N-Acetylserotonin O-methyltransferase also known as ASMT is an enzyme that catalyzes the final reaction in melatonin biosynthesis, converting Normelatonin to melatonin. This reaction is embedded in the more general tryptophan metabolism pathway. The enzyme also catalyzes a second reaction in tryptophan metabolism: the conversion of 5-hydroxy-indoleacetate to 5-methoxy-indoleacetate.
|PDB structures||RCSB PDB PDBe PDBsum|
|Gene Ontology||AmiGO / QuickGO|
|, ASMTY, HIOMT, HIOMTY, acetylserotonin O-methyltransferase|
Structure and Gene LocationEdit
N-Acetylserotonin O-methyltransferase is an enzyme that is coded for by genes located on the pseudoautosomal region of the X and Y chromosome, and is most abundantly found in the pineal gland and retina of humans. Although the exact structure of N- Acetylserotonin O-methyltransferase has yet to be determined by X-Ray diffraction, the crystal structure of the Maf domain of human N-Acetylserotonin O-methyltransferase-like protein has been found.
Class of Enzyme and FunctionEdit
It catalyzes two reactions in the tryptophan metabolism pathway, and both can be traced back to serotonin. Serotonin has many fates in this pathway, and N- Acetylserotonin O-methyltransferase catalyzes reactions in two of these fates. The enzyme has been studied most for its catalysis of the final step of the pathway from serotonin to melatonin, but it also catalyzes one of the reactions in the many step process of serotonin → 5-Methoxy-indolacetate.
Synonyms of N- Acetylserotonin O-methyltransferase are Hydroxyindole O-methyltransferase (HIOMT), Acetylserotonin O-methyltransferase (ASMT), Acetylserotonin N-methyltransferase, Acetylserotonin methyltransferase (Y chromosome). The most commonly used synonym is Hydroxyindole O-methyltransferase (HIOMT).
N- Acetylserotonin O-methyltransferase is found in both prokaryotes and eukaryotes. It is found in the bacteria Rhodopirellula baltica and Chromobacterium violaceum. It is also found in the following eukaryotes: Gallus gallus (chicken), Bos taurus (cow), Homo sapiens (human), Macaca mulatta (rhesus monkey), and Rattus norvegicus (rat).
Amino Acid SequencesEdit
MCSQEGEGYSLLKEYANAFMVSQVLFAACELGVFELLAEALEPLDSAAVSSHLGSSPGD RAATEHLCVPEAAASRREGRKSCVCKHGARQHLPGERQPQVPAGHAAVRGQDRLRLLAP PGEAVREGRNQYLKAFGIPSEELFSAIYRSEDERLQFMQGLQDVWRLEGATVLAAFDLS PFPLICDLGGGSGALAKACVSLYPGCRAIVFDIPGVVQIAKRHFSASEDERISFHEGDF FKDALPEADLYILARVLHDWTDAKCSHLLQRVYRACRTGGGILVIESLLDTDGRGPLTT LLYSLNMLVQTEGRERTPGRSTARSVGPAASETCGDGGRGEPTMLSWPGNQACSV
For Homo sapiens (human) with 373 Amino Acids  the sequence is:
MGSSEDQAYRLLNDYANGFMVSQVLFAACELGVFDLLAEAPGPLDVAAVAAGVRASAHG TELLLDICVSLKLLKVETRGGKAFYRNTELSSDYLTTVSPTSQCSMLKYMGRTSYRCWG HLADAVREGRNQYLETFGVPAEELFTAIYRSEGERLQFMQALQEVWSVNGRSVLTAFDL SVFPLMCDLGGTRIKLETIILSKLSQGQKTKHRVFSLIGGAGALAKECMSLYPGCKITV FDIPEVVWTAKQHFSFQEEEQIDFQEGDFFKDPLPEADLYILARVLHDWADGKCSHLLE RIYHTCKPGGGILVIESLLDEDRRGPLLTQLYSLNMLVQTEGQERTPTHYHMLLSSAGF RDFQFKKTGAIYDAILARK
The human HOIMT gene is approximately 35 kb in length and contains 9-10 exons. The gene can be alternatively spliced to form at least three possible isoforms, although each of these isoforms has the same role in the biosynthesis of melatonin. It has also been found that the gene contains multiple promoter regions, an indication that multiple mechanisms of regulation exist.
Expression in immune cellsEdit
Recent studies found messenger RNA (mRNA) transcripts of the HOIMT gene in B lymphocytes, T helper lymphocytes, cytoxic T lymphocytes, and natural killer lymphocytes in humans. This finding, in conjunction with research on alternative splicing of the HOIMT hnRNA, suggests that Hydroxyindole O-methyltransferase (synonym for N- Acetylserotonin O-methyltransferase) plays a role in the human immune system, in addition to its endocrine and nervous system functions. In other words, the gene may be expressed in various isoforms in different cells of the body.
In the tryptophan metabolism pathway, N- Acetylserotonin O-methyltransferase catalyzes two separate reactions. The first reaction shown (Figure 2) is the reaction of N-acetyl-serotonin to N-acetyl-5-methoxy-tryptamine. S-adenosyl-L-methionine is used as a substrate and is converted to S-adenosyl-L-homocysteine.Figure 2: Reaction catalyzed by N- Acetylserotonin O-methyltransferase
Figure 3 is the same reaction as above, but the figure provides a clearer picture of how the reactant proceeds to product using N-Acetylserotonin O-methyltransferase in addition to the substrate.
Figure 3: Role of N- Acetylserotonin O-methyltransferase
The second reaction (Figure 4) catalyzed by N-Acetylserotonin O-methyltransferase in the tryptophan metabolism pathway is: S-Adenosyl-L-methionine + 5-Hydroxyindoleacetate ↔ S-Adenosyl-L-homocysteine + 5-Methoxyindoleacetate.
Figure 4: Second reaction catalyzed by N- Acetylserotonin O-methyltransferase
Figure 5 is a more general scheme of the reaction pathway from serotonin to melatonin. The number 188.8.131.52 refers to the Enzyme Commission Number (EC Number) for N- Acetylserotonin O-methyltransferase. These two steps are embedded in the highly involved tryptophan metabolism pathway.
Figure 5: Pathway serotonin → melatonin
There is evidence of high HIOMT gene expression in pineal parenchymal tumors (PPTs). This finding has led to the study of varying gene expression as a diagnostic marker for such tumors. Abnormally high levels of HIOMT in these glands could serve as an indication of the existence of PPTs in the brain.
Melatonin levels are used as a trait marker for mood disorders, meaning that abnormal levels of melatonin can be used in conjunction with other diagnostic criteria to determine whether a mood disorder (e.g. Seasonal affective disorder, bipolar disorder, or major depressive disorder) exists. Melatonin levels can also be used as a state marker, contributing to conclusions on the severity of a patient’s illness at a given point in time. Because studies have shown a direct correlation between the amount of hydroxyindole-O-methyltransferase in the pineal gland and the melatonin level, additional knowledge of HIOMT could provide valuable insight on the nature and onset of these impairing disorders.
High frequency polymorphism exists on the PAR region of the sex chromosomes, where the HIOMT gene is located. Linkage analysis of a diseased locus with high frequency polymorphism of this region could lead to vital information about the role of this gene in genetic disorders.
HIOMT as the limiting reagent in the melatonin biosynthetic pathway
There has been some controversy over the regulatory power of hydroxyindole-O-methyltransferase in the production of melatonin. In 2001, it was argued that another enzyme in the pathway, N-acetyl transferase (NAT) was the limiting reagent in the production of melatonin. Recent findings, however, have suggested that HIOMT, not NAT, is the limiting reagent, and a direct correlation between HIOMT expression and melatonin levels has been shown to exist.
- GRCh38: Ensembl release 89: ENSG00000196433 - Ensembl, May 2017
- "Human PubMed Reference:".
- Kanehisa M.; Goto S.; Hattori M.; Aoki-Kinoshita K.F.; Itoh M.; Kawashima S.; Katayama T.; Araki M.; Hirakawa M.; et al. (2006). "From genomics to chemical genomics: new developments in KEGG". Nucleic Acids Res. 34: D354–357. doi:10.1093/nar/gkj102. PMC 1347464. PMID 16381885. [See also comments in Thomson's website]
- Donohue SJ, Roseboom PH, Illnerova H, Weller JL, Klein DC (October 1993). "Human hydroxyindole-O-methyltransferase: presence of LINE-1 fragment in a cDNA clone and pineal mRNA". DNA Cell Biol. 12 (8): 715–27. doi:10.1089/dna.1993.12.715. PMID 8397829.
- Rodriguez IR, Mazuruk K, Schoen TJ, Chader GJ (December 1994). "Structural analysis of the human hydroxyindole-O-methyltransferase gene. Presence of two distinct promoters". J. Biol. Chem. 269 (50): 31969–77. PMID 7989373.
- Online Mendelian Inheritance in Man (OMIM) x-chromosomal ASMT -300015
- ; Min J, Wu H, Dombrovski L, Loppanu P, Weigelt J, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarve A, Plotnikov AM, Crystal Structure of Maf Domain of Human N- Acetylseratonin O-methyltransferase, Structural Genomics Consotrium (SGC) (2007)
- Enzyme 184.108.40.206 at KEGG Pathway Database.
- Pozo D, García-Mauriño S, Guerrero JM, Calvo JR (August 2004). "mRNA expression of nuclear receptor RZR/RORalpha, melatonin membrane receptor MT, and hydroxindole-O-methyltransferase in different populations of human immune cells". J. Pineal Res. 37 (1): 48–54. doi:10.1111/j.1600-079X.2004.00135.x. PMID 15230868.
- Caspi R, Foerster H, Fulcher CA, Hopkinson R, Ingraham J, Kaipa P, Krummenacker M, Paley S, Pick J, Rhee SY, Tissier C, Zhang P, Karp PD (January 2006). "MetaCyc: a multiorganism database of metabolic pathways and enzymes" (PDF). Nucleic Acids Res. 34 (Database issue): D511–6. doi:10.1093/nar/gkj128. PMC 1347490. PMID 16381923.
- Maltsev N, Glass E, Sulakhe D, Rodriguez A, Syed MH, Bompada T, Zhang Y, D'Souza M (January 2006). "PUMA2--grid-based high-throughput analysis of genomes and metabolic pathways". Nucleic Acids Res. 34 (Database issue): D369–72. doi:10.1093/nar/gkj095. PMC 1347457. PMID 16381888.
- Fèvre-Montange M, Champier J, Szathmari A, Wierinckx A, Mottolese C, Guyotat J, Figarella-Branger D, Jouvet A, Lachuer J (July 2006). "Microarray analysis reveals differential gene expression patterns in tumors of the pineal region". J. Neuropathol. Exp. Neurol. 65 (7): 675–84. doi:10.1097/01.jnen.0000225907.90052.e3. PMID 16825954.
- Srinivasan V, Smits M, Spence W, Lowe AD, Kayumov L, Pandi-Perumal SR, Parry B, Cardinali DP (2006). "Melatonin in mood disorders". World J. Biol. Psychiatry. 7 (3): 138–51. doi:10.1080/15622970600571822. PMID 16861139.
- Yi H, Donohue SJ, Klein DC, McBride OW (February 1993). "Localization of the hydroxyindole-O-methyltransferase gene to the pseudoautosomal region: implications for mapping of psychiatric disorders". Hum. Mol. Genet. 2 (2): 127–31. doi:10.1093/hmg/2.2.127. PMID 8098975.
- Djeridane Y, Touitou Y (April 2001). "Chronic diazepam administration differentially affects melatonin synthesis in rat pineal and Harderian glands". Psychopharmacology. 154 (4): 403–7. doi:10.1007/s002130000631. PMID 11349394.
- Reiter RJ, Tan DX, Terron MP, Flores LJ, Czarnocki Z (2007). "Melatonin and its metabolites: new findings regarding their production and their radical scavenging actions" (PDF). Acta Biochim. Pol. 54 (1): 1–9. PMID 17351668.
- Itoh MT, Hosaka T, Mimuro T, et al. (2003). "Gonadotropin-releasing hormone increases melatonin release in the pineal gland of the female rat in vitro". Horm. Metab. Res. 35 (3): 153–7. doi:10.1055/s-2003-39076. PMID 12734775.
- Ross MT, Grafham DV, Coffey AJ, et al. (2005). "The DNA sequence of the human X chromosome". Nature. 434 (7031): 325–37. doi:10.1038/nature03440. PMC 2665286. PMID 15772651.
- Fukuda T, Akiyama N, Ikegami M, et al. (2010). "Expression of hydroxyindole-O-methyltransferase enzyme in the human central nervous system and in pineal parenchymal cell tumors". J. Neuropathol. Exp. Neurol. 69 (5): 498–510. doi:10.1097/NEN.0b013e3181db7d3c. PMID 20418777.
- Donohue SJ, Roseboom PH, Illnerova H, et al. (1993). "Human hydroxyindole-O-methyltransferase: presence of LINE-1 fragment in a cDNA clone and pineal mRNA". DNA Cell Biol. 12 (8): 715–27. doi:10.1089/dna.1993.12.715. PMID 8397829.
- Toma C, Rossi M, Sousa I, et al. (2007). "Is ASMT a susceptibility gene for autism spectrum disorders? A replication study in European populations". Mol. Psychiatry. 12 (11): 977–9. doi:10.1038/sj.mp.4002069. PMID 17957233.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Jonsson L, Ljunggren E, Bremer A, et al. (2010). "Mutation screening of melatonin-related genes in patients with autism spectrum disorders". BMC Med. Genom. 3: 10. doi:10.1186/1755-8794-3-10. PMC 3020629. PMID 20377855.
- Holt R, Barnby G, Maestrini E, et al. (2010). "Linkage and candidate gene studies of autism spectrum disorders in European populations". European Journal of Human Genetics. 18 (9): HASH(0x24e5530). doi:10.1038/ejhg.2010.69. PMC 2987412. PMID 20442744.
- Gałecki P, Szemraj J, Bartosz G, Bieńkiewicz M, et al. (2010). "Single-nucleotide polymorphisms and mRNA expression for melatonin synthesis rate-limiting enzyme in recurrent depressive disorder". J. Pineal Res. 48 (4): 311–7. doi:10.1111/j.1600-079X.2010.00754.x. PMID 20433639.
- Yi H, Donohue SJ, Klein DC, McBride OW (1993). "Localization of the hydroxyindole-O-methyltransferase gene to the pseudoautosomal region: implications for mapping of psychiatric disorders". Hum. Mol. Genet. 2 (2): 127–31. doi:10.1093/hmg/2.2.127. PMID 8098975.
- Sato T, Deguchi T, Ichikawa T, et al. (1991). "Localization of hydroxyindole O-methyltransferase-synthesizing cells in bovine epithalamus: immunocytochemistry and in-situ hybridization". Cell Tissue Res. 263 (3): 413–8. doi:10.1007/BF00327275. PMID 1878930.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Rodriguez IR, Mazuruk K, Schoen TJ, Chader GJ (1994). "Structural analysis of the human hydroxyindole-O-methyltransferase gene. Presence of two distinct promoters". J. Biol. Chem. 269 (50): 31969–77. PMID 7989373.
- Stefulj J, Hörtner M, Ghosh M, et al. (2001). "Gene expression of the key enzymes of melatonin synthesis in extrapineal tissues of the rat". J. Pineal Res. 30 (4): 243–7. doi:10.1034/j.1600-079X.2001.300408.x. PMID 11339514.
- Melke J, Goubran Botros H, Chaste P, et al. (2008). "Abnormal melatonin synthesis in autism spectrum disorders". Mol. Psychiatry. 13 (1): 90–8. doi:10.1038/sj.mp.4002016. PMC 2199264. PMID 17505466.