vitrification are an increase of the viscosity and a decrease of the freezing temperature. Many solutes do both, but larger molecules generally have a larger effect, particularly on viscosity. Rapid cooling also promotes vitrification.
For established methods of cryopreservation, the solute must penetrate the cell membrane in order to achieve increased viscosity and decrease freezing temperature inside the cell. Sugars do not readily permeate through the membrane. Those solutes that do, such as dimethyl sulfoxide, a common cryoprotectant, are often toxic in intense concentration. One of the difficult compromises of vitrifying cryopreservation concerns limiting the damage produced by the cryoprotectant itself due to cryoprotectant toxicity. Mixtures of cryoprotectants and the use of ice blockers have enabled the Twenty-First Century Medicine company to vitrify a rabbit kidney to −135 °C with their proprietary vitrification mixture. Upon rewarming, the kidney was transplanted successfully into a rabbit, with complete functionality and viability, able to sustain the rabbit indefinitely as the sole functioning kidney.
Blood can be replaced with inert noble gases and/or metabolically vital gases like oxygen, so that organs can cool more quickly and less antifreeze is needed. Since regions of tissue are separated by gas, small expansions do not accumulate, thereby protecting against shattering. A small company, Arigos Biomedical, "has already recovered pig hearts from the 120 degrees below zero", although the definition of "recovered" is not clear. Pressures of 60 atm can help increase heat exchange rates. Gaseous oxygen perfusion/persufflation can enhance organ preservation relative to static cold storage or hypothermic machine perfusion, since the lower viscosity of gases, may help reach more regions of preserved organs and deliver more oxygen per gram tissue.
Generally, cryopreservation is easier for thin samples and suspended cells, because these can be cooled more quickly and so require lesser doses of toxic cryoprotectants. Therefore, cryopreservation of human livers and hearts for storage and transplant is still impractical.
Nevertheless, suitable combinations of cryoprotectants and regimes of cooling and rinsing during warming often allow the successful cryopreservation of biological materials, particularly cell suspensions or thin tissue samples. Examples include:
- Semen in semen cryopreservation
- Tissue samples like tumors and histological cross sections
- Eggs (oocytes) in oocyte cryopreservation
- Embryos at cleavage stage (that are 2, 4 or 8 cells) or at blastocyst stage, in embryo cryopreservation
- Ovarian tissue in ovarian tissue cryopreservation
- Plant seeds or shoots may be cryopreserved for conservation purposes.
Additionally, efforts are underway to preserve humans cryogenically, known as cryonics. For such efforts either the brain within the head or the entire body may experience the above process. Cryonics is in a different category from the aforementioned examples, however: while countless cryopreserved cells, vaccines, tissue and other biological samples have been thawed and used successfully, this has not yet been the case at all for cryopreserved brains or bodies. At issue are the criteria for defining "success".
Proponents of cryonics claim that cryopreservation using present technology, particularly vitrification of the brain, may be sufficient to preserve people in an "information theoretic" sense so that they could be revived and made whole by hypothetical vastly advanced future technology. Not only is there no guarantee of its success, many people[who?] argue that human cryopreservation is unethical. According to certain views[which?] of the mind body problem, some philosophers[who?] believe that the mind, which contains thoughts, memories, and personality, is separate from the brain. When someone dies, their mind leaves the body. If a cryopreserved patient gets successfully resuscitated, no one knows if they would be the same person that they once were or if they would be an empty shell of the memory of who they once were.[improper synthesis?]
Right now scientists are trying to see if transplanting cryopreserved human organs for transplantation is viable, if so this would be a major step forward for the possibility of reviving a cryopreserved human.
Cryopreservation for embryos is used for embryo storage, e.g., when in vitro fertilization (IVF) has resulted in more embryos than is currently needed.
Pregnancies have been reported from embryos stored for 16 years. Many studies have evaluated the children born from frozen embryos, or “frosties”. The result has been uniformly positive with no increase in birth defects or development abnormalities. A study of more than 11,000 cryopreserved human embryos showed no significant effect of storage time on post-thaw survival for IVF or oocyte donation cycles, or for embryos frozen at the pronuclear or cleavage stages. Additionally, the duration of storage did not have any significant effect on clinical pregnancy, miscarriage, implantation, or live birth rate, whether from IVF or oocyte donation cycles. Rather, oocyte age, survival proportion, and number of transferred embryos are predictors of pregnancy outcome.
Cryopreservation of ovarian tissue is of interest to women who want to preserve their reproductive function beyond the natural limit, or whose reproductive potential is threatened by cancer therapy, for example in hematologic malignancies or breast cancer. The procedure is to take a part of the ovary and perform slow freezing before storing it in liquid nitrogen whilst therapy is undertaken. Tissue can then be thawed and implanted near the fallopian, either orthotopic (on the natural location) or heterotopic (on the abdominal wall), where it starts to produce new eggs, allowing normal conception to occur. The ovarian tissue may also be transplanted into mice that are immunocompromised (SCID mice) to avoid graft rejection, and tissue can be harvested later when mature follicles have developed.
Human oocyte cryopreservation is a new technology in which a woman’s eggs (oocytes) are extracted, frozen and stored. Later, when she is ready to become pregnant, the eggs can be thawed, fertilized, and transferred to the uterus as embryos. Since 1999, when the birth of the first baby from an embryo derived from vitrified-warmed woman’s eggs was reported by Kuleshova and co-workers in the journal of Human Reproduction, this concept has been recognized and widespread. This break-through in achieving vitrification of woman’s oocytes made an important advance in our knowledge and practice of the IVF process, as clinical pregnancy rate is four times higher after oocyte vitrification than after slow freezing. Oocyte vitrification is vital for preservation fertility in young oncology patients and for individuals undergoing IVF who object, either for religious or ethical reasons, to the practice of freezing embryos.
Semen can be used successfully almost indefinitely after cryopreservation. The longest reported successful storage is 22 years. It can be used for sperm donation where the recipient wants the treatment in a different time or place, or as a means of preserving fertility for men undergoing vasectomy or treatments that may compromise their fertility, such as chemotherapy, radiation therapy or surgery.
Cryopreservation of immature testicular tissue is a developing method to avail reproduction to young boys who need to have gonadotoxic therapy. Animal data are promising, since healthy offspring have been obtained after transplantation of frozen testicular cell suspensions or tissue pieces. However, none of the fertility restoration options from frozen tissue, i.e. cell suspension transplantation, tissue grafting and in vitro maturation (IVM) has proved efficient and safe in humans as yet.
Cryopreservation of whole moss plants, especially Physcomitrella patens, has been developed by Ralf Reski and coworkers and is performed at the International Moss Stock Center. This biobank collects, preserves, and distributes moss mutants and moss ecotypes.
Mesenchymal stromal cells (MSCs)Edit
MSCs, when transfused immediately within a few hours post-thawing, may show reduced function or show decreased efficacy in treating diseases as compared to those MSCs which are in log phase of cell growth (fresh). As a result, cryopreserved MSCs should be brought back into log phase of cell growth in in vitro culture before these are administered for clinical trials or experimental therapies. Re-culturing of MSCs will help in recovering from the shock the cells get during freezing and thawing. Various clinical trials on MSCs have failed which used cryopreserved products immediately post-thaw as compared to those clinical trials which used fresh MSCs.
Preservation of microbiology culturesEdit
Bacteria and fungi can be kept short-term (months to about a year, depending) refrigerated, however, cell division and metabolism is not completely arrested and thus is not an optimal option for long-term storage (years) or to preserve cultures genetically or phenotypically, as cell divisions can lead to mutations or sub-culturing can cause phenotypic changes. A preferred option, species-dependent, is cryopreservation. Nematode worms are the only multicellular eukaryotes that have been shown to survive cryopreservation.Shatilovich AV, Tchesunov AV, Neretina TV, Grabarnik IP, Gubin SV, Vishnivetskaya TA, Onstott TC, Rivkina EM (May 2018). "Viable Nematodes from Late Pleistocene Permafrost of the Kolyma River Lowland". Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections. 480 (1): 100–102. doi:10.1134/S0012496618030079. PMID 30009350.
Fungi, notably zygomycetes, ascomycetes and higher basidiomycetes, regardless of sporulation, are able to be stored in liquid nitrogen or deep-frozen. Crypreservation is a hallmark method for fungi that do not sporulate (otherwise other preservation methods for spores can be used at lower costs and ease), sporulate but have delicate spores (large or freeze-dry sensitive), are pathogenic (dangerous to keep metabolically active fungus) or are to be used for genetic stocks (ideally to have identical composition as the original deposit). As with many other organisms, cryoprotectants like DMSO or glycerol (e.g. filamentous fungi 10% glycerol or yeast 20% glycerol) are used. Differences between choosing cryoprotectants are species (or class) dependent, but generally for fungi penetrating cryoprotectants like DMSO, glycerol or polyethylene glycol are most effective (other non-penetrating ones include sugars mannitol, sorbitol, dextran, etc.). Freeze-thaw repetition is not recommended as it can decrease viability. Back-up deep-freezers or liquid nitrogen storage sites are recommended. Multiple protocols for freezing are summarized below (each uses screw-cap polypropylene cryotubes):
Many common culturable laboratory strains are deep-frozen to preserve genetically and phenotypically stable, long-term stocks. Sub-culturing and prolonged refrigerated samples may lead to loss of plasmid(s) or mutations. Common final glycerol percentages are 15, 20 and 25. From a fresh culture plate, one single colony of interest is chosen and liquid culture is made. From the liquid culture, the medium is directly mixed with equal amount of glycerol; the colony should be checked for any defects like mutations. All antibiotics should be washed from the culture before long-term storage. Methods vary, but mixing can be done gently by inversion or rapidly by vortex and cooling can vary by either placing the cryotube directly at −50 to −95 °C, shock-freezing in liquid nitrogen or gradually cooling and then storing at −80 °C or cooler (liquid nitrogen or liquid nitrogen vapor). Recovery of bacteria can also vary, namely if beads are stored within the tube then the few beads can be used to plate or the frozen stock can be scraped with a loop and then plated, however, since only little stock is needed the entire tube should never be completely thawed and repeated freeze-thaw should be avoided. 100% recovery is not feasible regardless of methodology.
The microscopic soil-dwelling nematode roundworms Panagrolaimus detritophagus and Plectus parvus are the only eukaryotic organisms that have been proven to be viable after long-term cryopreservation to date. In this case, the preservation was natural rather than artificial, due to permafrost.
- Pegg DE (January 1, 2007). "Principles of cryopreservation". Methods in Molecular Biology. 368: 39–57. doi:10.1007/978-1-59745-362-2_3. ISBN 978-1-58829-377-0. PMID 18080461.
- Sambu S (June 25, 2015). "A Bayesian approach to optimizing cryopreservation protocols". PeerJ. 3: e1039. doi:10.7717/peerj.1039. PMC 4485240. PMID 26131379.
- Costanzo JP, Lee RE, Wright MF (December 1991). "Glucose loading prevents freezing injury in rapidly cooled wood frogs" (PDF). The American Journal of Physiology. 261 (6 Pt 2): R1549–53. doi:10.1152/ajpregu.1991.261.6.R1549. PMID 1750578.
- Lovelock JE (March 1953). "The haemolysis of human red blood-cells by freezing and thawing". Biochimica et Biophysica Acta. 10 (3): 414–26. doi:10.1016/0006-3002(53)90273-X. PMID 13058999.
- Fuller BJ, Lane N, Benson EE, eds. (2004). Life in the Frozen State. CRC Press. p. 7. ISBN 978-0203647073.
- Mazur P (May 1970). "Cryobiology: the freezing of biological systems". Science. 168 (3934): 939–49. Bibcode:1970Sci...168..939M. doi:10.1126/science.168.3934.939. PMID 5462399.
- "The Cryobiological Case for Cryonics" (PDF). Cryonics. Vol. 9(3) no. 92. Alcor Life Extension Foundation. March 1988. p. 27.
- Andjus RK, Smith AU (June 1955). "Reanimation of adult rats from body temperatures between 0 and + 2 degrees C". The Journal of Physiology. 128 (3): 446–72. doi:10.1113/jphysiol.1955.sp005318. PMC 1365897. PMID 13243342.
- "Fatherhood After Death Has Now Been Proved Possible". Cedar Rapids Gazette. April 9, 1954.
- Polge C (December 1957). "Low-temperature storage of mammalian spermatozoa". Proceedings of the Royal Society of London. Series B, Biological Sciences. 147 (929): 498–508. Bibcode:1957RSPSB.147..498P. doi:10.1098/rspb.1957.0068. PMID 13494462.
- Mazur P (July 1963). "Studies on rapidly frozen suspensions of yeast cells by differential thermal analysis and conductometry" (PDF). Biophysical Journal. 3 (4): 323–53. Bibcode:1963BpJ.....3..323M. doi:10.1016/S0006-3495(63)86824-1. PMC 1366450. PMID 13934216.
- "Dear Dr. Bedford (and those who will care for you after I do)". Cryonics. July 1991. Retrieved 2009-08-23.
- Perry RM (October 2014). "Suspension Failures – Lessons from the Early Days". ALCOR: Life Extension Foundation. Retrieved August 29, 2018.
- Mazur P (September 1984). "Freezing of living cells: mechanisms and implications". The American Journal of Physiology. 247 (3 Pt 1): C125-42. Bibcode:1957RSPSB.147..498P. doi:10.1098/rspb.1957.0068. PMID 6383068.
- Vutyavanich T, Piromlertamorn W, Nunta S (April 2010). "Rapid freezing versus slow programmable freezing of human spermatozoa". Fertility and Sterility. 93 (6): 1921–8. doi:10.1016/j.fertnstert.2008.04.076. PMID 19243759.
- "dead link". Archived from the original on 2009-05-26.
- Deller RC, Vatish M, Mitchell DA, Gibson MI (February 3, 2014). "Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing". Nature Communications. 5: 3244. Bibcode:2014NatCo...5.3244D. doi:10.1038/ncomms4244. PMID 24488146.
- Thompson M, Nemits M, Ehrhardt R (May 2011). "Rate-controlled Cryopreservation and Thawing of Mammalian Cells". Protocol Exchange. doi:10.1038/protex.2011.224.
- Rall WF, Fahy GM (February 14–20, 1985). "Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification". Nature. 313 (6003): 573–5. Bibcode:1985Natur.313..573R. doi:10.1038/313573a0. PMID 3969158.
- "Alcor: The Origin of Our Name" (PDF). Alcor Life Extension Foundation. Winter 2000. Retrieved August 25, 2009.
- Kuleshova LL, Wang XW, Wu YN, Zhou Y, Yu H (2004). "Vitrification of encapsulated hepatocytes with reduced cooling and warming rates". Cryo Letters. 25 (4): 241–54. PMID 15375435.
- Kuleshova L, Gianaroli L, Magli C, Ferraretti A, Trounson A (December 1999). "Birth following vitrification of a small number of human oocytes: case report". Human Reproduction. 14 (12): 3077–9. doi:10.1093/humrep/14.12.3077. PMID 10601099.
- Bhat SN, Sharma A, Bhat SV (December 2005). "Vitrification and glass transition of water: insights from spin probe ESR". Physical Review Letters. 95 (23): 235702. arXiv:cond-mat/0409440. Bibcode:2005PhRvL..95w5702B. doi:10.1103/PhysRevLett.95.235702. PMID 16384318.
- Fahy GM, Wowk B, Pagotan R, Chang A, Phan J, Thomson B, Phan L (July 2009). "Physical and biological aspects of renal vitrification". Organogenesis. 5 (3): 167–75. doi:10.4161/org.5.3.9974. PMC 2781097. PMID 20046680.
- Geddes L (Sep 11, 2013). "Heart of glass could be key to banking organs". New Scientist.
- Flynn M (Oct 10, 2018). "Heart of Ice". BOSS Magazine.
- US9314015B2, Stephen Van Sickle, Tanya Jones, "Method and apparatus for prevention of thermo-mechanical fracturing in vitrified tissue using rapid cooling and warming by persufflation", published Apr 19, 2013
- Suszynski TM, Rizzari MD, Scott WE, Tempelman LA, Taylor MJ, Papas KK (June 2012). "Persufflation (or gaseous oxygen perfusion) as a method of organ preservation". Cryobiology. 64 (3): 125–43. doi:10.1016/j.cryobiol.2012.01.007. PMC 3519283. PMID 22301419.
- Lee JY, Lee JE, Kim DK, Yoon TK, Chung HM, Lee DR (February 2010). "High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem cells". Fertility and Sterility. 93 (3): 976–85. doi:10.1016/j.fertnstert.2008.10.017. PMID 19022437.
- Devlin H. "Cryonics: Does It Offer Humanity a Chance to Return from the Dead?". thegaurdian.com. The Guardian. Retrieved 17 April 2017.
- Planer NEWS and Press Releases > 'Twins' born 16 years apart.[permanent dead link] January 6, 2006.
- "Genetics & IVF Institute". Givf.com. Archived from the original on December 6, 2012. Retrieved July 27, 2009.
- Riggs R, Mayer J, Dowling-Lacey D, Chi TF, Jones E, Oehninger S (January 2010). "Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human embryos". Fertility and Sterility. 93 (1): 109–15. doi:10.1016/j.fertnstert.2008.09.084. PMID 19027110.
- Isachenko V, Lapidus I, Isachenko E, Krivokharchenko A, Kreienberg R, Woriedh M, et al. (August 2009). "Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation". Reproduction. 138 (2): 319–27. doi:10.1530/REP-09-0039. PMID 19439559.
- Oktay K, Oktem O (February 2010). "Ovarian cryopreservation and transplantation for fertility preservation for medical indications: report of an ongoing experience". Fertility and Sterility. 93 (3): 762–8. doi:10.1016/j.fertnstert.2008.10.006. PMID 19013568.
- Livebirth after orthotopic transplantation of cryopreserved ovarian tissue[permanent dead link] The Lancet, September 24, 2004
- Lan C, Xiao W, Xiao-Hui D, Chun-Yan H, Hong-Ling Y (February 2010). "Tissue culture before transplantation of frozen-thawed human fetal ovarian tissue into immunodeficient mice". Fertility and Sterility. 93 (3): 913–9. doi:10.1016/j.fertnstert.2008.10.020. PMID 19108826.
- Glujovsky D, Riestra B, Sueldo C, Fiszbajn G, Repping S, Nodar F, Papier S, Ciapponi A. Vitrification versus slow freezing for women undergoing oocyte cryopreservation" Cochrane Database of Systematic Reviews 2014, Issue 8. Art. No.: CD010047. doi:10.1002/14651858.CD010047.pub2
- Planer NEWS and Press Releases > Child born after 22 year semen storage using Planer controlled rate freezer Archived 2012-09-08 at Archive.today 14/10/2004
- Wyns C, Curaba M, Vanabelle B, Van Langendonckt A, Donnez J (2010). "Options for fertility preservation in prepubertal boys". Human Reproduction Update. 16 (3): 312–28. doi:10.1093/humupd/dmp054. PMID 20047952.
- Schulte J, Reski R (2004). "High throughput cryopreservation of 140,000 Physcomitrella patens mutants". Plant Biology. Plant Biotechnology, Freiburg University, Freiburg, Germany. 6 (2): 119–27. doi:10.1055/s-2004-817796. PMID 15045662.
- "Mosses, deep frozen". ScienceDaily.
- François M, Copland IB, Yuan S, Romieu-Mourez R, Waller EK, Galipeau J (February 2012). "Cryopreserved mesenchymal stromal cells display impaired immunosuppressive properties as a result of heat-shock response and impaired interferon-γ licensing". Cytotherapy. 14 (2): 147–52. doi:10.3109/14653249.2011.623691. PMC 3279133. PMID 22029655.
- Weisberger M (2018). "Worms Frozen for 42,000 Years in Siberian Permafrost Wriggle to Life". Live Science.
- "Archived copy" (PDF). Archived from the original (PDF) on 2014-05-17. Retrieved 2014-05-15.CS1 maint: archived copy as title (link)
- Freeze-Drying and Cryopreservation of Bacteria
- "Addgene: Protocol - How to Create a Bacterial Glycerol Stock". Addgene.org. Retrieved 9 September 2015.
- "Archived copy". Archived from the original on 2013-09-07. Retrieved 2014-05-15.CS1 maint: archived copy as title (link)
- Engelmann F, Dulloo ME, Astorga C, Dussert S, Anthony F, eds. (2007). Conserving coffee genetic resources. Bioversity International, CATIE, IRD. p. 61.
- Panis B, Tien Thinh N (2001). Cryopreservation of Musa germplasm. INIBAP (now Bioversity International). p. 45.
- ReproTech Limited (2012). "Fertility Preservation". ReproTech Limited. Archived from the original on 2012-09-04.
- Nakasone KK, Peterson SW, Jong SC (2004). "Preservation and distribution of fungal cultures.". Biodiversity of fungi: inventory and monitoring methods. Amsterdam: Elsevier Academic Press. pp. 37–47.
- Perry SF (1995). "Freeze-drying and cryopreservation of bacteria". Methods in Molecular Biology. Clifton, N.J. 38: 21–30. doi:10.1385/0-89603-296-5:21. ISBN 0-89603-296-5. PMID 7647859.