In enzymology, a columbamine oxidase (EC 1.21.3.2) is an enzyme that catalyzes the chemical reaction

columbamine oxidase
Identifiers
EC no.1.21.3.2
CAS no.95329-18-3
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins
2 columbamine + O2 2 berberine + 2 H2O

Thus, the two substrates of this enzyme are columbamine and O2, whereas its two products are berberine and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on X-H and Y-H to form an X-Y bond with oxygen as acceptor. The systematic name of this enzyme class is columbamine:oxygen oxidoreductase (cyclizing). This enzyme is also called berberine synthase. This enzyme participates in alkaloid biosynthesis i. It employs one cofactor, iron.

References

edit
  • Rueffer M, Zenk MH (1985). "Berberine synthesis, the methylenedioxy group forming enzyme in berberine synthesis". Tetrahedron Lett. 26 (2): 201–202. doi:10.1016/S0040-4039(00)61879-8.