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3D structure of Ca2+-bound Calmodulin (1OSA.pdb)
The helix loop helix structure of the calcium binding EF hand motif

Calmodulin (CaM) (an abbreviation for calcium-modulated protein) is a multifunctional intermediate calcium-binding messenger protein expressed in all eukaryotic cells.[1] It is an intracellular target of the secondary messenger Ca2+, and the binding of Ca2+ is required for the activation of calmodulin. Once bound to Ca2+, calmodulin acts as part of a calcium signal transduction pathway by modifying its interactions with various target proteins such as kinases or phosphatases.[2][3][4]



Calmodulin is a small, highly conserved protein that is 148 amino acids long (16.7 KDa). The protein has two approximately symmetrical globular domains each containing a pair of EF-hand motifs (the N- and C-domain) separated by a flexible linker region for a total of four Ca2+ binding sites.[5] Each EF-hand motif allows calmodulin to sense intracellular calcium levels by binding one Ca2+ ion. Calcium ion binding regions are found in the following positions in the sequence of amino acids: 21-32, 57-68, 94-105 and 130-141; each region that calcium binds to is exactly 12 amino acids long. These regions are located between two alpha helices in the EF-hand motifs, the first two regions (21-32 and 57-68) are on one side of the linker region the other two (94-105 and 130-141) are on the other side.[4]

Importance of flexibility in calmodulinEdit

Calmodulin binds such a wide variety of target proteins, making it especially important for it to have flexibility. Though Calmodulin's flexibility is more evident when it is bound to a target protein, NMR studies have shown that the linker region of Calmodulin is flexible, even when it is not bound to a target protein. Another important characteristic of calmodulin that allows it to bind a large variety of target proteins is the generic shape of the non-polar grooves in the binding sites. Since the non-polar grooves are generic, they don't require the target proteins to have any specific sequence of amino acids allowing a larger variety of target proteins to be bound. Together, these two structural characteristics of calmodulin allow it to flexibly bind target proteins with various shapes and amino acid sequences.[5] For example, calmodulin binds both NMDA receptors and potassium channels which differ in length by about 50 amino acid residues.[6][7]

On the left: Calmodulin with four calcium ions bound (1CLL.pdb). On the right: Troponin C with four calcium ions bound (1TCF.pdb).

Similarity to troponin CEdit

Calmodulin's structure is very similar to the structure of troponin C (which is another calcium binding protein). They are both members of the EFh superfamily. Troponin C, like Calmodulin, has two globular domains that are connected by a linker region.[8] However, Troponin C and Calmodulin differ in the length of the linker region; the linker region of Calmodulin is smaller than that of Troponin C.[8] These remarkably similar structures are an example of how the EF hand motif is highly conserved in calcium binding proteins. Though they have similar structures, their functions are very different. Troponin C has a very specific function (to elicit a conformational change in Troponin I) ultimately causing a contraction in skeletal muscles. Calmodulin, evolved to bind a wider variety of target proteins, allowing it to play a role in many physiological events.[5][8]


This images shows conformational changes in calmodulin. On the left is calmodulin without calcium and on the right is calmodulin with calcium. Sites that bind target proteins are indicated by red stars.


Solution structure of Calcium calmodulin C-terminal domain
Solution structure of Calcium-calmodulin N-terminal domain

Up to four calcium ions are bound by calmodulin via its four EF hand motifs.[5] EF hands supply an electronegative environment for ion coordination. After calcium binding, hydrophobic methyl groups from methionine residues become exposed on the protein via conformational change. Using both X-Ray and NMR studies, scientists were able to determine that the conformational changes occurred in the alpha-helices of the EF motif, which changes the binding affinity for target proteins. When the alpha helices are perpendicular to one another, the Calmodulin is in an open conformation giving it a higher affinity for target proteins.[9] More specifically, this conformational change presents hydrophobic surfaces, which can in turn bind to Basic Amphiphilic Helices (BAA helices) on the target protein. These helices contain complementary hydrophobic regions. The flexibility of calmodulin's hinged region allows the molecule to wrap around its target.[5] This property allows it to tightly bind to a wide range of different target proteins. The C-domain of calmodulin has a higher affinity for calcium than does the N-domain.

Dynamic featuresEdit

The C-terminal domain solution structure is similar to the X-ray crystal structure, while the EF hands of the N-terminal domain are considerably less open to the X-ray crystal structure. This indicates that Ca2+ binding causes a larger conformational change in the N-domain than in the C-domain. The backbone flexibility within calmodulin is key to its ability to bind a wide range of targets.[10] Protein domains, connected by intrinsically disordered flexible linker domains, induce long-range allostery, or the conformational change of a protein by ligand binding to an allosteric site (a site other than the functional site), via protein domain dynamics.[10]

Role in animalsEdit

Calmodulin mediates many crucial processes such as inflammation, metabolism, apoptosis, smooth muscle contraction, intracellular movement, short-term and long-term memory, and the immune response.[11][12] Calcium participates in an intracellular signaling system by acting as a diffusible second messenger to the initial stimuli. It does this by binding various targets in the cell including a large number of enzymes, ion channels, aquaporins and other proteins.[4] Calmodulin is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes, but it is always found intracellularly.[12] Many of the proteins that Calmodulin binds are unable to bind calcium themselves, and use calmodulin as a calcium sensor and signal transducer. Calmodulin can also make use of the calcium stores in the endoplasmic reticulum, and the sarcoplasmic reticulum. Calmodulin can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which has potential to modulate its actions.

Specific examplesEdit

Role in smooth muscle contractionEdit

Calmodulin bound to a peptide from MLC Kinase (2LV6.pdb)

Calmodulin plays an important role in excitation contraction (EC) coupling and the initiation of the cross-bridge cycling in smooth muscle, ultimately causing smooth muscle contraction.[13] In order to activate contraction of smooth muscle, the head of the myosin light chain must be phosphorylated. This phosphorylation is done by myosin light chain (MLC) kinase. This MLC Kinase is activated by a calmodulin when it is bound by calcium, thus making smooth muscle contraction dependent on the presence of calcium, through the binding of calmodulin and activation of MLC kinase.[13]

Another way that calmodulin affects muscle contraction is by controlling the movement of Ca2+ across both the cell and sarcoplasmic reticulum membranes. The Ca2+ channels, such as the ryanodine receptor of the sarcoplasmic reticulum, can be inhibited by calmodulin bound to calcium, thus affecting the overall levels of calcium in the cell.[14] Calcium pumps take calcium out of the cytoplasm and/or store it in the endoplasmic reticulum and this control helps regulate many downstream processes.

This is a very important function of calmodulin because it indirectly plays a role in every physiological process that is affected by smooth muscle contraction such as digestion and contraction of arteries (which helps distribute blood and regulate blood pressure).[15]

Role in metabolismEdit

Calmodulin plays an important role in the activation of phosphorylase kinase, which ultimately leads to glucose being cleaved from glycogen by glycogen phosphorylase.[16]

Calmodulin also plays an important role in lipid metabolism by affecting Calcitonin. Calcitonin is a polypeptide hormone that lowers blood Ca2+ levels and activates G Protein cascades that leads to the generation of cAMP. The actions of calcitonin can be blocked by inhibiting the actions of calmodulin, suggesting that calmodulin plays a crucial role in the activation of calcitonin.[16]

Role in short-term and long-term memoryEdit

Ca2+/calmodulin-dependent protein kinase II (CaMKII) plays a crucial role in a type of synaptic plasticity called long-term potentiation (LTP) and requires the presence of calcium/calmodulin. CaMKII contributes to the phosphorylation of an AMPA receptor which increases the sensitivity of AMPA receptors.[17] Furthermore, research shows that inhibiting CaMKII interferes with LTP.[17]

Role in plantsEdit

Sorghum plant contains temperature-responsive genes. These genes help the plant adapt in extreme weather conditions such as hot and dry environments.

While yeasts have only a single CaM gene, plants and vertebrates contain an evolutionarily conserved form of CaM genes. The difference between plants and animals in Ca2+ signaling is that the plants contain an extended family of the CaM in addition to the evolutionarily conserved form.[18] Calmodulins play an essential role in plant development and adaptation to environmental stimuli.

Calcium plays a key role in the structural integrity of the cell wall and the membrane system of the cell. However, high calcium levels can be toxic to a plant's cellular energy metabolism and, hence, the Ca2+ concentration in the cytosol is maintained at a submicromolar level by removing the cytosolic Ca2+ to either the apoplast or the lumen of the intracellular organelles. Ca2+ pulses created due to increased influx and efflux act as cellular signals in response to external stimuli such as hormones, light, gravity, abiotic stress factors and also interactions with pathogens.

CMLs (CaM-related proteins)Edit

Plants contain CaM-related proteins (CMLs) apart from the typical CaM proteins. The CMLs have about 15% amino acid similarity with the typical CaMs. Arabidopsis thaliana contains about 50 different CML genes which leads to the question of what purpose these diverse ranges of proteins serve in the cellular function. All plant species exhibit this diversity in the CML genes. The different CaMs and CMLs differ in their affinity to bind and activate the CaM-regulated enzymes in vivo. The CaM or CMLs are also found to be located in different organelle compartments.

Plant growth and developmentEdit

1) DWF1 in Arabidopsis is a protein that plays an enzymatic role in the biosynthesis of a steroid hormone in plants that is required for plant growth called, brassinosteroid. An interaction occurs between CaM and DWF1 and DWF1 unable to bind CaM is unable to produce a regular growth phenotype in plants. Hence, CaM is essential for the DWF1 function in plant growth.

2) CaM binding proteins are also known to regulate reproductive development in plants. For instance, the CaM-binding protein kinase in tobacco acts as a negative regulator of flowering. However, these CaM-binding protein kinase are also present in the shoot apical meristem of tobacco and a high concentration of these kinases in the meristem causes a delayed transition to flowering in the plant.

3) S-locus receptor kinase (SRK) is another protein kinase that interacts with CaM. SRK is involved in the self-incompatibility responses involved in pollen-pistil interactions in Brassica.

4) CaM targets in Arabidopsis are also involved in pollen development and fertilization. Ca2+ transporters are essential for pollen tube growth. Hence, a constant Ca2+ gradient is maintained at the apex of pollen tube for elongation during the process of fertilization. Similarly, CaM is also essential at the pollen tube apex it's primarily role here involves guidance of the pollen tube growth.

Interaction with microbesEdit

1) Nodule formation: Ca2+ plays a significantly important role in nodule formation in legumes. Nitrogen is an essential element required in plants and many legumes, unable to fix nitrogen independently, pair symbiotically with nitrogen-fixing bacteria that reduce nitrogen to ammonia. This legume-rhizobia interaction establishment requires the Nod factor that is produced by the rhizobium bacteria. The Nod factor is recognized by the root hair cells that are involved in the nodule formation in legumes. Ca2+ responses of varied nature are characterized to be involved in the Nod factor recognition. There is a Ca2+ flux at the tip of the root hair initially followed by repetitive oscillation of Ca2+ in the cytosol and also Ca2+ spike occurs around the nucleus. DMI3, an essential gene for Nod factor signaling functions downstream of the Ca2+ spiking signature, might be recognizing the Ca2+ signature. Further, several CaM and CML genes in Medicago and Lotus are expressed in nodules.

2) Pathogen Defense: Among the diverse range of defense strategies plants utilize against pathogens, Ca2+ signaling is very increasingly common. Free Ca2+ levels in the cytoplasm increases in response to a pathogenic infection. Ca2+ signatures of this nature usually activate the plant defense system by inducing defense-related genes and the hypersensitive cell death. CaMs, CMLs and CaM-binding proteins are some of the recently identified elements of the plant defense signaling pathways. Several CML genes in tobacco, bean and tomato are responsive to pathogens. CML43 is a CaM-related protein that, as isolated from APR134 gene in the disease-resistant leaves of Arabidopsis for gene expression analysis, is rapidly induced when the leaves are inoculated with Pseudomonas syringae. These genes are also found in tomatoes (Solanum lycopersicum). The CML43 from the APR134 also binds to Ca2+ ions in vitro which shows that CML43 and APR134 are, hence, involved in the Ca2+-dependent signaling during the plant immune response to bacterial pathogens.[19] The CML9 expression in Arabidopsis thaliana is rapidly induced by phytopathogenic bacteria, flagellin and salicylic acid.[20] Expression of soybean SCaM4 and SCaM5 in transgenic tobacco and Arabidopsis causes an activation of genes related to pathogen resistance and also results in enhanced resistance to a wide spectrum of pathogen infection. The same is not true for soybean SCaM1 and SCaM2 that are highly conserved CaM isoforms. The AtBAG6 protein is a CaM-binding protein that binds to CaM only in the absence of Ca2+ and not in the presence of it. AtBAG6 is responsible for the hypersensitive response of programmed cell death in order to prevent the spread of pathogen infection or to restrict pathogen growth. Mutations in the CaM binding proteins can lead to severe effects on the defense response of the plants towards pathogen infections. Cyclic nucleotide-gated channels (CNGCs) are functional protein channels in the plasma membrane that have overlapping CaM binding sites transport divalent cations such as Ca2+. However, the exact role of the positioning of the CNGCs in this pathway for plant defense is still unclear.

Abiotic stress response in plantsEdit

Change in intracellular Ca2+ levels is used as a signature for diverse responses towards mechanical stimuli, osmotic and salt treatments, and cold and heat shocks. Different root cell types show a different Ca2+ response to osmotic and salt stresses and this implies the cellular specificities of Ca2+ patterns. In response to external stress CaM activates glutamate decarboxylase (GAD) that catalyzes the conversion of L-glutamate to GABA. A tight control on the GABA synthesis is important for plant development and, hence, increased GABA levels can essentially affect plant development. Therefore, external stress can affect plant growth and development and CaM are involved in that pathway controlling this effect.[citation needed]

Plant examplesEdit


The plant sorghum is well established model organism and can adapt in hot and dry environments. For this reason, it is used as a model to study calmodulin's role in plants.[21] Sorghum, contains seedlings that express a glycine-rich RNA-binding protein, SbGRBP. This particular protein can be modulated by using heat as a stressor. Its unique location in the cell nucleus and cytosol demonstrates interaction with calmodulin that requires the use of Ca2+.[22] By exposing the plant to versatile stress conditions, it can cause different proteins that enable the plant cells to tolerate environmental changes to become repressed. These modulated stress proteins are shown to interact with CaM. The CaMBP genes expressed in the sorghum are depicted as a “model crop” for researching the tolerance to heat and drought stress.


In an Arabidopsis thaliana study, hundreds of different proteins demonstrated the possibility to bind to CaM in plants.[21]

Family membersEdit

Other calcium-binding proteinsEdit

Calmodulin belongs to one of the two main groups of calcium-binding proteins, called EF hand proteins. The other group, called annexins, bind calcium and phospholipid (e.g., lipocortin). Many other proteins bind calcium, although binding calcium may not be considered their principal function in the cell.

See alsoEdit


  1. ^ Stevens FC (1983). "Calmodulin: an introduction". Can. J. Biochem. Cell Biol. 61 (8): 906–10. doi:10.1139/o83-115. PMID 6313166.
  2. ^ Chin D, Means AR (2000). "Calmodulin: a prototypical calcium sensor". Trends Cell Biol. 10 (8): 322–8. doi:10.1016/S0962-8924(00)01800-6. PMID 10884684.
  3. ^ Purves, Dale; Augustine, George; Fitzpatrick, David; Hall, William; LaMantia, Anthony-Samuel; White, Leonard (2012). Neuroscience. Massachusetts: Sinauer Associates, Inc. pp. 95, 147, 148. ISBN 9780878936953.
  4. ^ a b c "CALM1 - Calmodulin - Homo sapiens (Human) - CALM1 gene & protein". Retrieved 2016-02-23.
  5. ^ a b c d e "Calmodulin". Retrieved 2016-02-23.
  6. ^ M.A., RCSB Protein Data Bank, Ataman, Z.A., Gakhar, L., Sorensen, B.R., Hell, J.W., Shea,. "RCSB PDB - 2HQW: Crystal Structure of Ca2+/Calmodulin bound to NMDA Receptor NR1C1 peptide Structure Summary Page". Retrieved 2016-03-14.
  7. ^ J.F., RCSB Protein Data Bank, Zhang, M., Meng, X.Y., Cui, M., Pascal, J.M., Logothetis, D.E., Zhang,. "RCSB PDB - 4QNH: Calcium-calmodulin (T79D) complexed with the calmodulin binding domain from a small conductance potassium channel SK2-a Structure Summary Page". Retrieved 2016-03-14.
  8. ^ a b c "Calmodulin". Retrieved 2016-02-23.
  9. ^ "Calmodulin Target Database". Retrieved 2016-02-01.
  10. ^ a b Chou JJ, Li S, Klee CB, Bax A (November 2001). "Solution structure of Ca(2+)-calmodulin reveals flexible hand-like properties of its domains". Nat. Struct. Biol. 8 (11): 990–7. doi:10.1038/nsb1101-990. PMID 11685248.
  11. ^ "Home Page for Calmodulin". Retrieved 2016-02-23.
  12. ^ a b McDowall, Jennifer. "Calmodulin". InterPro Protein Archive. Retrieved 23 February 2016.
  13. ^ a b Tansey, M. G.; Luby-Phelps, K.; Kamm, K. E.; Stull, J. T. (1994-04-01). "Ca(2+)-dependent phosphorylation of myosin light chain kinase decreases the Ca2+ sensitivity of light chain phosphorylation within smooth muscle cells". Journal of Biological Chemistry. 269 (13): 9912–9920. ISSN 0021-9258. PMID 8144585.
  14. ^ Walsh, M. P. (1994-06-15). "Calmodulin and the regulation of smooth muscle contraction". Molecular and Cellular Biochemistry. 135 (1): 21–41. doi:10.1007/bf00925958. ISSN 0300-8177. PMID 7816054.
  15. ^ Martinsen, A; Dessy, C; Morel, N (2014-10-31). "Regulation of calcium channels in smooth muscle: New insights into the role of myosin light chain kinase". Channels. 8 (5): 402–413. doi:10.4161/19336950.2014.950537. ISSN 1933-6950. PMC 4594426. PMID 25483583.
  16. ^ a b Nishizawa, Y; Okui, Y; Inaba, M; Okuno, S; Yukioka, K; Miki, T; Watanabe, Y; Morii, H (1988-10-01). "Calcium/calmodulin-mediated action of calcitonin on lipid metabolism in rats". Journal of Clinical Investigation. 82 (4): 1165–1172. doi:10.1172/jci113713. ISSN 0021-9738. PMC 442666. PMID 2844851.
  17. ^ a b Lledo, P M; Hjelmstad, G O; Mukherji, S; Soderling, T R; Malenka, R C; Nicoll, R A (1995-11-21). "Calcium/calmodulin-dependent kinase II and long-term potentiation enhance synaptic transmission by the same mechanism". Proceedings of the National Academy of Sciences of the United States of America. 92 (24): 11175–11179. doi:10.1073/pnas.92.24.11175. ISSN 0027-8424. PMC 40594. PMID 7479960.
  18. ^ Ranty, Benoît, Didier Aldon, and Jean-Philippe Galaud. “Plant Calmodulins and Calmodulin-Related Proteins: Multifaceted Relays to Decode Calcium Signals.” Plant Signaling & Behavior 1.3 (2006): 96–104. Print.
  19. ^ Chiasson, David; Ekengren, Sophia K.; Martin, Gregory B.; Dobney, Stephanie L.; Snedden, Wayne A. (2005-08-01). "Calmodulin-like Proteins from Arabidopsis and Tomato are Involved in Host Defense Against Pseudomonas syringae pv. tomato". Plant Molecular Biology. 58 (6): 887–897. doi:10.1007/s11103-005-8395-x. ISSN 0167-4412. PMID 16240180.
  20. ^ Leba, Louis-Jérôme; Cheval, Cécilia; Ortiz-Martín, Inmaculada; Ranty, Benoit; Beuzón, Carmen R.; Galaud, Jean-Philippe; Aldon, Didier (2012-09-01). "CML9, an Arabidopsis calmodulin-like protein, contributes to plant innate immunity through a flagellin-dependent signalling pathway". The Plant Journal. 71 (6): 976–989. doi:10.1111/j.1365-313x.2012.05045.x. ISSN 1365-313X. PMID 22563930.
  21. ^ a b "Mapping QTLs associated with drought resistance in sorghum (Sorghum bicolor L. Moench) (PDF Download Available)". ResearchGate. Retrieved 2017-11-05.
  22. ^ Singh, Supreet; Virdi, Amardeep Singh; Jaswal, Rajdeep; Chawla, Mrinalini; Kapoor, Sanjay; Mohapatra, Samar B.; Manoj, Narayanan; Pareek, Ashwani; Kumar, Sanjay (2017-06-01). "A temperature-responsive gene in sorghum encodes a glycine-rich protein that interacts with calmodulin". Biochimie. 137 (Supplement C): 115–123. doi:10.1016/j.biochi.2017.03.010.

External linksEdit