Double-stranded uracil-DNA glycosylase (EC 3.2.2.28, Mug, double-strand uracil-DNA glycosylase, Dug, dsUDG, double-stranded DNA specific UDG, dsDNA specific UDG, UdgB, G:T/U mismatch-specific DNA glycosylase, UDG) is an enzyme with systematic name uracil-double-stranded DNA deoxyribohydrolase (uracil-releasing).[1][2][3] This enzyme catalyses a specific chemical reaction: it hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free uracil.
| Double-stranded uracil-DNA glycosylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.2.2.28 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
This enzyme is not active on DNA containing a T/G mispair or single-stranded DNA.
References
edit- ^ Barrett TE, Schärer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH (December 1999). "Crystal structure of a thwarted mismatch glycosylase DNA repair complex". The EMBO Journal. 18 (23): 6599–609. doi:10.1093/emboj/18.23.6599. PMC 1171723. PMID 10581234.
- ^ Sung JS, Mosbaugh DW (August 2000). "Escherichia coli double-strand uracil-DNA glycosylase: involvement in uracil-mediated DNA base excision repair and stimulation of activity by endonuclease IV". Biochemistry. 39 (33): 10224–35. doi:10.1021/bi0007066. PMID 10956012.
- ^ Mokkapati SK, Fernández de Henestrosa AR, Bhagwat AS (September 2001). "Escherichia coli DNA glycosylase Mug: a growth-regulated enzyme required for mutation avoidance in stationary-phase cells". Molecular Microbiology. 41 (5): 1101–11. doi:10.1046/j.1365-2958.2001.02559.x. PMID 11555290.
External links
edit- Double-stranded+uracil-DNA+glycosylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
