Rosalina Tuyuc Velásquez (born San Juan Comalapa, department of Chimaltenango, 1956 - 2014  is a Guatemalan human rights activist. She was elected as a Congressional deputy in 1995, elected from the national list of the New Guatemala Democratic Front, and served as Vice President of Congress during that period. Tuyuc is a Kaqchikel Mayan.
In June 1982, the Guatemalan Army kidnapped and murdered her father, Francisco Tuyuc. Three years later, on 24 May 1985, her husband suffered the same fate. In 1988, she founded the National Association of Guatemalan Widows (CONAVIGUA), which has become a leading Guatemalan human rights organization.
In 1994, Tuyuc was decorated by the French Ordre national de la Légion d'honneur for her humanitarian activities. On 6 July 2004 President Óscar Berger appointed her to chair the National Reparations Commission (Comisión Nacional de Resarcimiento). In 2011, she publicly criticized the Commission for its failure to adequately address the damage caused by the war.
The Niwano Peace Foundation of Japan awarded their 2012 Niwano Peace Prize to Tuyuc "in recognition of her extraordinary and dogged work for peace as a courageous human rights activist and leader." 
- Entrevue avec Rosalina Tuyuc Projet Accompagnement Québec-Guatemala, 2003-11-17
- Rosalina Tuyuc: verità per il Guatemala 2003-04-02
- "Mayan Pupils Allowed to Wear Traditional Attire". New York Times. 1999-04-08. Retrieved 2008-06-23.
- Rico, Maite (December 1999). "We can't forgive until we have justice". The UNESCO Courier. Archived from the original on November 27, 2004. Retrieved 2008-06-23.
- Martinez, Emma (January 1994). "A November Full of Pitfalls". Revista Envio (150)..
- School of the Americas Watch
- Country Reports on Human Rights Practices for 2004: Guatemala United States Department of State, 2005-02-28
- Valdez, Sandra (May 31, 2011). "Rosalina Tuyuc: "Vemos con mucha indignación al PNR"". Prensa Libre. Retrieved 2011-05-31..
- "Guatemalan Human Rights Activist Rosalina Tuyuc Receives 29th Niwano Peace Prize". United News Wire. May 10, 2012..
- Biographical information from PeaceWomen Across the Globe.