In enzymology, an acetyl-CoA hydrolase (EC 188.8.131.52) is an enzyme that catalyzes the chemical reaction
acetyl-CoA + H2O CoA + acetate + H+
Thus, the two substrates of this enzyme are acetyl-CoA and H2O, whereas its two products are CoA and acetate. It is present in many species including animals (see reference below).
This enzyme belongs to the family of hydrolases, specifically those acting on thioester bonds. The systematic name of this enzyme class is acetyl-CoA hydrolase. Other names in common use include acetyl-CoA deacylase, acetyl-CoA acylase, acetyl coenzyme A hydrolase, acetyl coenzyme A deacylase, acetyl coenzyme A acylase, and acetyl-CoA thiol esterase. This enzyme participates in pyruvate metabolism.