Eyre was born in New York City, New York, the son of Dorothy Pelline (née Acton) and Edward Joseph Eyre, a banker. He was sent to a public school in England at the age of twelve, and has been based in the country ever since.
Although offered a place at the Royal Academy of Dramatic Art at the age of 18, he studied acting in Paris. His career in the theatre includes work with the Old Vic (his professional debut), the Royal Shakespeare Company, the Royal National Theatre, and The Old Vic Theatre Company under the direction of Kevin Spacey.
- Having a Wild Weekend (1965) - Art Director (uncredited)
- Julius Caesar (1970) - Cinna the Poet
- The Rivals of Sherlock Holmes (1971, TV Series) - George Fitzwilliam
- The Pied Piper (1972, by Jacques Demy) - Priest
- Mahler (1974, by Ken Russell) - Otto Mahler
- Hedda (1975) - Jørgen Tesman
- Luna (1979, by Bernardo Bertolucci) - Edward
- Dragonslayer (1981) - Casiodorus Rex
- Maurice (1987, by James Ivory) - Rev. Borenius
- Just Ask for Diamond (1988) - Gott
- Mountains of the Moon (1990) - Norton Shaw
- Let Him Have It (1991) - Humphreys
- Orlando (1992) - Pope
- The Remains of the Day (1993, by James Ivory) - Lord Halifax
- Princess Caraboo (1994) - Lord Apthorpe
- Surviving Picasso (1996, by James Ivory) - Sabartes
- The Tango Lesson (1997, by Sally Potter) - English Tango Fan
- Dangerous Beauty (1998) - The Doge
- Merlin (1998, TV Mini-Series) - Chief Physician
- Friends (1998, TV Series) - the registrar
- Midsomer Murders (1999, TV Series) - Leonard Pike (in S2:E2 “Strangler’s Wood”)
- Alice in Wonderland (1999, TV Movie) - Frogface Footman
- The Golden Bowl (2000, by James Ivory) - A.R. Jarvis, Shopkeeper
- From Hell (2001) - Lord Hallsham
- The Affair of the Necklace (2001) - Monsieur Bassenge
- The Situation (2006) - U.S. Ambassador
- Shadows in the Sun (2009) - Jonathan / 'Prospero'
- Peter Eyre profile, filmreference.com; accessed 5 March 2016.
- Koenig, Rhoda (9 February 2002). "Peter Eyre: Natural Eyre to the role". The Independent. London, UK. Retrieved 16 October 2009.
- Rock, Malcolm (7 May 2007). "20 Questions With... Peter Eyre". London: Whatsonstage.com. Retrieved 16 October 2009.
- "The Situation" (PDF) (Press release). Shadow Distribution. Retrieved 16 October 2009.
- Kevin Quarmby blog: Interview with Peter Eyre, Richard II, Old Vic Theatre, London, September 2005; accessed 5 March 2016.