Wikipedia

Alan Christoffels

Alan Christoffels is a bioinformatics scientist, academic, and an author. He is Professor of Bioinformatics, and the director of the South African National Bioinformatics Institute at the University of the Western Cape.[1] He has been serving as a senior advisor to the Africa Centres for Disease Control and Prevention Pathogen genomics & Partnerships and DSI/NRF Research Chair in Bioinformatics and Public Health Genomics.[2]

Alan Christoffels
Born
Kensington, Cape Town, South Africa
Occupation(s)Bioinformatics scientist, academic, and an author
Academic background
EducationBSc. (1992), BSc. (1993), MSc. (1995), Ph.D. (2001)
Alma mater
ThesisGeneration of a human gene index and its application to disease candidacy (2001)
Academic work
InstitutionsSouth African National Bioinformatics Institute, University of the Western Cape

Christoffels’ primary contributions and research work are in the areas of host-pathogen interaction, genome evolution, pathogen genomics, and biobank LIMS.[3]

Christoffels is a founding member of Global Emerging Pathogens Consortium,[4] elected member of the Academy of Science of South Africa,[5] and President of the African Society for Bioinformatics and Computational Biology from 2020 until 2022. He was elected as a fellow of the Royal Society of South Africa in 2022.[6]

Education edit

Christoffels graduated with BSc. in Microbiology and Biochemistry in 1992 and BSc (Hons.) in Pharmacology from the University of Cape Town in 1993. Between 1995 and 1997, he enrolled at the Stellenbosch University for a MSc. in Genetics.[7] His research centered upon identification of novel markers in the fine mapping of a locally prevalent cardiac disease (PFHB1).[8] He proceeded with a Ph.D. in bioinformatics at the South African National Bioinformatics Institute University of the Western Cape. His thesis was titled, "Generation of a human gene index and its application to disease candidacy".[9]

Career edit

Christoffels started his academic career in 1994 as a Genetics Technician in medical Biochemistry at the Stellenbosch University. Between 2001 and 2004, he held the postdoctoral fellowship at the Institute of Molecular & Cell Biology in Singapore. Followed by this appointment, he served as an Adjunct Assistant Professor at the Nanyang Technological University based in Singapore. Later on, he held the appointment of associate professor from 2007 to 2012 in the South African National Bioinformatics Institute at the University of the Western Cape.[10] He was promoted to the position of Professor in 2013 and currently holds this appointment.[11]

From 2009, Christoffels serves as the Director of South African National Bioinformatics Institute at the University of the Western Cape.[12] For the next five years, he served on the board of directors for International Society for Computational Biology (ISCB). He has been holding the appointment of SA Medical Research Council Unit Director at the Bioinformatics Capacity Development Unit since 2012. Later on, he held a brief appointment as vice president for the South African Society for Bioinformatics.[13] He was appointed as President of African Society of Bioinformatics & Computational Biology in 2020 for a two-year term.[14]

Followed by stakeholder meeting convened by Bill & Melinda Gates Foundation, Christoffels and his international partners launched a global initiative called Public Health Alliance for Genomic Epidemiology[15] in October 2019 at the GrandChallenges meeting in Ethiopia. He serves as the principal investigator of this initiative. The research prototypes generated in the lab are integrated into the working groups within the alliance.[16]

Research edit

Christoffels has more than 400 publications under his name.[3] His research spans host-pathogen interaction, pathogen genomics, genome evolution with a particular focus on the genome annotation and sequence data analysis.[17]

Public health bioinformatics edit

Christoffels and a team of scientists at his lab based in South African National Bioinformatics Institute presented the first genome sequence of SARS-CoV-2, the virus that causes COVID-19, found in South Africa.[18] His recent work features conducting research on scaling up disease surveillance systems on the African continent.[19][20]

Christoffels has developed technologies for application of bioinformatics in public health. He has developed an analysis platform for Tuberculosis sequencing data called COMBAT-TB. This tool can be deployed in resource limited settings.[21]

Biobank LIMS edit

With a group of researchers, Christoffels developed a management system for biobanking, Laboratory Information Management System (LIMS) at the South African National Bioinformatics Institute.[22] The purpose of the management system is to collect, store, process and, manage the bio samples appropriately. This human biobanking system is based upon the Plone web-content management framework.[23]

Disease vectors edit

Christoffels has focused his genomics research on the disease vectors. In 2016, he described the non-coding RNA (miRNA) in Anopheles funestus, and postulated roles of these small genes in understanding of parasitic control by blood-sucking mosquito.[24] He co-led a tsetse fly genome project between 2008 and 2014 where him and his students studied the way a fly regulates its own immune system and protects itself against iron toxicity. He has subsequently used his analytical methods to describe non-coding RNA regulation in the Black soldier fly.[25]

Genome sequencing and annotation projects (2004-2016) edit

Christoffels' earlier research focused on sequencing, annotating, and analyzing genomes. He analyzed the genome assembly and annotation of the Fugu rubripes. With an over 95% sequenced coverage, it is indicated that the 80% of the assembly is in multigene-sized scaffolds. The study reported that in the genome, the repetitive DNA occupy less than one-sixth of the sequence, whereas the gene loci occupy about one-third of the genome. It also highlighted that protein evolution since 450 million years, although the three-quarters of human proteins had either diverged from or did not have pufferfish homologs. Given a significant gene order scrambling, the conserved linkages between Fugu rubripes and humans show the preservation of segments of chromosomes associated with the common vertebrate ancestor.[26] A systematic comparison was carried out between the draft genome sequence of Fugu and humans for identifying paralogous chromosomal regions in the Fugu. The duplicate genes were indicated in the Fugu after using phylogenetic analyses of the Fugu, human and invertebrate sequences. The analysis determined evidence for 425 fish-specific duplicate genes in the Fugu and indicated that at least 6.6% of the genome is represented by fish-specific paralogons. The study also strongly suggests a whole-genome duplication during the ray-finned fish evolution, which may also have occurred before the origin of teleosts.[27]

In 2013, Christoffels reported the analysis of the African coelacanth genome.[28] The genome sequence provided genetic changes which address the adaption from the aquatic environment to land.[29] The study was conducted for obtaining insights into the tetrapod evolution. The phylogenomic analysis highlighted the closest living association of lungfish with the tetrapod instead of coelacanth. The protein-coding genes of Coelacanth also revealed a slow evolution than that of tetrapod.[30]

In 2013, Christoffels completed the annotation of the coelacanth genome in his lab. Later on, the analysis on the taste and odorant receptors in the coelacanth was carried out and it was demonstrated that the repertoire of GPCR chemosensory receptors (CRs) of the Coelacanth supports its intermediary position.[31]

The Tsetse project spanned nearly 10 years.[32][33] Christoffels was part of the executive team who managed the genome project,[34] and led the scientific analyses by supervising the Ph.D. students who analyzed different regions of the genome in detail as it pertains to innate protection against the pathogen (Trypanosome),[35] Trypanosomatid SNAREs comparison,[36] chemical signaling to find a host,[37] and promoter architecture in Tsetse.[38]

Followed by his sequencing projects in coelacanth and Tsetse genomes, Christoffels co-lead the genome assembly and annotation of the Asian Seabass in 2014.[39] The group combined for the first-time long reads (PacBio) and short reads (illumina) sequencing data to assemble a non-model eukaryotic genome at that time. He also led the genome assembly and annotation teams in South Africa and Singapore.[40] He had defined the parameters for non-model organisms as well.[41]

Community engagement edit

He has authored three books, titled How to be a Health Activist: Teacher’s Guide, How to Be a Health Activist: A Life Orientation Workbook, and How to Be a Health Activist: A life skills workbook for grades 7-9 learners.

Throughout his research career, Christoffels has added a community engagement dimension to his work. He has achieved this initially with integrating awareness of Tuberculosis into school curricula activities. Later he developed audio books for communicating the value of biobanks in multiple languages.[42]

Awards and honors edit

  • 2014 – South African Medical Research Council Silver Award
  • 2015 – Hamilton Naki Award, National Research Foundation [43]
  • 2016 – Fulbright Visiting Scholar Award [44]
  • 2017 – Outstanding Alumnus Award, University of the Western Cape

Bibliography edit

Books edit

  • How to be a Health Activist: Teacher’s Guide (2013) ISBN 978-0-86808-745-0
  • How to Be a Health Activist: A Life Orientation Workbook (2014) ISBN 978-0-86808-748-1
  • How to Be a Health Activist: A life skills workbook for grades 7-9 learners (2014) ISBN 978-0-620-51650-1

Selected articles edit

  • Aparicio, S., Chapman, J., Stupka, E., Putnam, N., Chia, J. M., Dehal, P., ... & Brenner, S. (2002). Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes. Science, 297(5585), 1301–1310.
  • Christoffels, A., Koh, E. G., Chia, J. M., Brenner, S., Aparicio, S., & Venkatesh, B. (2004). Fugu genome analysis provides evidence for a whole-genome duplication early during the evolution of ray-finned fishes. Molecular biology and evolution, 21(6), 1146–1151.
  • Amemiya, C. T., Alföldi, J., Lee, A. P., Fan, S., Philippe, H., MacCallum, I., ... & Lindblad-Toh, K. (2013). The African coelacanth genome provides insights into tetrapod evolution. Nature, 496(7445), 311–316.
  • Vij, S., Kuhl, H., Kuznetsova, I. S., Komissarov, A., Yurchenko, A. A., Van Heusden, P., ... & Orbán, L. (2016). Chromosomal-level assembly of the Asian seabass genome using long sequence reads and multi-layered scaffolding. PLoS genetics, 12(4), e1005954.
  • Allam, M., Spillings, B. L., Abdalla, H., Mapiye, D., Koekemoer, L. L., & Christoffels, A. (2016). Identification and characterization of microRNAs expressed in the African malaria vector Anopheles funestus life stages using high throughput sequencing. Malaria Journal, 15(1), 1–15.
  • Choudhury, A., Ramsay, M., Hazelhurst, S., Aron, S., Bardien, S., Botha, G., ... & Pepper, M. S. (2017). Whole-genome sequencing for an enhanced understanding of genetic variation among South Africans. Nature communications, 8(1), 1–12.
  • Lose, T., van Heusden, P., & Christoffels, A. (2020). COMBAT-TB-NeoDB: fostering tuberculosis research through integrative analysis using graph database technologies. Bioinformatics, 36(3), 982–983.
  • van Heusden, P., Mashologu, Z., Lose, T., Warren, R., & Christoffels, A. (2022). The COMBAT-TB Workbench: Making Powerful Mycobacterium tuberculosis Bioinformatics Accessible. Msphere, 7(1), e00991-21.

References edit

  1. ^ "Professor Alan Christoffels, Director SANBI, visits DELGEME". delgeme.org.
  2. ^ "Prof. Alan Christoffels".
  3. ^ a b "Alan Christoffels". scholar.google.com.
  4. ^ "Alan Christoffels, PhD « ICREID".
  5. ^ "Members – ASSAf".
  6. ^ "UWC Professor elected to the Royal Society of South Africa".
  7. ^ "Prof. Alan Christoffels". repository.uwc.ac.za.
  8. ^ Christoffels, Alan Gilbert (August 29, 1997). Identification of novel CA repeat markers for use in fine mapping of the PFHBI gene and screening of the HRC candidate gene (Thesis) – via scholar.sun.ac.za.
  9. ^ Christoffels, Alan (August 29, 2001). Generation of a human gene index and its application to disease candidacy (Thesis). University of the Western Cape – via etd.uwc.ac.za.
  10. ^ "H3Africa Conference participant details1" (PDF).
  11. ^ "Prof Alan Christoffels".
  12. ^ "Prof. Alan Christoffels – Inter-University Institute for Data Intensive Astronomy". 11 February 2019.
  13. ^ "SANBI Director, Prof Alan Christoffels appointed as a Senior Advisor at the Africa CDC". South African National Bioinformatics Institute.
  14. ^ "Africa". www.iscb.org.
  15. ^ "Public Health Alliance for Genomic Epidemiology".
  16. ^ "Prof Alan Christoffels – SANBI – South African National Bioinformatics Institute".
  17. ^ "Research Gate Profile".
  18. ^ Spotlight, Kathryn Cleary for (April 14, 2020). "The Genetic Fingerprint: How scientists unlocked SA's SARS-CoV-2 virus". Daily Maverick.
  19. ^ "How genome sequencing helps in tackling COVID-19 variants -WHO". October 2, 2021.
  20. ^ Tessema, Sofonias K; Inzaule, Seth C; Christoffels, Alan; Kebede, Yenew; de Oliveira, Tulio; Ouma, Ahmed E Ogwell; Happi, Christian T; Nkengasong, John N (October 1, 2020). "Accelerating genomics-based surveillance for COVID-19 response in Africa". The Lancet Microbe. 1 (6): e227–e228. doi:10.1016/S2666-5247(20)30117-8. PMC 7434434. PMID 32838350.
  21. ^ Van Heusden, P.; Mashologu, Z.; Lose, T.; Warren, R.; Christoffels, A. (2022). "The COMBAT-TB Workbench: Making Powerful Mycobacterium tuberculosis Bioinformatics Accessible". mSphere. 7 (1): e0099121. doi:10.1128/msphere.00991-21. hdl:10566/7914. PMC 8827006. PMID 35138128.
  22. ^ "BAfrica".
  23. ^ Hiller, Dr Reinhard (October 19, 2017). "South Africa's Centre for Proteomic & Genomic Research (CPGR) to implement Baobab LIMS to boost lab quality control and operational performance". CPGR.
  24. ^ Allam, Mushal; Spillings, Belinda L.; Abdalla, Hiba; Mapiye, Darlington; Koekemoer, Lizette L.; Christoffels, Alan (November 8, 2016). "Identification and characterization of microRNAs expressed in the African malaria vector Anopheles funestus life stages using high throughput sequencing". Malaria Journal. 15 (1): 542. doi:10.1186/s12936-016-1591-0. PMC 5101901. PMID 27825380.
  25. ^ DeRaedt, Sarah; Bierman, Anandi; Heusden, Peter van; Richards, Cameron; Christoffels, Alan (March 17, 2022). "microRNA profile of Hermetia illucens (black soldier fly) and its implications on mass rearing". PLOS ONE. 17 (3): e0265492. Bibcode:2022PLoSO..1765492D. doi:10.1371/journal.pone.0265492. PMC 8929568. PMID 35298540.
  26. ^ Aparicio, Samuel; Chapman, Jarrod; Stupka, Elia; Putnam, Nik; Chia, Jer-ming (August 23, 2002). "Whole-Genome Shotgun Assembly and Analysis of the Genome of Fugu rubripes". Science. 297 (5585): 1301–1310. Bibcode:2002Sci...297.1301A. doi:10.1126/science.1072104. PMID 12142439. S2CID 10310355.
  27. ^ "Fugu Genome Analysis Provides Evidence for a Whole-Genome Duplication Early During the Evolution of Ray-Finned Fishes".
  28. ^ "The people's fish: Coelacanth genes get sequenced". May 10, 2013.
  29. ^ "Coelacanth genome informs land vertebrate evolution". ScienceDaily.
  30. ^ Amemiya, Chris T.; Alföldi, Jessica; Lee, Alison P.; Fan, Shaohua; Philippe, Hervé (April 29, 2013). "The African coelacanth genome provides insights into tetrapod evolution". Nature. 496 (7445): 311–316. Bibcode:2013Natur.496..311A. doi:10.1038/nature12027. PMC 3633110. PMID 23598338.
  31. ^ Picone, Barbara; Hesse, Uljana; Panji, Sumir; Van Heusden, Peter; Jonas, Mario; Christoffels, Alan (September 29, 2014). "Taste and odorant receptors of the coelacanth--a gene repertoire in transition". Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution. 322 (6): 403–414. Bibcode:2014JEZB..322..403P. doi:10.1002/jez.b.22531. PMID 24106203 – via PubMed.
  32. ^ Christoffels, A.; Masiga, D.; Berriman, M.; Lehane, M.; Touré, Y.; Aksoy, S. (2014). "International Glossina Genome Initiative 2004–2014: A Driver for Post-Genomic Era Research on the African Continent - PMC". PLOS Neglected Tropical Diseases. 8 (8): e3024. doi:10.1371/journal.pntd.0003024. PMC 4140670. PMID 25144472.
  33. ^ "Tsetse Fly Genome". www.uwc.ac.za.
  34. ^ Butler, Declan (January 1, 2004). "African labs win major role in tsetse-fly genome project". Nature. 427 (6973): 384. doi:10.1038/427384b. PMID 14749791. S2CID 8642083.
  35. ^ Dashti, Zahra Jalali Sefid; Gamieldien, Junaid; Christoffels, Alan (August 8, 2016). "Computational characterization of Iron metabolism in the Tsetse disease vector, Glossina morsitans: IRE stem-loops". BMC Genomics. 17 (1): 561. doi:10.1186/s12864-016-2932-7. PMC 4977773. PMID 27503259.
  36. ^ Murungi, Edwin; Barlow, Lael D.; Venkatesh, Divya; Adung'a, Vincent O.; Dacks, Joel B.; Field, Mark C.; Christoffels, Alan (April 1, 2014). "A comparative analysis of trypanosomatid SNARE proteins". Parasitology International. 63 (2): 341–348. doi:10.1016/j.parint.2013.11.002. PMC 3979113. PMID 24269876.
  37. ^ Masiga, D.; Obiero, G.; Macharia, R.; Mireji, P.; Christoffels, A. (2014). "Chemosensory receptors in tsetse flies provide link between chemical and behavioural ecology - PMC". Trends in Parasitology. 30 (9): 426–428. doi:10.1016/j.pt.2014.06.007. PMC 4679831. PMID 25017128.
  38. ^ Mwangi, Sarah; Attardo, Geoffrey; Suzuki, Yutaka; Aksoy, Serap; Christoffels, Alan (September 22, 2015). "TSS seq based core promoter architecture in blood feeding Tsetse fly (Glossina morsitans morsitans) vector of Trypanosomiasis". BMC Genomics. 16 (1): 722. doi:10.1186/s12864-015-1921-6. PMC 4578606. PMID 26394619.
  39. ^ "SANBI – 2015 Annual report" (PDF).
  40. ^ Vij, Shubha; Kuhl, Heiner; Kuznetsova, Inna S.; Komissarov, Aleksey (April 15, 2016). "Chromosomal-Level Assembly of the Asian Seabass Genome Using Long Sequence Reads and Multi-layered Scaffolding". PLOS Genetics. 12 (4): e1005954. doi:10.1371/journal.pgen.1005954. PMC 4833346. PMID 27082250.
  41. ^ Mbandi, Stanley Kimbung; Hesse, Uljana; van Heusden, Peter; Christoffels, Alan (February 21, 2015). "Inferring bona fide transfrags in RNA-Seq derived-transcriptome assemblies of non-model organisms". BMC Bioinformatics. 16 (1): 58. doi:10.1186/s12859-015-0492-5. PMC 4344733. PMID 25880035.
  42. ^ Bedeker, A.; Anderson, D.; Lose, T.; Mgwatyu, Y.; Luus, R.; Blignaut, R.; Christoffels, A. (2019). "Understanding biobanking: An assessment of the public engagement speaking book intervention Biobanking and Me". South African Journal of Bioethics and Law. 12 (2): 87. doi:10.7196/SAJBL.2019.v12i2.691.
  43. ^ "SA's top researchers honoured". SAnews. August 28, 2015.
  44. ^ "Broad Institute | Fulbright Scholar Program". fulbrightscholars.org.
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