User:Yanping Nora Soong/biochemnotes

GC Adv Biochem II BICM 71020

Week 1

• Metabolic control analysis

Papers

• Why did nature choose phosphates?
• Metabolic control analysis papers

Week 2

• Glycolysis v. Gluconeogenesis
• Cori cycle
• Fructose 2,6-bisphosphate and AMP as regulators of Phosphofructokinase

• PFK-1 / PFK-2

• Neuronal and hepatocyte isozymes of PFK-1

• Glucose transporters
  • GLUT1 (facilitated diffusion)
  • GLUT2 (liver/pancreas)
  • SGLT1 (symporter)

Week 3

• GPCRs
• beta-1 adrenergic receptor
  • activates Gs alpha subunit

Supplement

• Mechanisms of insulin resistance

Week 4

• Warburg effect
• Regulation of pyruvate dehydrogenase
  • Kinase / phosphatases
  • Product inhibition
• Allosteric regulators

Week 5

• Ketogenesis
• Cytosolic oxaloacetate is converted to malate. Two fates
  • Malate can be converted to pyruvate
  • Malate can enter the mitochondria and be converted into oxaloacetate

• Four major repeating steps of fatty acid synthesis
  • Condensation (ELOVL)
  • Reductase
  • Dehydratase
  • Reductase

Week 6

• Phenylketonuria
• Branched-chain amino acid catabolism (deficiency v. excess)
• Pyridoxal phosphate

Week 8

• Mobility of proteins in lipid bilayer
  • Fluorescence recovery after photobleaching (FRAP)
  • Fluorescence loss in photobleaching

Final

• Pentose phosphate pathway
• Purine biosynthesis
• Transporters

• GLUT1
• GLUT4 (insulin-dependent)