MED14

MED14
Identifiers
Aliases	MED14, CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, RGR1, TRAP170, mediator complex subunit 14
External IDs	OMIM: 300182; MGI: 1349442; HomoloGene: 22082; GeneCards: MED14; OMA:MED14 - orthologs
Gene location (Human)
Chr.	X chromosome (human)
End	40,735,858 bp
Gene location (Mouse)
Chr.	X chromosome (mouse)
End	12,628,312 bp
RNA expression pattern
	Top expressed in
	secondary oocyte; ; jejunal mucosa; ; cartilage tissue; ; muscle of thigh; ; gastrocnemius muscle; ; ventricular zone; ; vastus lateralis muscle; ; biceps brachii; ; skeletal muscle tissue; ; Skeletal muscle tissue of rectus abdominis;
	Top expressed in
	genital tubercle; ; tail of embryo; ; medullary collecting duct; ; hair follicle; ; condyle; ; renal corpuscle; ; secondary oocyte; ; endocardial cushion; ; atrioventricular valve; ; conjunctival fornix;
	More reference expression data
	; ; ; ;
	More reference expression data
Gene ontology
Molecular function	transcription coactivator activity; transcription coregulator activity; protein binding; nuclear receptor coactivator activity; vitamin D receptor binding; signaling receptor activity;
Cellular component	membrane; core mediator complex; mediator complex; nucleoplasm; nucleus;
Biological process	androgen receptor signaling pathway; regulation of transcription, DNA-templated; regulation of transcription by RNA polymerase II; intracellular steroid hormone receptor signaling pathway; transcription, DNA-templated; stem cell population maintenance; positive regulation of transcription, DNA-templated; transcription initiation from RNA polymerase II promoter; positive regulation of transcription by RNA polymerase II;
	Sources:Amigo / QuickGO
Orthologs
	9282
	26896
	ENSG00000180182
	ENSMUSG00000064127
	O60244
	A2ABV5
	NM_004229
	NM_001048208; NM_012005
	NP_004220
	NP_001041673; NP_036135
	Wikidata
View/Edit Human	View/Edit Mouse

Mediator of RNA polymerase II transcription subunit 14 is an enzyme that in humans is encoded by the MED14 gene.^[5]^[6]^[7]

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.^[7]

Interactions

MED14 has been shown to interact with PPARGC1A,^[8] Estrogen receptor alpha,^[9] STAT2,^[10] Cyclin-dependent kinase 8,^[11]^[12] Glucocorticoid receptor^[13] and Hepatocyte nuclear factor 4 alpha.^[14]^[15]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000180182 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000064127 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1". Nature. 397 (6718): 446–50. Bibcode:1999Natur.397..446R. doi:10.1038/17141. hdl:11858/00-001M-0000-0019-A36A-8. PMID 9989412. S2CID 4405569.
^ Yoshikawa H, Fujiyama A, Nakai K, Inazawa J, Matsubara K (Aug 1998). "Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method". Genomics. 49 (2): 237–46. doi:10.1006/geno.1998.5246. PMID 9598311.
^ ^a ^b "Entrez Gene: CRSP2 cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa".
^ Wallberg, Annika E; Yamamura Soichiro; Malik Sohail; Spiegelman Bruce M; Roeder Robert G (Nov 2003). "Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1alpha". Mol. Cell. 12 (5): 1137–49. doi:10.1016/S1097-2765(03)00391-5. ISSN 1097-2765. PMID 14636573.
^ Kang, Yun Kyoung; Guermah Mohamed; Yuan Chao-Xing; Roeder Robert G (Mar 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2642–7. Bibcode:2002PNAS...99.2642K. doi:10.1073/pnas.261715899. ISSN 0027-8424. PMC 122401. PMID 11867769.
^ Lau, Joe F; Nusinzon Inna; Burakov Darya; Freedman Leonard P; Horvath Curt M (Jan 2003). "Role of metazoan mediator proteins in interferon-responsive transcription". Mol. Cell. Biol. 23 (2): 620–8. doi:10.1128/MCB.23.2.620-628.2003. ISSN 0270-7306. PMC 151539. PMID 12509459.
^ Wang, G; Cantin G T; Stevens J L; Berk A J (Jul 2001). "Characterization of mediator complexes from HeLa cell nuclear extract". Mol. Cell. Biol. 21 (14): 4604–13. doi:10.1128/MCB.21.14.4604-4613.2001. ISSN 0270-7306. PMC 87123. PMID 11416138.
^ Ito, M; Yuan C X; Malik S; Gu W; Fondell J D; Yamamura S; Fu Z Y; Zhang X; Qin J; Roeder R G (Mar 1999). "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators". Mol. Cell. 3 (3): 361–70. doi:10.1016/S1097-2765(00)80463-3. ISSN 1097-2765. PMID 10198638.
^ Hittelman, A B; Burakov D; Iñiguez-Lluhí J A; Freedman L P; Garabedian M J (Oct 1999). "Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins". EMBO J. 18 (19): 5380–8. doi:10.1093/emboj/18.19.5380. ISSN 0261-4189. PMC 1171607. PMID 10508170.
^ Maeda, Yutaka; Rachez Christophe; Hawel Leo; Byus Craig V; Freedman Leonard P; Sladek Frances M (Jul 2002). "Polyamines modulate the interaction between nuclear receptors and vitamin D receptor-interacting protein 205". Mol. Endocrinol. 16 (7): 1502–10. doi:10.1210/mend.16.7.0883. ISSN 0888-8809. PMID 12089346.
^ Malik, Sohail; Wallberg Annika E; Kang Yun Kyoung; Roeder Robert G (Aug 2002). "TRAP/SMCC/mediator-dependent transcriptional activation from DNA and chromatin templates by orphan nuclear receptor hepatocyte nuclear factor 4". Mol. Cell. Biol. 22 (15): 5626–37. doi:10.1128/MCB.22.15.5626-5637.2002. ISSN 0270-7306. PMC 133960. PMID 12101254.

Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
Fondell JD, Ge H, Roeder RG (1996). "Ligand induction of a transcriptionally active thyroid hormone receptor coactivator complex". Proc. Natl. Acad. Sci. U.S.A. 93 (16): 8329–33. Bibcode:1996PNAS...93.8329F. doi:10.1073/pnas.93.16.8329. PMC 38670. PMID 8710870.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
Sun X, Zhang Y, Cho H, et al. (1998). "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription". Mol. Cell. 2 (2): 213–22. doi:10.1016/S1097-2765(00)80131-8. PMID 9734358.
Gu W, Malik S, Ito M, et al. (1999). "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation". Mol. Cell. 3 (1): 97–108. doi:10.1016/S1097-2765(00)80178-1. PMID 10024883.
Ito M, Yuan CX, Malik S, et al. (1999). "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators". Mol. Cell. 3 (3): 361–70. doi:10.1016/S1097-2765(00)80463-3. PMID 10198638.
Rachez C, Lemon BD, Suldan Z, et al. (1999). "Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex". Nature. 398 (6730): 824–8. Bibcode:1999Natur.398..824R. doi:10.1038/19783. PMID 10235266. S2CID 204992765.
Näär AM, Beaurang PA, Zhou S, et al. (1999). "Composite co-activator ARC mediates chromatin-directed transcriptional activation". Nature. 398 (6730): 828–32. Bibcode:1999Natur.398..828N. doi:10.1038/19789. PMID 10235267. S2CID 23646963.
Hittelman AB, Burakov D, Iñiguez-Lluhí JA, et al. (1999). "Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins". EMBO J. 18 (19): 5380–8. doi:10.1093/emboj/18.19.5380. PMC 1171607. PMID 10508170.
Ge K, Guermah M, Yuan CX, et al. (2002). "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis". Nature. 417 (6888): 563–7. Bibcode:2002Natur.417..563G. doi:10.1038/417563a. PMID 12037571. S2CID 4432077.
Malik S, Wallberg AE, Kang YK, Roeder RG (2002). "TRAP/SMCC/mediator-dependent transcriptional activation from DNA and chromatin templates by orphan nuclear receptor hepatocyte nuclear factor 4". Mol. Cell. Biol. 22 (15): 5626–37. doi:10.1128/MCB.22.15.5626-5637.2002. PMC 133960. PMID 12101254.
Wang Q, Sharma D, Ren Y, Fondell JD (2003). "A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression". J. Biol. Chem. 277 (45): 42852–8. doi:10.1074/jbc.M206061200. PMID 12218053.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Lau JF, Nusinzon I, Burakov D, et al. (2003). "Role of metazoan mediator proteins in interferon-responsive transcription". Mol. Cell. Biol. 23 (2): 620–8. doi:10.1128/MCB.23.2.620-628.2003. PMC 151539. PMID 12509459.
Demirci FY, Ramser J, White NJ, et al. (2004). "Refinement of the physical location and the genomic characterization of the CRSP2 (EXLM1) gene on Xp11.4". DNA Seq. 14 (2): 123–7. doi:10.1080/1042517021000056952. PMID 12825353. S2CID 7902215.
Sato S, Tomomori-Sato C, Banks CA, et al. (2004). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex". J. Biol. Chem. 278 (50): 49671–4. doi:10.1074/jbc.C300444200. PMID 14576168.
Wallberg AE, Yamamura S, Malik S, et al. (2004). "Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1alpha". Mol. Cell. 12 (5): 1137–49. doi:10.1016/S1097-2765(03)00391-5. PMID 14636573.
Tomomori-Sato C, Sato S, Parmely TJ, et al. (2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". J. Biol. Chem. 279 (7): 5846–51. doi:10.1074/jbc.M312523200. PMID 14638676.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000180182 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000064127 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9989412-5] Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1". Nature. 397 (6718): 446–50. Bibcode:1999Natur.397..446R. doi:10.1038/17141. hdl:11858/00-001M-0000-0019-A36A-8. PMID 9989412. S2CID 4405569.

[pmid9598311-6] Yoshikawa H, Fujiyama A, Nakai K, Inazawa J, Matsubara K (Aug 1998). "Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method". Genomics. 49 (2): 237–46. doi:10.1006/geno.1998.5246. PMID 9598311.

[entrez-7] "Entrez Gene: CRSP2 cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa".

[pmid14636573-8] Wallberg, Annika E; Yamamura Soichiro; Malik Sohail; Spiegelman Bruce M; Roeder Robert G (Nov 2003). "Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1alpha". Mol. Cell. 12 (5): 1137–49. doi:10.1016/S1097-2765(03)00391-5. ISSN 1097-2765. PMID 14636573.

[pmid11867769-9] Kang, Yun Kyoung; Guermah Mohamed; Yuan Chao-Xing; Roeder Robert G (Mar 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2642–7. Bibcode:2002PNAS...99.2642K. doi:10.1073/pnas.261715899. ISSN 0027-8424. PMC 122401. PMID 11867769.

[pmid12509459-10] Lau, Joe F; Nusinzon Inna; Burakov Darya; Freedman Leonard P; Horvath Curt M (Jan 2003). "Role of metazoan mediator proteins in interferon-responsive transcription". Mol. Cell. Biol. 23 (2): 620–8. doi:10.1128/MCB.23.2.620-628.2003. ISSN 0270-7306. PMC 151539. PMID 12509459.

[pmid11416138-11] Wang, G; Cantin G T; Stevens J L; Berk A J (Jul 2001). "Characterization of mediator complexes from HeLa cell nuclear extract". Mol. Cell. Biol. 21 (14): 4604–13. doi:10.1128/MCB.21.14.4604-4613.2001. ISSN 0270-7306. PMC 87123. PMID 11416138.

[pmid10198638-12] Ito, M; Yuan C X; Malik S; Gu W; Fondell J D; Yamamura S; Fu Z Y; Zhang X; Qin J; Roeder R G (Mar 1999). "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators". Mol. Cell. 3 (3): 361–70. doi:10.1016/S1097-2765(00)80463-3. ISSN 1097-2765. PMID 10198638.

[pmid10508170-13] Hittelman, A B; Burakov D; Iñiguez-Lluhí J A; Freedman L P; Garabedian M J (Oct 1999). "Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins". EMBO J. 18 (19): 5380–8. doi:10.1093/emboj/18.19.5380. ISSN 0261-4189. PMC 1171607. PMID 10508170.

[pmid12089346-14] Maeda, Yutaka; Rachez Christophe; Hawel Leo; Byus Craig V; Freedman Leonard P; Sladek Frances M (Jul 2002). "Polyamines modulate the interaction between nuclear receptors and vitamin D receptor-interacting protein 205". Mol. Endocrinol. 16 (7): 1502–10. doi:10.1210/mend.16.7.0883. ISSN 0888-8809. PMID 12089346.

[pmid12101254-15] Malik, Sohail; Wallberg Annika E; Kang Yun Kyoung; Roeder Robert G (Aug 2002). "TRAP/SMCC/mediator-dependent transcriptional activation from DNA and chromatin templates by orphan nuclear receptor hepatocyte nuclear factor 4". Mol. Cell. Biol. 22 (15): 5626–37. doi:10.1128/MCB.22.15.5626-5637.2002. ISSN 0270-7306. PMC 133960. PMID 12101254.

[5]

[6]

[7]

[1]

[2]

[3]

[4]

[8]

[9]

[10]

[11]

[12]

[13]

[14]

[15]

MED14

Interactions

References

Further reading