Wikipedia

User:Jmudukes88/sandbox

< User:Jmudukes88

This is my sandbox.

Tom

Use the links associated w/ this to help build, edit a sandbox page: http://www.youtube.com/watch?v=pAy_kBBqs0U

Five Pillars Summary

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The Five Pillars of Wikipedia, here

  1. Wikipedia is an encyclopedia made by it's users
  2. Wikipedia does not hold any political, religious, or any type of bias
  3. Wikipedia is free to use!
  4. All interactions with Wiki users should be friendly
  5. There are no rules!

Summary Of Characteristics Of Target Article

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Good articles are:

1. Well-written:
a. respects copyright laws
b. correct spelling and grammer
2. Verifiable with no original research:
a. contains all references
b. provides the correct citations in-text
3. Broad Coverage:
a. covers material and does not stray from topic
4. Neutral:
a. It offers unbiased viewpoints
5. Stable:
a. NO editing wars
6. Well-illustrated with images:
a. Images are relevant
b. Images are referenced correctly

Using Citation Practice

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Telomeres are found at the ends two ends of linear chromosomes. The ends of chromosomes are packed with telomeric proteins that distinguish the ends of the chromosome in order to protect the DNA molecule from degradation or DNA repair activities[1] . The length of telomeres have been discussed as a potential biomarker of aging and general health status. Studies have associated shorter telomeres with cancer and other age-related diseases[1] . For example, idiopathic pulmonary fibrosis (IPF) is the most common disease related to telomere-shortening[2].

Notes

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  1. ^ a b Vera, Elsa; Blasco, Maria A. (2012 Jun). "Beyond average: potential for measurement of short telomeres". Aging. 4 (6): 379–92. doi:10.18632/aging.100462. PMC 3409675. PMID 22683684. {{cite journal}}: Check date values in: |date= (help)
  2. ^ Armanios M (February 2012). "Telomerase and idiopathic pulmonary fibrosis". Mutat. Res. 730 (1–2): 52–8. doi:10.1016/j.mrfmmm.2011.10.013. PMC 3292861. PMID 22079513.{{cite journal}}: CS1 maint: date and year (link)

Genomic Library/Preliminary Outline Of Article

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  • Our textbook summarizes what a genomic library is very well
  • A DNA library is a population of identical vectors each containing a different insert of DNA
  • Transformed cells take up the DNA
  • Each colony on media contains the same DNA from it's respective insert[1]
  • The book Genetics: From Genes to Genomes also gives a good overview of genomic libraries, but in a little more detail than our textbook. [2]
  • Link to Library (biology)?

History

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  • First DNA based genome to be sequenced by Frederick Sanger in 1977
  • Sequenced by making genomic library[3]
  • Link to Phi X 174?

Genomic Library Construction

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  http://commons.wikimedia.org/wiki/File:Genomic_Library.png

 
Plasmid replication (english)

http://commons.wikimedia.org/wiki/File:Plasmid_replication_(english).svg

Types Of Vectors Used

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Link to vector?

  • Genetics: From Genes to Genomes has a chart of different kinds of vectors used for genomic libraries and what size inserts each can hold. [2]

Image retrieved from wikipedia commons. http://commons.wikimedia.org/wiki/File:Genomic_Library.png

  • Molecular Cloning describes each kind of vector used for genomic libraries in detail (Cosmid, P1, PAC, BAC, and YAC). [5]

How To Select Vector??

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  • Displays mathematical model of how to select vector (how many clones needed to represent library and at what confidence level)
  • Details specific vectors, size, advantages/disadvantages, and examples
  • Provides key principles of vector selection
  • Great, detailed overview of Genomic library creation
  • Is this referenced appropriately? [6]
  • Genetics: From Genes to Genomes also explains some pros and cons to vectors and factors affecting the decision of which vector to use. It mentions too how to determine how many clones will be needed for coverage of a whole genome, but does not go into detail about calculations for coverage/confidence level. [2]

Applications

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Provide examples of these:

  • genetic engineering
  • genetic mapping and analysis in different organisms
  • analysis of healthy and diseased, etc.
  • The book A Primer of Genome Science covers the use of genomic libraries for hierarchical sequencing. [7]
  • BAC libraries had a role in sequencing the human genome. [8]
  • BAC libraries can be made with small fragments from bacterial genomic DNA in order to study gene function of bacteria that can't be cultured easily (pathogens) [9]

Current Use

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Clinical Use??

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References

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  1. ^ a b Losick, Richard; Watson, James D.; Tania A. Baker; Bell, Stephen; Gann, Alexander; Levine, Michael W. (2008). Molecular biology of the gene. San Francisco: Pearson/Benjamin Cummings. ISBN 978-0-8053-9592-1.{{cite book}}: CS1 maint: multiple names: authors list (link)
  2. ^ a b c Hartwell, Leland (2008). Genetics: from genes to genomes. Boston: McGraw-Hill Higher Education. ISBN 978-0-07-284846-5.
  3. ^ Sanger F, Air GM, Barrell BG; et al. (February 1977). "Nucleotide sequence of bacteriophage phi X174 DNA". Nature. 265 (5596): 687–95. doi:10.1038/265687a0. PMID 870828. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link)
  4. ^ Choi S, Kim UJ (2001). "Construction of a bacterial artificial chromosome library". Genomics Protocols. Methods Mol. Biol. Vol. 175. pp. 57–68. doi:10.1385/1-59259-235-X:057. ISBN 1-59259-235-X. PMID 11462856.
  5. ^ Russell, David W.; Sambrook, Joseph (2001). Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory. ISBN 0-87969-577-3.{{cite book}}: CS1 maint: multiple names: authors list (link)
  6. ^ [www.ucl.ac.uk/~ucbhjow/b200/Cloning_genomic_DNA.doc "Cloning Genomic DNA"]. University College London. Retrieved 13 March 2013. {{cite web}}: Check |url= value (help)
  7. ^ Muse, Spencer V.; Gibson, Greg (2004). A primer of genome science. Sunderland, Mass: Sinauer Associates. ISBN 0-87893-232-1.{{cite book}}: CS1 maint: multiple names: authors list (link)
  8. ^ Pareek CS, Smoczynski R, Tretyn A (November 2011). "Sequencing technologies and genome sequencing". J. Appl. Genet. 52 (4): 413–35. doi:10.1007/s13353-011-0057-x. PMC 3189340. PMID 21698376.{{cite journal}}: CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link)
  9. ^ Smajs D, Norris SJ, Weinstock GM (2004). "Construction of small genome BAC library for functional and genomic applications". Bacterial Artificial Chromosomes. Methods Mol. Biol. Vol. 255. pp. 47–56. doi:10.1385/1-59259-752-1:047. ISBN 1-59259-752-1. PMID 15020814.{{cite book}}: CS1 maint: multiple names: authors list (link)
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