Bile salt-dependent lipase

Bile salt-dependent lipase (or BSDL), also known as carboxyl ester lipase (or CEL) is an enzyme produced by the adult pancreas and aids in the digestion of fats. Bile salt-stimulated lipase (or BSSL) is an equivalent enzyme found within breast milk. BSDL has been found in the pancreatic secretions of all species in which it has been looked for. BSSL, originally discovered in the milk of humans and various other primates, has since been found in the milk of many animals including dogs, cats, rats, and rabbits.[5]

CEL
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesCEL, BAL, BSDL, BSSL, CELL, CEase, FAP, FAPP, LIPA, MODY8, Bile salt-dependent lipase, carboxyl ester lipase
External IDsOMIM: 114840; MGI: 88374; HomoloGene: 37529; GeneCards: CEL; OMA:CEL - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001807

NM_009885

RefSeq (protein)

NP_001798

NP_034015

Location (UCSC)Chr 9: 133.06 – 133.07 MbChr 2: 28.45 – 28.45 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Enzymatic activity

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More than 95% of the fat present in human milk and in infant formulas is in the form of triacylglycerols (TG).[6] In adults, TGs are thought to be broken down or hydrolyzed mainly by the colipase-dependent lipase (CDL) enzyme. In the newborn, CDL activity in the duodenum is lower than in adults.[6]

Both BSDL and BSSL have a broad substrate specificity and, like CDL, are capable of hydrolyzing triacylglycerides (in addition to phospholipids, esters of cholesterol, and lipid-soluble vitamins). In particular, they can hydrolyze esters of the essential fatty acids (n-3 and n-6 PUFAs) and DHA.[7] BSDL production in the newborn pancreas is quite low when compared with production in the mammary gland or adult pancreas.[8]

However, newborn infants absorb lipids relatively well, considering the low level of CDL and BSDL they produce. This observation has led to the suggestion that BSDL produced by lactating mammary gland and present within milk, may compensate for the low levels of other TG-digesting enzymes and aid newborns in lipid absorption. The importance of BSSL in breast milk for the preterm infant nutrition was suggested at 2007.[9] It was also directly shown recently.[10]

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000170835Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026818Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Swan JS, Hoffman MM, et al. (1992). "Two forms of human milk bile-salt-stimulated lipase". Biochem. J. 283 (1): 119–122. doi:10.1042/bj2830119. PMC 1131002. PMID 1567358.
  6. ^ a b Lombardo, D. (2001). "Bile salt-dependent lipase: its pathophysiological implications". Biochimica et Biophysica Acta. 1533 (1): 1–28. doi:10.1016/S1388-1981(01)00130-5. PMID 11514232.
  7. ^ *Murasugi A, Asami Y, Mera-Kikuchi Y (2001). "Production of recombinant human bile-salt-stimulated lipase in Pichia pastoris". Protein Expression and Purification. 23 (2): 282–288. doi:10.1006/prep.2001.1509. PMID 11676603.
  8. ^ Sbarra V, Bruneau N, et al. (1998). "Molecular cloning of the bile salt-dependent lipase of ferret lactating mammary gland: an overview of functional residues". Biochimica et Biophysica Acta. 1393 (1): 80–89. doi:10.1016/S0005-2760(98)00067-8. PMID 9714751.
  9. ^ Andersson Y, Sävman K, et al. (2007). "Pasteurization of mother's own milk reduces fat absorption and growth in preterm infants". Acta Paediatrica. 96 (10): 1445–1449. doi:10.1111/j.1651-2227.2007.00450.x. PMID 17714541. S2CID 995002.
  10. ^ Maggio, L.; Bellagamba, M.; et al. "A prospective, randomized, double-blind crossover study comparing rhBSSL (recombinant human Bile Salt Stimulated Lipase) and placebo added to infant formula during one week of treatment in preterm infants born before 32 weeks of gestational age: preliminary results" (PDF). Swedish Orphan Biovitrum.

Further reading

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