| County | Location | mi[1] | km | Old exit | New exit | Destinations | Notes |
|---|---|---|---|---|---|---|---|
| Hillsborough | Tampa | 0.000 | 0.000 | — | — |   I-275 (SR 93) – St. Petersburg, Ocala, Tampa International Airport, Downtown Tampa | |
| 1.154 | 1.857 | 1 | 1 |     SR 585 (21st Street / 22nd Street) to US 41 Bus. – Cruise Ships | |||
| 1.76[2] | 2.83 | — | 2 |  SR 618 (Selmon Expressway) – Brandon, Port of Tampa | I-4–Selmon Expressway Connector; access to or from SR 618 only in the same direction; left exit, left entrance westbound | ||
| 2.463[2] | 3.964 | 2 | 2 |  SR 569 (40th Street) | Former interchange | ||
| 3.266 | 5.256 | 3 | 3 | US 41 (50th Street) / Columbus Drive / E 14th Avenue | Eastbound left exit and westbound left entrance; eastbound right entrance and westbound left entrance | ||
| | 4.706 | 7.574 | 4 | 5 |  SR 574 (E Martin Luther King Jr. Boulevard) | ||
| | 5.573 | 8.969 | 5 | 6 | Orient Road | Eastbound exit and westbound entrance | |
| | 6.683 | 10.755 | 6 | 7 |   US 92 (Hillsborough Avenue / SR 600) / US 301 (SR 41) – Riverview, Zephyrhills, Busch Gardens | ||
| | 8.610 | 13.856 | 7 | 9 |  I-75 (SR 93A) – Ocala, Naples | ||
| Mango | 10.142 | 16.322 | 8 | 10 |  CR 579 – Mango, Thonotosassa | ||
| | 13.876 | 22.331 | 9 | 14 | McIntosh Road | ||
| | 17.434 | 28.057 | 10 | 17 | Branch Forbes Road | Serves Dinosaur World | |
| Plant City | 19.518 | 31.411 | 11 | 19 |  SR 566 (Thonotosassa Road) | ||
| 21.280 | 34.247 | 13 | 21 |   SR 39A (Alexander Street) / SR 39 (Buchman Highway) | Alexander Street was originally exit 12, but was combined with 13 | ||
| 22.596 | 36.365 | 14 | 22 |  SR 533 (N Park Road) | |||
| Hillsborough–Polk county line | Lakeland | 25.563 | 41.140 | 15 | 25 | County Line Road | |
| Polk | 26.530 | 42.696 | 15A | 27 |   SR 570 east (Polk Parkway) – Lakeland, Winter Haven, Bartow | ||
| 28.365 | 45.649 | 16 | 28 |  SR 546 (W Memorial Boulevard) to US 92 – Lakeland | |||
| 30.675 | 49.367 | 17 | 31 |     SR 539 south (Kathleen Road) / CR 35A north (Kathleen Road) – Kathleen, Lakeland | |||
| 32.003 | 51.504 | 18 | 32 |   US 98 (SR 700) / SR 35 – Lakeland, Dade City | |||
| 33.440 | 53.816 | 19 | 33 |   SR 33 (Lakeland Hills Boulevard) / CR 582 (N Socrum Loop Road) – Lakeland | |||
| 37.894 | 60.984 | 20 | 38 | SR 33 (Commonwealth Avenue / Lakeland Hills Boulevard) | |||
| 41.223 | 66.342 | 20A | 41 |  SR 570 west (Polk Parkway) – Auburndale, Lakeland | |||
| | 43.981 | 70.781 | 21 | 44 |  SR 559 – Polk City, Auburndale | ||
| | 47.982 | 77.220 | 22 | 48 |  CR 557 (Old Grade Road) – Lake Alfred, Winter Haven | ||
| | 54.733 | 88.084 | 23 | 55 |  US 27 (SR 25) – Haines City, Clermont | ||
| Osceola | | 57.723 | 92.896 | 24 | 58 |  CR 532 (Osceola Polk Line Road) – Poinciana, Kissimmee | |
| | 59.663 | 96.018 | — | 60 |   SR 429 north (Daniel Webster Western Beltway) – Apopka | ||
| Celebration | 61.781 | 99.427 | 24C-D-E | 62 |  SR 417 north (Central Florida GreeneWay) / World Drive – Disney World, Celebration, International Airport, Sanford | ||
| | 64.165 | 103.264 | 25A-B | 64 |  US 192 (SR 530 / W Irlo Bronson Memorial Highway) – Kissimmee, Magic Kingdom | ||
| | 65.322 | 105.126 | 26C–D | 65 |  CR 522 (W Osceola Parkway) to SR 417 (Central Florida GreeneWay) – Animal Kingdom, Wide World of Sports | ||
| Orange | | 66.565 | 107.126 | 26A-B | 67 |  SR 536 east (World Center Drive) to SR 417 (Central Florida GreeneWay) / Epcot Center Drive – Epcot, Downtown Disney | |
| Lake Buena Vista | 68.107 | 109.608 | 27 | 68 |   SR 535 south (Kissimmee Vineland Road) / CR 535 north (Kissimmee Vineland Road) – Kissimmee, Lake Buena Vista | ||
| | 70.983 | 114.236 | 27A | 71 | Central Florida Parkway – SeaWorld | Eastbound exit and westbound entrance | |
| | 71.744 | 115.461 | 28 | 72 |  SR 528 east – International Airport, Cape Canaveral | ||
| | 73.732 | 118.660 | 29A | 74A |  SR 482 (W Sand Lake Road) / International Drive | ||
| Orlando | 75.246 | 121.097 | 29B | 74B | Adventure Way – Universal | Westbound exit and entrance | |
| 75.246 | 121.097 | — | 75A | Universal Boulevard to International Drive – Universal | Eastbound exit and entrance | ||
| 75.046 | 120.775 | 30 | 75B |  SR 435 (S Kirkman Road) / International Drive | No south/east or north/east access | ||
| 76.359 | 122.888 | 31 | 77 |  Florida's Turnpike (SR 91) – Miami, Ocala | |||
| 77.760 | 125.143 | 31A | 78 | Conroy Road – The Mall at Millenia, Holy Land Experience | |||
| 79.147 | 127.375 | 32 | 79 |  CR 423 (John Young Parkway) | |||
| 80.474 | 129.510 | 33 | 80 |   US 17 / US 92 / US 441 (Orange Blossom Trail / SR 500 / SR 600) | Signed as exits 80A (south/west) and 80B (north/east) eastbound; previously signed as exits 33A and 33B accordingly; westbound is a left exit, signed as exit 80 and only providing south/west access | ||
| 81.004 | 130.363 | 34 | 81A | To US 17 / US 92 / US 441 / Michigan Street | Westbound exit and eastbound entrance | ||
| 81.469 | 131.112 | 35 | 81BC | Kaley Avenue | Signed as exits 81B (east) and 81C (west) westbound | ||
| 82.116 | 132.153 | 36 | 82A |  SR 408 (East–West Expressway) | |||
| 82.24[2] | 132.35 | 37 | 82B | Gore Street | former westbound-only interchange; westbound entrance remains | ||
| 82.636 | 132.990 | 38 | 82B | Anderson Street / South Street to Amway Center, History Center; | Eastbound exit to South Street; westbound exit to Anderson Street; eastbound entrances from South Street and Anderson Street; westbound entrance from South Street; formerly signed as exit 82C | ||
| 82.78[2] | 133.22 | 39 | 83 | South Street | Closed interchange; westbound left exit | ||
| 83.30[2] | 134.06 | 40 | 83A |  SR 526 (Robinson Street) | Former interchange; eastbound exit and westbound entrance | ||
| 83.792 | 134.850 | 41 | 83A |  US 17 / US 92 / SR 50 (Colonial Drive / SR 600) / Amelia Street – Bob Carr | Signed as exit 84 westbound; formerly signed as exit 83B eastbound | ||
| 84.279 | 135.634 | 42 | 84 | Ivanhoe Boulevard | Westbound ramp serves US 17, US 94, and SR 50 | ||
| 85.135 | 137.012 | 43 | 85 |  SR 438 (Princeton Street) | |||
| 85.890 | 138.227 | 44 | 86 | Par Street | Eastbound exit and westbound entrance | ||
| Winter Park | 86.789 | 139.673 | 45 | 87 |  SR 426 (Fairbanks Avenue) | ||
| 87.767 | 141.247 | 46 | 88 |  SR 423 (Lee Road) | |||
| Maitland | 89.491 | 144.022 | 47 | 90 |  SR 414 (Maitland Boulevard) | Signed as 90A (east) and 90B (west) eastbound | |
| Seminole | Altamonte Springs | 91.631 | 147.466 | 48 | 92 |  SR 436 – Altamonte Springs, Apopka | |
| Longwood | 93.613 | 150.656 | 49 | 94 |  SR 434 – Longwood, Winter Springs | ||
| Lake Mary | 98.400 | 158.359 | 50 | 98 | Lake Mary Boulevard – Lake Mary, Heathrow | ||
| 100.628 | 161.945 | 51A | 101A |  CR 46A – Sanford, Heathrow | |||
| Sanford | 101.366 | 163.133 | — | 101B | SR 417 south – International Airports (Sanford, Orlando), Sanford | ||
| 102.505 | 164.966 | 51 | 101C |  SR 46 – Mount Dora, Sanford Historic District | |||
| | 103.997 | 167.367 | 52 | 104 | US 17 / US 92 (SR 15 / SR 600) – Sanford | ||
| Volusia | Deltona–DeBary city line | 107.821 | 173.521 | 53 | 108 |  CR 4162 (Dirksen Drive / Debary Avenue) – Debary, Deltona | |
| Deltona–Orange City city line | 110.636 | 178.051 | 53C | 111 |  CR 4146 (Saxon Boulevard) – Deltona, Orange City | Signed as exits 111A (Deltona) and 111B (Orange City); previously signed as exits 53CA and 53CB accordingly | |
| Deltona–DeLand city line | 113.783 | 183.116 | 54 | 114 |  SR 472 – Deltona, DeLand | ||
| Lake Helen | 115.898 | 186.520 | 55 | 116 |  CR 4116 (Orange Camp Road / W Main Street) – DeLand, Lake Helen Historic District | ||
| DeLand | 118.456 | 190.636 | 56 | 118 |  SR 44 (E New York Avenue) – New Smyrna Beach, DeLand Historic District | Signed as exits 118A (east) and 118B (west) | |
| Daytona Beach | 129.131 | 207.816 | 57 | 129 | US 92 east (SR 600) – Daytona Beach | Eastbound exit and westbound entrance | |
| 131.987 | 212.412 | — | — |  I-95 (SR 9) – Daytona Beach, Jacksonville, Miami | |||
| 132.298 | 212.913 | 58 | 132 |  SR 400 east – South Daytona | |||
1.000 mi = 1.609 km; 1.000 km = 0.621 mi
| |||||||
- ^ Staff (August 4, 2010). "Florida Department of Transportation Interchange Report" (PDF). Florida Department of Transportation. pp. 2–3. Retrieved November 2, 2010.
- ^ a b c d e "Straight Line Diagrams". Florida Department of Transportation. Retrieved March 8, 2014.