DDX1

ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene.

DDX1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4XW3
Identifiers
Aliases	DDX1, DBP-RB, UKVH5d, DEAD/H-box helicase 1, DEAD-box helicase 1
External IDs	OMIM: 601257; MGI: 2144727; HomoloGene: 3627; GeneCards: DDX1; OMA:DDX1 - orthologs
Gene location (Human) Chr. Chromosome 2 (human) Band 2p24.3 Start 15,591,178 bp End 15,634,346 bp
Gene location (Mouse) Chr. Chromosome 12 (mouse) Band 12|12 A1.1 Start 13,266,974 bp End 13,299,214 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Skeletal muscle tissue of biceps brachii right ventricle deltoid muscle triceps brachii muscle middle temporal gyrus tendon of biceps brachii parotid gland glutes Skeletal muscle tissue of rectus abdominis islet of Langerhans Top expressed in primitive streak spermatid spermatocyte ventricular zone epiblast maxillary prominence mandibular prominence dentate gyrus of hippocampal formation granule cell triceps brachii muscle temporal muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function RNA helicase activity DNA binding nucleotide binding helicase activity poly(A) binding chromatin binding transcription coregulator activity DNA/RNA helicase activity protein binding nucleic acid binding nuclease activity double-stranded RNA binding exonuclease activity hydrolase activity ATP binding RNA binding Cellular component cytoplasm membrane nucleoplasm tRNA-splicing ligase complex cytoplasmic stress granule cleavage body nucleus cytosol nucleolus mitochondrion ribonucleoprotein complex Biological process regulation of transcription, DNA-templated response to exogenous dsRNA mRNA processing response to virus protein localization to cytoplasmic stress granule transcription, DNA-templated multicellular organism development spliceosomal complex assembly DNA duplex unwinding double-strand break repair RNA secondary structure unwinding regulation of translational initiation tRNA splicing, via endonucleolytic cleavage and ligation tRNA processing nucleic acid phosphodiester bond hydrolysis immune system process positive regulation of myeloid dendritic cell cytokine production positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response defense response to virus regulation of nucleic acid-templated transcription Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1653 104721 Ensembl ENSG00000079785 ENSMUSG00000037149 UniProt Q92499 Q91VR5 RefSeq (mRNA) NM_004939 NM_134040 RefSeq (protein) NP_004930 NP_598801 Location (UCSC) Chr 2: 15.59 – 15.63 Mb Chr 12: 13.27 – 13.3 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.

Interactions

DDX1 has been shown to interact with HNRPK. DDX1 has also been tentatively shown to interact with hCLE/C14orf166/RTRAF and HSPC117 in a cap-binding complex that activates mRNA translation.

Further reading

• Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY (2006). "Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions". Cell Research. 15 (11–12): 923–34. doi:10.1038/sj.cr.7290370. PMID 16354571.
• Godbout R, Squire J (August 1993). "Amplification of a DEAD box protein gene in retinoblastoma cell lines". Proceedings of the National Academy of Sciences of the United States of America. 90 (16): 7578–82. Bibcode:1993PNAS...90.7578G. doi:10.1073/pnas.90.16.7578. PMC 47185. PMID 7689221.
• Kitajima Y, Yatsuki H, Zhang R, Matsuhashi S, Hori K (March 1994). "A novel human homologue of a dead-box RNA helicase family". Biochemical and Biophysical Research Communications. 199 (2): 748–54. doi:10.1006/bbrc.1994.1292. PMID 8135819.
• Akiyama K, Akao Y, Yokoyama M, Nakagawa Y, Noguchi T, Yagi K, Nishi Y (April 1999). "Expression of two dead box genes (DDX1 and DDX6) is independent of that of MYCN in human neuroblastoma cell lines". Biochemistry and Molecular Biology International. 47 (4): 563–8. doi:10.1080/15216549900201603. PMID 10319407. S2CID 45609314.
• Golembowski S, Gellrich S, von Zimmermann M, Rutz S, Lippert S, Audring H, Lorenz P, Sterry W, Jahn S (April 2000). "Clonal evolution in a primary cutaneous follicle center B cell lymphoma revealed by single cell analysis in sequential biopsies". Immunobiology. 201 (5): 631–44. doi:10.1016/s0171-2985(00)80080-7. PMID 10834319.
• Bléoo S, Sun X, Hendzel MJ, Rowe JM, Packer M, Godbout R (October 2001). "Association of human DEAD box protein DDX1 with a cleavage stimulation factor involved in 3'-end processing of pre-MRNA". Molecular Biology of the Cell. 12 (10): 3046–59. doi:10.1091/mbc.12.10.3046. PMC 60154. PMID 11598190.
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (January 2002). "Directed proteomic analysis of the human nucleolus". Current Biology. 12 (1): 1–11. Bibcode:2002CBio...12....1A. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033.
• Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ (October 2002). "An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K" (PDF). The Journal of Biological Chemistry. 277 (43): 40403–9. doi:10.1074/jbc.M206981200. PMID 12183465. S2CID 25252628.
• Fang J, Kubota S, Yang B, Zhou N, Zhang H, Godbout R, Pomerantz RJ (December 2004). "A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of Rev". Virology. 330 (2): 471–80. doi:10.1016/j.virol.2004.09.039. PMID 15567440.
• Krishnan V, Zeichner SL (December 2004). "Alterations in the expression of DEAD-box and other RNA binding proteins during HIV-1 replication". Retrovirology. 1: 42. doi:10.1186/1742-4690-1-42. PMC 543576. PMID 15588285.
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (January 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
• Fang J, Acheampong E, Dave R, Wang F, Mukhtar M, Pomerantz RJ (June 2005). "The RNA helicase DDX1 is involved in restricted HIV-1 Rev function in human astrocytes". Virology. 336 (2): 299–307. doi:10.1016/j.virol.2005.03.017. PMID 15892970.
• Kaneko S, Ohira M, Nakamura Y, Isogai E, Nakagawara A, Kaneko M (March 2007). "Relationship of DDX1 and NAG gene amplification/overexpression to the prognosis of patients with MYCN-amplified neuroblastoma". Journal of Cancer Research and Clinical Oncology. 133 (3): 185–92. doi:10.1007/s00432-006-0156-y. PMID 17028906. S2CID 9295969.
• Tu LC, Yan X, Hood L, Lin B (April 2007). "Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells". Molecular & Cellular Proteomics. 6 (4): 575–88. doi:10.1074/mcp.M600249-MCP200. PMID 17220478. S2CID 7203032.