Pyridine is a heterocyclic organic compound. It has the chemical formula C5H5N, consisting of a benzene core where one -CH group is replaced with a Nitrogen atom. Pyridine appears as colorless, malodorous liquid.[1]
The Hantzsch pyridine synthesis or Hantzsch dihydropyridine synthesis is a multi-component organic reaction between an aldehyde such as formaldehyde, 2 equivalents of a β-keto ester such as ethyl acetoacetate and a nitrogen donor such as ammonium acetate or ammonia. The original reaction was reported by Arthur Randolph Hantzsch in 1881 and involved three components: acetoacetic ester, benzaldehyde, and ammonia or ammonium salts in refluxing ethanol.[2] The initial reaction product is a dihydropyridine. Hantzsch's original reaction is the most widely used protocol to form dialkyl 1,4-dihydro-2,6 dimethylpyridine-3,5-dicarboxylates with a variety of substituents in position 4 (the carbon most removed from the nitrogen).[3] This initial reaction product can be oxidized in a subsequent step to a pyridine. The driving force for this second reaction step is aromatization.
A 1,4-dihydropyridine dicarboxylate is also called a 1,4-DHP compound or Hantzsch ester. 1,4-DHPs make up an important class of bioactive molecules.[4] These molecules resemble reduced nicotinamide adenine dinucleotide (NADH) and this structural similarity has led to progress in the chemistry of Hantzsch pyridines.Cite error: A <ref> tag is missing the closing </ref> (see the help page). 1,4-DHPs are also an important class of calcium channel blockers [4] and as such a structural feature in nifedipine, amlodipine or nimodipine. These compounds possess the ability to shorten the transmembrane calcium current, aiding in the treatment of angina and hypertension.[3]
The Hantzsch pyridine reaction has been demonstrated to proceed in water as reaction solvent and with direct aromatization by ferric chloride, manganese dioxide, or potassium permanganate in a one-pot synthesis.[3]
Mechanism edit
At least five significant pathways have been proposed for the Hantzch reaction synthesis of 1,4-dihydropyridine. Low yield and unexpected products may arise under varying reactants and reaction conditions. Previous studies have tested the reactions of preformed intermediates to determine the most likely mechanism and design successful syntheses.[5] An early study into the mechanism using 13C and 15N NMR indicated the intermediacy of the chalcone 6 and enamine 3. This data suggested the following route for the reaction.[6]
Later research using mass spectrometry monitoring with charge-tagged reactants supported intermediate pathway A as a likely route and showed evidence that the reaction followed two additional intermediate pathways which converge to precursor 7. [5] Reagents likely influence the route taken as when the methyl group of 1 is replaced by an electron-withdrawing group, the reaction instead proceeds through a diketone intermediate.[2]
Optimization of Reaction Conditions edit
The classical method for synthesis of Hantzsch 1,4-dihydropyridines, which involves a one-pot condensation of aldehydes with ethyl acetoacetate and ammonia, have several drawbacks such as harsh reaction conditions, long reaction times, and generally low yield of products. A synthesis of 1,4-dihydropyridines in aqueous micelles catalyzed by PTSA under ultrasonic irradiation. Using condensation of benzaldehyde, ethyl acetoacetate and ammonium acetate as a model, experiments have proven that when catalyzed by p-Toluenesulfonic acid (PTSA) under ultrasonic irradiation, the reaction can have a product yield of 96% in aqueous (SDS, 0.1M). The reaction had also been carried out in various solvent system, and it was discovered that the ultrasonic irradiation in aqueous micelles gave better yields than in solvents such as methanol, ethanol, THF. Using the optimized reaction conditions, a series of 1,4-dihydropyridine were synthesized, and they all have a reaction yield above 90%. [7]
Aromatization edit
Oxidation of 1,4-DHPs accounts for one of the easiest ways of accessing pyridine derivatives. [8]Common oxidants used to promote aromatization of 1,4-DHPs are CrO3, KMnO4, and HNO3.[9] However, aromatization is often accompanied by: low chemical yields, strong oxidative conditions, burdensome workups, the formation of side products, or the need of excess oxidant.[9][10] As such, particular attention has been paid to developing methods of aromatization to yield pyridine derivatives under milder and efficient conditions. Such conditions include, but are not limited to: iodine in refluxing methanol,[9] chromium dioxide(CrO2), [10] sodium chlorite,[11], and under metal-free, photochemical conditions using both UV-light and visible light.[12] Upon metabolism, 1,4-DHP based antihypertensive drugs undergo oxidation by way of cytochrome P-450 in the liver and are thus converted to their pyridine derivatives.[9] As a result, particular attention has been paid to the aromatization of 1,4-DHPs as a means to understand biological systems and so as to develop new methods of accessing pyridines.[11]
Green Chemistry edit
As a Multi-component reaction, the Hantzch pyridine synthesis is much more atom efficient with a simpler number of reaction steps than a linear-strategy synthesis.In recent years, research has looked to make this an even more environmentally friendly reaction by investigating “greener” solvents and reaction conditions.[13] One line of study has experimented with using ionic liquids as catalysts for room temperature reactions. Ionic liquids are an easy to handle and non-toxic option to replace traditional catalysts. Additionally, this catalyst lead to a high yield at room temperature, reducing the impact of heating the reaction for an extended time. A second study used ceric ammonium nitrate(CAN) as an alternate catalyst and achieved a solvent-free room temperature reaction.[14]
- ^ "Pyridine". PubChem. National Center for Biotechnology Information. Retrieved 25 May 2021.
- ^ a b Saini, Anil (February 2008). "Hantzsch reaction: Recent advances in Hantzsch 1,4-dihydropyridines" (PDF). Journal of Scientific and Industrial Research. 67: 95-111.
- ^ a b c Eynde, Jean Jacques Vanden (2008). "Synthesis and Aromatization of Hantzsch 1,4-Dihydropyridines under Microwave Irradiation. An Overview". Molecules. 8: 381–391. doi:10.3390/80400381.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ a b Vijesh, A. M. (2011). "Hantzsch reaction: Synthesis and characterization of some new 1,4-dihydropyridine derivatives as potent antimicrobial and antioxidant agents". European Journal of Medicinal Chemistry. 46: 5591-5597. doi:10.1016/j.ejmech.2011.09.026.
- ^ a b Santos, Vanessa G.; Godoi, Marla N.; Regiani, Thais; Gama, Fernando H.S; Coelho, Mirela B.; de Souza, Rodrigo O. M. A.; Eberlin, Marcos N.; Garden, Simon J. (1 September 2014). "The Multicomponent Hantzsch Reaction: Comprehensive Mass Spectrometry Monitoring Using Charge-Tagged Reagents". Chemistry - A European Journal (20): 12808–12816. doi:10.1002/chem.201303065.
- ^ Katrinsky, Alan R.; Ostercamp, Daryl L.; Yousaf, Taher I. (1986). "The mechanism of the hantzsch pyridine synthesis: A study by 15N and 13C NMR spectroscopy". Tetrahedron. 42 (20): 5729–5738. doi:10.1016/S0040-4020(01)88178-3.
- ^ Kumar, Atul (5 August 2008). "Efficient Synthesis of Hantzsch Esters and Polyhydroquinoline Derivatives in Aqueous Micelles". Chemlnform. 39 (32). doi:10.1002/chin.200832145.
- ^ Mashraqui, Sabir H. (1998). "Bismuth Nitrate Pentahydrate: A Convenient Reagent for the Oxidation of Hantzsch 1,4-Dihydropyridines". Synthesis (5): 713–714.
- ^ a b c d Yadav, Jhillu S. (2000). "Aromatization of Hantzsch 1,4-Dihydropyridines with I2-MeOH". Synthesis. 11: 1532–1534. doi:10.1055/s-2000-7613.
- ^ a b Ko, Kwang-Youn (1999). "Aromatization of Hantzsch 1,4-Dihydropyridines with Magtrieve". Tetrahedron Letters. 40: 3207–3208. doi:10.1016/S0040-4039(99)00467-0.
- ^ a b Liao, Xiali (2010). "Oxidative aromatization of Hantzsch 1,4-dihydropyridines by sodium chlorite". Tetrahedron Letters. 51: 3859–3861. doi:10.1016/j/tetlet.2010.05.091.
- ^ Wei, Xiaojing (2014). "Metal-Free-Mediated Oxidation Aromatization of 1,4-Dihydropyridines to Pyridines Using Visible Light and Air". Chinese Journal of Chemistry. 32: 1245–1250. doi:10.1002/cjoc.201400521.
- ^ Jassem, Ahmed Majeed; Almashal, Faeza Abdul Kareem; Mohammed, Mohammed Qasim; Jabir, Hadi Abdal Samad (7 February 2020). "A catalytic and green method for one-pot synthesis of new Hantzsch 1,4-dihydropyridines". SN Applied Sciences (2). doi:10.1007/s42452-020-2165-x.
- ^ Sharma, M.G; Rajani, D.P.; Patel, H.M. (14 June 2017). "Green approach for synthesis of bioactive Hantzsch 1,4-dihydropyridine derivatives based on thiophene moiety via multicomponent reaction". Royal Society Open Science. 4 (6). doi:10.1098/rsos.170006.