Alkb homolog 3, alpha-ketoglutaratedependent dioxygenase

AlkB homolog 3, alpha-ketoglutaratedependent dioxygenase is a protein that in humans is encoded by the ALKBH3 gene.

ALKBH3
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2IUW
Identifiers
Aliases	ALKBH3, ABH3, DEPC-1, DEPC1, PCA1, hABH3, alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase, alkB homolog 3, alpha-ketoglutaratedependent dioxygenase, alkB homolog 3, alpha-ketoglutarate dependent dioxygenase
External IDs	OMIM: 610603 MGI: 1916363 HomoloGene: 16393 GeneCards: ALKBH3
Gene location (Human) Chr. Chromosome 11 (human) Band 11p11.2 Start 43,880,811 bp End 43,920,274 bp
Gene location (Mouse) Chr. Chromosome 2 (mouse) Band 2|2 E1 Start 93,810,895 bp End 93,841,152 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right uterine tube Achilles tendon gastrocnemius muscle stromal cell of endometrium islet of Langerhans body of pancreas tibial nerve skin of abdomen left uterine tube minor salivary glands Top expressed in interventricular septum seminiferous tubule gastrocnemius muscle soleus muscle triceps brachii muscle skeletal muscle tissue vastus lateralis muscle knee joint digastric muscle brown adipose tissue More reference expression data BioGPS n/a
Gene ontology Molecular function protein binding L-ascorbic acid binding dioxygenase activity metal ion binding DNA-N1-methyladenine dioxygenase activity oxidoreductase activity ferrous iron binding mRNA N1-methyladenosine dioxygenase activity 2-oxoglutarate-dependent dioxygenase activity cytosine C-5 DNA demethylase activity Cellular component nucleus mitochondrion nucleoplasm cytoplasm cytosol Biological process cell population proliferation cellular response to DNA damage stimulus DNA dealkylation involved in DNA repair oxidative single-stranded DNA demethylation oxidative single-stranded RNA demethylation DNA repair Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 221120 69113 Ensembl ENSG00000166199 ENSMUSG00000040174 UniProt Q96Q83 Q8K1E6 RefSeq (mRNA) NM_139178 NM_026944 RefSeq (protein) NP_631917 NP_081220 Location (UCSC) Chr 11: 43.88 – 43.92 Mb Chr 2: 93.81 – 93.84 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).

Further reading

• Sundheim O, Vågbø CB, Bjørås M, Sousa MM, Talstad V, Aas PA, Drabløs F, Krokan HE, Tainer JA, Slupphaug G (2006). "Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage". EMBO J. 25 (14): 3389–97. doi:10.1038/sj.emboj.7601219. PMC 1523172. PMID 16858410.
• Liu BQ, Wu YD, Li PH, Wei JX, Zhang T, Liu RL (2007). "Prostate cancer antigen-1 as a potential novel marker for prostate cancer". Asian J. Androl. 9 (6): 821–6. doi:10.1111/j.1745-7262.2007.00279.x. PMID 17968469.
• Monsen VT, Sundheim O, Aas PA, Westbye MP, Sousa MM, Slupphaug G, Krokan HE (2010). "Divergent ß-hairpins determine double-strand versus single-strand substrate recognition of human AlkB-homologues 2 and 3". Nucleic Acids Res. 38 (19): 6447–55. doi:10.1093/nar/gkq518. PMC 2965238. PMID 20525795.
• Chen B, Liu H, Sun X, Yang CG (2010). "Mechanistic insight into the recognition of single-stranded and double-stranded DNA substrates by ABH2 and ABH3". Mol Biosyst. 6 (11): 2143–9. doi:10.1039/c005148a. PMID 20714506.
• Malki K, Uher R, Paya-Cano J, Binder E, Rietschel M, Zobel A, Mors O, Hauser J, Henigsberg N, Jerman B, Souery D, Placentino A, Ng MY, Cohen-Woods S, Sluyter F, Farmer A, Aitchison KJ, Craig IW, Lewis CM, McGuffin P, Schalkwyk LC (2011). "Convergent animal and human evidence suggests a role of PPM1A gene in response to antidepressants". Biol. Psychiatry. 69 (4): 360–5. doi:10.1016/j.biopsych.2010.08.011. PMID 20970119. S2CID 41571515.
• Tasaki M, Shimada K, Kimura H, Tsujikawa K, Konishi N (2011). "ALKBH3, a human AlkB homologue, contributes to cell survival in human non-small-cell lung cancer". Br. J. Cancer. 104 (4): 700–6. doi:10.1038/sj.bjc.6606012. PMC 3049579. PMID 21285982.
• Dango S, Mosammaparast N, Sowa ME, Xiong LJ, Wu F, Park K, Rubin M, Gygi S, Harper JW, Shi Y (2011). "DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation". Mol. Cell. 44 (3): 373–84. doi:10.1016/j.molcel.2011.08.039. PMC 3258846. PMID 22055184.
• Koike K, Ueda Y, Hase H, Kitae K, Fusamae Y, Masai S, Inagaki T, Saigo Y, Hirasawa S, Nakajima K, Ohshio I, Makino Y, Konishi N, Yamamoto H, Tsujikawa K (2012). "anti-tumor effect of AlkB homolog 3 knockdown in hormone- independent prostate cancer cells". Curr Cancer Drug Targets. 12 (7): 847–56. doi:10.2174/156800912802429283. PMID 22515525.
• Yamato I, Sho M, Shimada K, Hotta K, Ueda Y, Yasuda S, Shigi N, Konishi N, Tsujikawa K, Nakajima Y (2012). "PCA-1/ALKBH3 contributes to pancreatic cancer by supporting apoptotic resistance and angiogenesis". Cancer Res. 72 (18): 4829–39. doi:10.1158/0008-5472.CAN-12-0328. PMID 22826605. S2CID 134935.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.