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Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene.[5]

SPI1
Protein SPI1 PDB 1pue.png
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesSPI1, OF, PU.1, SFPI1, SPI-1, SPI-A, Spi-1 proto-oncogene
External IDsOMIM: 165170 MGI: 98282 HomoloGene: 2346 GeneCards: SPI1
Gene location (Human)
Chromosome 11 (human)
Chr.Chromosome 11 (human)[1]
Chromosome 11 (human)
Genomic location for SPI1
Genomic location for SPI1
Band11p11.2Start47,354,860 bp[1]
End47,378,576 bp[1]
RNA expression pattern
PBB GE SPI1 205312 at fs.png
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001080547
NM_003120

NM_011355

RefSeq (protein)

NP_001074016
NP_003111

NP_035485

Location (UCSC)Chr 11: 47.35 – 47.38 MbChr 2: 91.08 – 91.12 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

FunctionEdit

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development.[6] The nuclear protein binds to a purine-rich sequence known as the PU-box found on enhancers of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.[7]

StructureEdit

The ETS domain is the DNA-binding module of PU.1 and other ETS-family transcription factors.

InteractionsEdit

SPI1 has been shown to interact with:

See alsoEdit

ReferencesEdit

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000066336 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000002111 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Ray D, Culine S, Tavitain A, Moreau-Gachelin F (July 1990). "The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors". Oncogene. 5 (5): 663–8. PMID 1693183.
  6. ^ Oikawa, T; Yamada, T; Kihara-Negishi, F; Yamamoto, H; Kondoh, N; Hitomi, Y; Hashimoto, Y (July 1999). "The role of Ets family transcription factor PU.1 in hematopoietic cell differentiation, proliferation and apoptosis". Cell Death Differ. 6 (7): 599–608. doi:10.1038/sj.cdd.4400534. PMID 10453070.
  7. ^ "Entrez Gene: SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1".
  8. ^ Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F (February 1998). "The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS". J. Biol. Chem. 273 (9): 4838–42. doi:10.1074/jbc.273.9.4838. PMID 9478924.
  9. ^ Zhang P, Behre G, Pan J, Iwama A, Wara-Aswapati N, Radomska HS, Auron PE, Tenen DG, Sun Z (July 1999). "Negative cross-talk between hematopoietic regulators: GATA proteins repress PU.1". Proc. Natl. Acad. Sci. U.S.A. 96 (15): 8705–10. doi:10.1073/pnas.96.15.8705. PMC 17580. PMID 10411939.
  10. ^ Brass AL, Zhu AQ, Singh H (February 1999). "Assembly requirements of PU.1-Pip (IRF-4) activator complexes: inhibiting function in vivo using fused dimers". EMBO J. 18 (4): 977–91. doi:10.1093/emboj/18.4.977. PMC 1171190. PMID 10022840.
  11. ^ Escalante CR, Shen L, Escalante MC, Brass AL, Edwards TA, Singh H, Aggarwal AK (July 2002). "Crystallization and characterization of PU.1/IRF-4/DNA ternary complex". J. Struct. Biol. 139 (1): 55–9. doi:10.1016/s1047-8477(02)00514-2. PMID 12372320.
  12. ^ Hallier M, Tavitian A, Moreau-Gachelin F (May 1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. 271 (19): 11177–81. doi:10.1074/jbc.271.19.11177. PMID 8626664.

Further readingEdit

External linksEdit