Wikipedia talk:United States Education Program/Courses/JHU MolBio Ogg 2013/Group 82G

Article selection discussion

Hi Ryan, As follow up to your question (via email) regarding any articles to claim. Out the four we did, I like frameshift mutation and ribosomal RNA. As another RecA. Thoughts?DougCovert (talk) 03:21, 26 February 2013 (UTC)

I'm completely fine with either of the 2 choices for the wikipedia article, both have their pros and cons. I kind of like the frameshift mutation because anything we do to improve the page (adding sections and whatnot) will look like a giant improvement, but the rRNA has some better info so it may be easier to research. Either way I'm fine with whatever you think will be best!165.123.243.167 (talk) 14:14, 1 March 2013 (UTC)

Okay...lets do Frameshift mutation (FM). So go ahead and "claim it" for us if you would. The instructions in Unit 6 look fairly straight forward. Let me know if you have problems and I try, if not we'll get our OA to help. Here are my reasons that we can build upon along with yours for our 'rationale': its intriguing.....a small/simple error with large/complex outcomes; anything we do is a big improvement; I like the link it has to diseases...can we find more; further detailed research on why and how it occurs can be includedDougCovert (talk) 22:17, 1 March 2013 (UTC)

I think adding to the disease section would be great. I know at least a portion of the diseases I deal with at work have a frameshift mutation component, so I'm sure we will be able to find a good amount of information.165.123.243.167 (talk) 16:37, 2 March 2013 (UTC)

I added a topic header for your discussion. Klortho (talk) 14:55, 3 March 2013 (UTC)

Thanks, KlorthoDougCovert (talk) 18:06, 3 March 2013 (UTC)

Rationale Discussion

Hi Ryan; Great....diseases will be very important section for this article. Also,I sent you a start on our 'rationale'. It is an attached Word doc in our JHU email. Doug DougCovert (talk) 18:06, 3 March 2013 (UTC)

Everything looked great. I threw in a few more comments as well on things that we can look to improve. I also found some links that I think will be helpful in the disease section http://www.sanger.ac.uk/genetics/CGP/Census/frameshift_mutation.shtml and http://www.medscape.com/viewarticle/584255_5 are just a few. I think this will definitely be an interesting topic to work with. Sharkeyr (talk) 19:31, 3 March 2013 (UTC)

Hi Doug, I just wanted to throw it out there so I don't forget about it next week, reading through chapter 13 it mentions guide RNAs (gRNAs) as a way that frameshift muations are introduced, just something we may want to keep in mind for further research.Sharkeyr (talk) 01:17, 5 March 2013 (UTC)

Good Thought Ryan, I inputted the heading for our summary. When ready place in our rationale. ThanksDougCovert (talk) 15:16, 5 March 2013 (UTC)

The rationale has been added. Here is another article that may be of use. http://vir.sgmjournals.org/content/85/8/2389.full.pdf Sharkeyr (talk) 19:48, 5 March 2013 (UTC)

Hi Ryan,I've been sick the last two days. You may have mis-understood my JHU email from yesterday morning. Sorry about that!Please DO add the links/refs as you suggested in your Monday's JHU email. Thanks.DougDougCovert (talk) 13:11, 6 March 2013 (UTC)

Hi Ryan, Looks Great.....Thanks for adding them! DougCovert (talk) 19:11, 6 March 2013 (UTC)

Unit 7 Discussion

Hi there Ryan, Hope you didn't get buried with any of the snow that went out that way towards DC. Anyways, how do you want to break up the work for unit 7? It looks like there are 4 major things for this unit. (ref list, images, prelim outline & improvement ideas) I've started a little organization (see attached in JHU emial that we can move to sandbox) for three of them. We could probably expand on our rationale for improvements. I don't know what you are leaning towards for researching info., so let me knowDougCovert (talk) 14:33, 8 March 2013 (UTC)

Hi Doug, as per our email I've started looking into some more references for the page. I'll also add some notes on the ones I sent last week. The disease category has an overwhelming amount of information, so I think to start we should focus on the causes and effects to lead into the topic of the role in disease, if that makes sense. I'll still note some points on the disease articles I found too.Sharkeyr (talk) 16:09, 9 March 2013 (UTC)

Hi Ryan, Sure, thats a good idea. Maybe we should breakup the disease section into subunits of specific disease types? Thoughts.DougCovert (talk) 19:29, 9 March 2013 (UTC)

Sure, what disease categories were you thinking? I know from what I've been looking at this week there are definitely articles talking about frameshift mutations in several cancers, cystic fibrosis and chron's disease, but I'm sure there are also plenty of others. Sharkeyr (talk) 23:03, 10 March 2013 (UTC)

I think what you stated above are perfect areasto divide up. Lets not forget tay sachs, and I found some about dogs and sheep....I'll send you. Okay so I've added to your sandbox, a start on an image list, and the preliminary outline. Also, below in this talk page a started a new section....for artcle improvements. I used the prelim outline as a baseline.DougCovert (talk) 01:16, 11 March 2013 (UTC)

Article Improvement Ideas

Group 82G selected frameshift mutation because this topic has limited information and few references. There are more key points, or concepts that will help develop this article from a Stub status. These are briefly listed below and will be content sections of the article with related images.

Molecular Biology and Biochemistry background

Provide background in molecular biology and biochemisty to understanding frameshift mutation will be included. - Central dogma - Brief background on translation (rRNA, tRNA processes) - Importance of the triplet codon in translation - Movement/deletion of the stop codon to alter entire protein - Protein: structure and function

Mechanism

More research data into what causes a frameshift mutation to occur are available for learning and understanding that can be effectively expanded upon in more detail. - Description of frameshift mutation - Is it heredity or environmental mutation - Debate why this is different than a point mutation

Frame shift causes-Research

The article did indicate a couple of areas of research into the exact process on how the shift (insertion or deletion) occurs, and thus are key points to upgrade. - Research attempts into what exactly cause the shift (deletion or insertion) - Methods for finding frameshift mutations - RNA editing (gRNAs)

Diseases-Research

There are projects for several disease types looking specifically at frameshift mutations that was not mentioned in the original article. - Diseases: research into causes and cures (cancers, cystic fibrosis, tay-sachs, etc.) - Keep concepts from “thermodynamic” section of original article to keep

Positive Uses-Research

Are there positive uses for frameshift mutation that would be beneficial industry or medicine. - Identification of frameshift mutations that can lead to improved treatments - Any positive uses for this type of mutation (nylonase?)

The remaining sections in the article will be geared towards providing more references. The original authors did provide direction for further reading and external links, and these will both be great starting points to enlarge this article’s reference base. Theses ideas for improvement are based upon the rationale and preliminary outline developed by Ryan and I.DougCovert (talk) 01:36, 11 March 2013 (UTC)

Article improvement discussion for frameshift mutation talk page

Hi Doug, I wrote up a quick blurb on what we are looking to improve to put on the talk page. Please feel free to edit wherever you'd like and just let me know when you'd like me to transfer it over.

To improve this article we are looking to broaden the basic ideas already highlighted. We would like to add in information regarding the formation of frameshift mutations and how they effect the reading frame/proteins. We would also like to look into the mechanism of the mutation, i.e. genetic or environmental causes, and also how it differs from other point mutations. From there we would like to expand into research on the cause of frame mutations. This will lead into the clinical relevancy of frame mutations, such as role in disease. Finally we would like to talk about frameshift mutations as a positive factor in dealing with improving treatments, and the role frameshift mutations play/have played in evolution, i.e. nylonase. Sharkeyr (talk) 21:30, 12 March 2013 (UTC)

Hi Ryan, This is a great addition. Go ahead and add it to the one started above. Should it go at the beginning...before or after the short three sentence intro?DougCovert (talk) 23:11, 12 March 2013 (UTC)