About Me
editI am currently enrolled in Biology 3595A - Advanced Genetics at the University of Western Ontario.
I'm a third year student pursuing a Double Major in Computer Science and Genetics.
Draft
editA ribosomal binding site (RBS) is a sequence of nucleotides upstream of the start codon of an mRNA transcript that is responsible for the recruitment of a ribosome during the initiation of protein translation.
History
editThe Shine-Dalgarno sequence, of the prokaryotic RBS, was discovered by John Shine and Lynne Dalgarno in 1975.[1][2]
The Kozak consensus sequence was first identified by Marilyn Kozak in 1984[3] while she was in the Department of Biological Sciences at the University of Pittsburgh.[4]
Eukaryotes
edit5' cap
editRibosome recruitment in eukaryotes happens when eukaryote initiation factors elF4F and poly(A) binding protein (PABP) recognize the 5' capped mRNA and recruit the 43S ribosome complex at that location.[5]
Initiation happens following recruitment of the ribosome, at the start codon (underlined) found within the Kozak consensus sequence ACCAUGG. Since the Kozak sequence itself is not involved in the recruitment of the ribosome, it is not considered a ribosomal binding site.[5][6]
Internal ribosome entry site (IRES)
editEukaryotic ribosomes are known to bind to transcripts in a mechanism unlike the one involving the 5' cap, at a sequence called the internal ribosome entry site. This process is factor-independent and is commonly found in the translation of viral mRNA.[7]
Prokaryotes
editThe RBS in prokaryotes is a region upstream of the start codon This region is called the Shine-Dalgarno (SD) sequence.[2] The SD sequence on a messenger RNA is rich in purines and has the consensus AGGAGG. The complementary sequence (CCCUCCU), called the anti-Shine-Dargarno (ASD) is contained in the 3’ end of the 16S region of the smaller (30S) ribosomal subunit. Upon encountering the Shine-Dalgarno sequence, the ASD of the ribosome base-pairs with it, after which translation is initiated.[8][6]
Effect on translation initiation rate
editRibosomes begin translation of the mRNA transcript while DNA is still being transcribed. Thus translation and transcription are parallel processes. Bacterial mRNA are usually polycistronic and contain multiple ribosomal binding sites. Translation initiation is the most highly regulated step of protein synthesis.[9]
The rate of translation depends on two factors:
- the rate at which a ribosome is recruited to the RBS
- the rate at which a recruited ribosome is able to initiate translation (i.e. the translation initiation efficiency)
The RBS sequence affects both of these factors.
Factors affecting rate of ribosome recruitment
editThe ribosomal protein S1 binds to adenine sequences upstream of the RBS. Increasing the concentration of adenine upstream of the RBS will increase the rate of ribosome recruitment.[9]
Factors affecting the efficiency of translation initiation
editThe level of complementarity of the mRNA SD sequence to the ribosomal ASD greatly affects the efficiency of translation initiation. Richer complementarity results in higher initiation efficiency.[10] It is worth noting that this only holds up to a certain point - having too rich of a complementarity is known to paradoxically decrease the rate of translation as the ribosome then happens to be bound too tightly to proceed downstream.[10]
The optimal distance between the RBS and the start codon is variable - it depends on the the portion of the SD sequence encoded in the actual RBS and its distance to the start site of a consensus SD sequence. Optimal spacing increases the rate of translation initiation once a ribosome has been bound.[10] The composition of nucleotides in the spacer region itself was also found to affect the rate of translation initiation in one study[11].
Heat shock proteins
editSecondary structures formed by the RBS can affect the translational efficiency of mRNA, generally inhibiting translation. These secondary structures are formed by H-bonding of the mRNA base pairs and are sensitive to temperature. At a higher-than-usual temperature (~42 °C), the RBS secondary structure of heat shock proteins becomes undone thus allowing ribosomes to bind and initiate translation. This mechanism allows a cell to quickly respond to an increase in temperature.[9]
Gene annotation
editThe identification of RBS is used to determine the site of translation initiation an unannotated sequence. This is referred to as N-terminal prediction. This is especially useful when multiple start codons are situated around the potential start site of the protein.[12][13]
It is particularly difficult to identify RBS because they tend to be highly degenerated.[14] One approach to identifying RBS in E.coli is using neural networks.[15] Another approach is using the Gibbs sampling method.[12]
See also
editReferences
edit- ^ "Prof John Shine — Garvan Institute of Medical Research". www.garvan.org.au. Retrieved 2015-11-10.
- ^ a b Shine, J.; Dalgarno, L. (1975-03-06). "Determinant of cistron specificity in bacterial ribosomes". Nature. 254 (5495): 34–38. doi:10.1038/254034a0.
- ^ Kozak, Marilyn (1984-01-25). "Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs". Nucleic Acids Research. 12 (2): 857–872. doi:10.1093/nar/12.2.857. ISSN 0305-1048. PMID 6694911.
- ^ "Research: Top 10 Women Scientists Of The '80s: Making A Difference | The Scientist Magazine®". The Scientist. Retrieved 2015-11-10.
- ^ a b Hellen, Christopher U. T.; Sarnow, Peter (2001-07-01). "Internal ribosome entry sites in eukaryotic mRNA molecules". Genes & Development. 15 (13): 1593–1612. doi:10.1101/gad.891101. ISSN 0890-9369. PMID 11445534.
- ^ a b "Ribosomal Binding Site Sequence Requirements". www.thermofisher.com. Retrieved 2015-10-16.
- ^ Pisarev, Andrey V.; Shirokikh, Nikolay E.; Hellen, Christopher U.T. "Translation initiation by factor-independent binding of eukaryotic ribosomes to internal ribosomal entry sites". Comptes Rendus Biologies. 328 (7): 589–605. doi:10.1016/j.crvi.2005.02.004.
- ^ "Help:Ribosome Binding Site - parts.igem.org". parts.igem.org. Retrieved 2015-10-16.
- ^ a b c Laursen, Brian Søgaard; Sørensen, Hans Peter; Mortensen, Kim Kusk; Sperling-Petersen, Hans Uffe (2005-03-01). "Initiation of Protein Synthesis in Bacteria". Microbiology and Molecular Biology Reviews. 69 (1): 101–123. doi:10.1128/MMBR.69.1.101-123.2005. ISSN 1092-2172. PMC 1082788. PMID 15755955.
- ^ a b c De Boer, Herman A.; Hui, Anna S. (1990-01-01). Enzymology, BT - Methods in (ed.). [9] Sequences within ribosome binding site affecting messenger RNA translatability and method to direct ribosomes to single messenger RNA species. Gene Expression Technology. Vol. 185. Academic Press. pp. 103–114.
- ^ Stormo, Gary D.; Schneider, Thomas D.; Gold, Larry M. (1982-05-11). "Characterization of translational initiation sites in E. coli". Nucleic Acids Research. 10 (9): 2971–2996. doi:10.1093/nar/10.9.2971. ISSN 0305-1048. PMID 7048258.
- ^ a b Hayes, William S.; Borodovsky, Mark (1998). "Deriving ribosomal binding site (RBS) statistical models from unannotated DNA sequences and the use of the RBS model for N-terminal prediction" (PDF). Pacific Symposium on Biocomputing. 3: 279–290.
- ^ Noguchi, Hideki; Taniguchi, Takeaki; Itoh, Takehiko (2008-12-01). "MetaGeneAnnotator: Detecting Species-Specific Patterns of Ribosomal Binding Site for Precise Gene Prediction in Anonymous Prokaryotic and Phage Genomes". DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes. 15 (6): 387–396. doi:10.1093/dnares/dsn027. ISSN 1340-2838. PMC 2608843. PMID 18940874.
- ^ Oliveira, Márcio Ferreira da Silva; Mendes, Daniele Quintella; Ferrari, Luciana Itida; Vasconcelos, Ana Tereza Ribeiro. "Ribosome binding site recognition using neural networks". Genetics and Molecular Biology. 27 (4): 644–650. doi:10.1590/S1415-47572004000400028. ISSN 1415-4757.
- ^ Stormo, Gary D. (2000-01-01). "DNA binding sites: representation and discovery". Bioinformatics. 16 (1): 16–23. doi:10.1093/bioinformatics/16.1.16. ISSN 1367-4803. PMID 10812473.