Cleavage and polyadenylation specificity factor subunit 4 is a protein that in humans is encoded by the CPSF4 gene.[5][6][7]

CPSF4
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesCPSF4, CPSF30, NAR, NEB1, NEB-1, cleavage and polyadenylation specific factor 4
External IDsOMIM: 603052; MGI: 1861602; HomoloGene: 38216; GeneCards: CPSF4; OMA:CPSF4 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001081559
NM_006693
NM_001318160
NM_001318161
NM_001318162

NM_001291248
NM_001291249
NM_178576
NM_001374716

RefSeq (protein)

NP_001075028
NP_001305089
NP_001305090
NP_001305091
NP_006684

NP_001278177
NP_001278178
NP_848671
NP_001361645

Location (UCSC)Chr 7: 99.44 – 99.46 MbChr 5: 145.1 – 145.12 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs.

In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs.

Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.[7]

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000160917Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000029625Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Nemeroff ME, Barabino SM, Li Y, Keller W, Krug RM (Jul 1998). "Influenza virus NS1 protein interacts with the cellular 30 kDa subunit of CPSF and inhibits 3'end formation of cellular pre-mRNAs". Mol Cell. 1 (7): 991–1000. doi:10.1016/S1097-2765(00)80099-4. PMID 9651582.
  6. ^ Barabino SM, Hubner W, Jenny A, Minvielle-Sebastia L, Keller W (Aug 1997). "The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins". Genes Dev. 11 (13): 1703–16. doi:10.1101/gad.11.13.1703. PMID 9224719.
  7. ^ a b "Entrez Gene: CPSF4 cleavage and polyadenylation specific factor 4, 30kDa".

Further reading

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