Hydroxyisourate hydrolase

In enzymology, a hydroxyisourate hydrolase (EC 3.5.2.17) is an enzyme that catalyzes the chemical reaction

Hydroxyisourate hydrolase
Identifiers
EC no.3.5.2.17
Databases
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BRENDABRENDA entry
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KEGGKEGG entry
MetaCycmetabolic pathway
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PDB structuresRCSB PDB PDBe PDBsum
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NCBIproteins
5-hydroxyisourate + H2O 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate

Thus, the two substrates of this enzyme are 5-hydroxyisourate and H2O, whereas its product is 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is 5-hydroxyisourate amidohydrolase. Other names in common use include HIUHase, and 5-hydroxyisourate hydrolase. This enzyme participates in purine metabolism.

Structural studies

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As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2H1X and 2H6U.

See also

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References

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  • Raychaudhuri A, Tipton PA (2003). "A familiar motif in a new context: the catalytic mechanism of hydroxyisourate hydrolase". Biochemistry. 42 (22): 6848–52. doi:10.1021/bi034137o. PMID 12779339.
  • Raychaudhuri A, Tipton PA (2002). "Cloning and expression of the gene for soybean hydroxyisourate hydrolase. Localization and implications for function and mechanism". Plant Physiol. 130 (4): 2061–8. doi:10.1104/pp.011049. PMC 166717. PMID 12481089.
  • Sarma AD, Serfozo P, Kahn K, Tipton PA (1999). "Identification and purification of hydroxyisourate hydrolase, a novel ureide-metabolizing enzyme". J. Biol. Chem. 274 (48): 33863–5. doi:10.1074/jbc.274.48.33863. PMID 10567345.