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There are some excellent videos related to cell biology. One of the best is The Inner Life of the Cell

mRNA

There are several isoforms of TMEM66. The two most commonly seen have all seven exons (XX nt) or lack exon 2 (YY nt). In addition to alternatively splicing, TMEM66 is uses alternative promoters. Comparative analysis of the total brain samples showed that TMEM66 has statistically significant usage of alternative promoters in AD samples ^[1].

Protein

A multiple sequence alignment can be a useful way to show important features of a human protein and its orthologs. Here is one for TMEM66^[2] .:

An excerpt of a multiple sequence alignment of TMEM66 proteins

Protein Interactions

TMEM66 has been found to interact with several proteins in two hybrid screen assays. Here is one network:

TMEM66 interaction network

Clinical Significance

A slight association with a the ability to quit smoking has been detected for one variant of TMEM66.

References

^ Twine, Natalie A.; Janitz, Karolina; Wilkins, Marc R.; Janitz, Michal (2011). "Whole Transcriptome Sequencing Reveals Gene Expression and Splicing Differences in Brain Regions Affected by Alzheimer's Disease". PLOS ONE. 6 (1): e16266. doi:10.1371/journal.pone.0016266. PMID 21283692.{{cite journal}}: CS1 maint: date and year (link)
^ Larkin, M.A. (2007). "Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., Lopez R., Thompson J.D., Gibson T.J. and Higgins D.G. (2007) ClustalW and ClustalX version 2". Bioinformatics. 23 (21): 2947–2948. doi:10.1093/bioinformatics/btm404. PMID 17846036. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)

[1] Twine, Natalie A.; Janitz, Karolina; Wilkins, Marc R.; Janitz, Michal (2011). "Whole Transcriptome Sequencing Reveals Gene Expression and Splicing Differences in Brain Regions Affected by Alzheimer's Disease". PLOS ONE. 6 (1): e16266. doi:10.1371/journal.pone.0016266. PMID 21283692.{{cite journal}}: CS1 maint: date and year (link)

[ClustalW-2] Larkin, M.A. (2007). "Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., Lopez R., Thompson J.D., Gibson T.J. and Higgins D.G. (2007) ClustalW and ClustalX version 2". Bioinformatics. 23 (21): 2947–2948. doi:10.1093/bioinformatics/btm404. PMID 17846036. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)

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