Wikipedia:United States Education Program/Courses/JHU MolBio Ogg 2013/Group 82B

Group 82B

This is the Wikipedia page for 410.602 Molecular Biology, Spring, 2013, group 82B. This group will be working on the article Ribonucleotide.

Use the talk page here to collaborate as a group, when learning to use and navigate Wikipedia, assessing articles, or for any other topic.

Use this page (not the talk page) for article assessments; rationale for selecting an article; etc.

Please create a new section here for each of those assignments.

Final Progress Report

Added more wiki links to the article
Shortened section headings
Created and uploaded table that shows the 4 major ribonucleotides
Added section for ribonucleotide synthesis and images to illustrate mechanism
Thanks to Bcheon1, Aluquette, Klortho, Jengel11, Jgould1400 for all your helpful comments, suggestions, and feedback. It really helped us revise our article.

Unit 9 Progress Report

Created table to show the different nucleotides. Most of our contributions are prose.

Initial article assessments from Jessicalau90

Oskar

This article provides no content and what is written is similar a dictionary definition. The article does not contain any references and thus, is not verifiable. It does not contain any supporting materials and there are no images to illustrate its point. Also, a talk page has not been created to allow other editors to discuss on ways to improve this article.

References

Oskar^[1]

Oskar 2^[2]

^ Ewen-Campen, B; Srouji, JR; Schwager, EE; Extavour, CG (4 December 2012). "Oskar predates the evolution of germ plasm in insects". Current Biology. 22 (23): 2278–83. doi:10.1016/j.cub.2012.10.019. PMID 23122849. S2CID 6682356.
^ Sanghavi, P; Lu, S; Gonsalvez, GB (1 July 2012). "A functional link between localized Oskar, dynamic microtubules, and endocytosis". Developmental Biology. 367 (1): 66–77. doi:10.1016/j.ydbio.2012.04.024. PMID 22561189.

Gene knockdown

This article gives a very basic description of the topic. It contains very minimal references and in line citations, hence, not all information is verifiable. Also, there are clean up banners present. There is no structure to the article and the information is not organized into groups of related material. The article does not contain any supporting material or images to illustrate its point. There is minimal activity on the talk page of the article.

References

Gene knockdown^[1]

Gene knockdown 2^[2]

^ Kleinhammer, A; Wurst, W; Kühn, R (2013). "Target Validation in Mice by Constitutive and Conditional RNAi". Target Identification and Validation in Drug Discovery. Methods in Molecular Biology. Vol. 986. pp. 307–23. doi:10.1007/978-1-62703-311-4_19. ISBN 978-1-62703-310-7. PMID 23436420.
^ Goldberg, MS (10 February 2013). "siRNA Delivery for the treatment of ovarian cancer". Methods. 63 (2): 95–100. doi:10.1016/j.ymeth.2013.01.007. PMID 23403216.

Initial article assessments from Binhtruong

Terminator (genetics)

Quick Evaluation:

1) Lacks content that is necessary to cover the topic adequately.

2) Lacks illustrations that support content for improved comprehension.

3) Lacks proper cited sources in addition to inline citations.

Improvements:

1) Provide more content that provides a broad and deeper understanding of the topic in hand.

2) Provide properly cited images or illustrations that improve understanding of the topic.

3) Provide abundant cited resources that support the topic.

Possible references that can be utilized:

1) Lodish, H., Berk, A., Zipursky, S.L., Matsudaira, P., Baltimore, D., Darnell, J., 2000, Molecular Cell Biology, 4th Ed., W.H. Freeman and Company, NY, New York. ISBN 0-7167-3136-3

2) Caballero VC, Toledo VP, Maturana C, Fisher CR, Payne SM, Salazar JC (2012). "Expression of Shigella flexneri gluQ-rs gene is linked to dksA and controlled by a transcriptional terminator". BMC Microbiol. 12: 226. doi:10.1186/1471-2180-12-226. PMC 3542578. PMID 23035718.

Nondisjunction

Quick Evaluation:

1) Lacks little content that is necessary to cover the topic adequately.

2) Provides some illustrations that support content for improved comprehension however description of illustration is quite vague.

3) Lacks proper cited sources in addition to inline citations.

Improvements:

1) Provide additional content that provides a broad and deeper understanding of the topic in hand.

2) Provide more properly cited images or illustrations in addition to descriptions that improve understanding of the topic.

3) Provide abundant cited resources that support the topic.

Possible references that can be utilized:

1) Gillies SC, Lane FM, Paik W, Pyrtel K, Wallace NT, Gilliland WD (February 2013). "Nondisjunctional segregations in Drosophila female meiosis I are preceded by homolog malorientation at metaphase arrest". Genetics. 193 (2): 443–51. doi:10.1534/genetics.112.146241. PMC 3567735. PMID 23222652.

2) Ghosh D, Gochhait S, Banerjee D, Chatterjee A, Sinha S, Nandagopal K (October 2012). "SNaPshot Assay in Quantitative Detection of Allelic Nondisjunction in Down Syndrome". Genet Test Mol Biomarkers. 16 (10): 1226–35. doi:10.1089/gtmb.2012.0083. PMC 3468158. PMID 22931243.

Article Selection Rationale

We selected ribonucleotide as our topic because we found that the current condition of the article requires major improvement. We were surprised to see that the content of the article was very limited for a topic that has been covered with depth in every fundamental molecular biology textbook. Currently, the article only gives a broad definition of a ribonucleotide. In addition, there are no diagrams or illustrations present that supports the content in the current article. Thus we are in agreement with Wikiproject that this topic is of high importance and will take on the responsibility of improving the article from stub to a class B/GA to the best of our ability. We can improve on the article by first presenting a general scope and description of ribonucleotides itself, followed by a more detailed description in later sections. A brief description behind the history and discovery of ribonucleotides maybe a nice way to set the tone, followed by a distinction between nucleotides of DNA and ribonucleotides of RNA. We would then explore the four units of ribonucleotide, followed by differentiation between purines and pyrimidines. Then perhaps elaborate on the biosynthesis of the purine and pyrimidine with a focus on the several enzymes involved. To build on the article, we could possibly elaborate on the roles that ribonucleotides play in RNA in addition to a detailed description of the essential steps ribonucleotides are involved in during transcription. We will also ensure that in-line citations from reliable sources are included.

[1] Ewen-Campen, B; Srouji, JR; Schwager, EE; Extavour, CG (4 December 2012). "Oskar predates the evolution of germ plasm in insects". Current Biology. 22 (23): 2278–83. doi:10.1016/j.cub.2012.10.019. PMID 23122849. S2CID 6682356.

[2] Sanghavi, P; Lu, S; Gonsalvez, GB (1 July 2012). "A functional link between localized Oskar, dynamic microtubules, and endocytosis". Developmental Biology. 367 (1): 66–77. doi:10.1016/j.ydbio.2012.04.024. PMID 22561189.

[3] Kleinhammer, A; Wurst, W; Kühn, R (2013). "Target Validation in Mice by Constitutive and Conditional RNAi". Target Identification and Validation in Drug Discovery. Methods in Molecular Biology. Vol. 986. pp. 307–23. doi:10.1007/978-1-62703-311-4_19. ISBN 978-1-62703-310-7. PMID 23436420.

[4] Goldberg, MS (10 February 2013). "siRNA Delivery for the treatment of ovarian cancer". Methods. 63 (2): 95–100. doi:10.1016/j.ymeth.2013.01.007. PMID 23403216.

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