Magnus Ma
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editHello, Magnus Ma, and welcome to Wikipedia! My name is Ian and I work with the Wiki Education Foundation; I help support students who are editing as part of a class assignment.
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If you have any questions, please don't hesitate to contact me on my talk page. Ian (Wiki Ed) (talk) 18:27, 13 October 2016 (UTC)
Peer Review 10/20 G1 Erin R
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The overall content of the introductory portion was satisfactory. As someone with no background knowledge of this subject, I could comprehend what was being said, but extra information about FRET technology in general and the difference between 'regular' FRET and smFRET may have been helpful. Also, I would recommend defining terms you will be using throughout the article early on. For example, ‘FRET efficiencies’ and ‘channel motifs’ could be expanded upon.
Each of the subheadings was of satisfactory length, although the unedited Methodology portion was a bit lengthy. It may be possible to have two subcategories of methodology, one for surface immobilized molecules and one for freely-diffusing molecules.
As you continue to edit this page, I would add more links to other Wikipedia articles, such as 'RNA folding,' 'native state,' 'dynamic temporal resolution,' and 'accumulation' in Advantages of FRET and 'KirBac potassium channels,' 'Force probe techniques,' and 'fluorophores' in Applications.
The example of channel motifs presented in the Applications was useful in visualizing the purpose of smFRET, but I recommend reorganizing to present the goal of the study first and then later highlighting how smFRET technology is useful in this scenario. The content of the example is good, I liked that there was a good explanation as to why smFRET was used over the averaged crystal structure method. I thought that the limitations section could use an example as well as this was a very broad overview.
The figure illustrated in this article helps visualize the importance of using a method, which is more specific than 'averaging,' however, the image could be polished to look a little more professional. Also, if it is possible, it could be beneficial to add example conformations of molecules to assign to each peak to understand what 'averaging' looks like in a molecular sense. Also, since this is an article about an imaging method, adding one of those images would help the reader comprehend what they are learning about (or a FRET population table). Another helpful figure could be an equation used to mathematically visualize 'distances between molecules as a function against time.' References are complete and the ones chosen show an array of source types, like journal articles and a book.
Overall, I think the article does a good job illustrating the importance of this technology in science today. The sections Advantages of smFRET and Applications contain some repeated ideas, like the imaging of averaged vs transition compositions of molecules. I would review these sections together and find a way to remove redundancy. The form could also be changed so that it reads less like a paper and more like a wiki article. Transition phrases can be removed. In Applications, make sure to change the tense into third person. Limitations seems a little vague and can be expanded and reworded so run-on sentences are not used. Erin R (Chem 455/505 Group 1) 20:38, 19 October 2016 (UTC) — Preceding unsigned comment added by Goblue2017 (talk • contribs)