This is a list of railroads covered by American Shortline Railway Guide, 5th Edition (1996).
- A&G Railroad
- ← Abbeville-Grimes Railway (-1994)
- A&R Line
- Aberdeen and Rockfish Railroad
- Aberdeen, Carolina and Western Railway
- ← Aberdeen and Briar Patch Railway (-1987)
- Acadiana Railway
- Adrian and Blissfield Rail Road
- Akron Barberton Cluster Railway
- ← Akron and Barberton Belt Railroad (-1994)
- Alabama Railroad
- Alabama and Florida Railway
- ← Alabama and Florida Railroad (-1992)
- ← Andalusia and Conecuh Railroad (1983-1987, mostly abandoned)
- ← Alabama and Florida Railroad (-1992)
- Alameda Belt Line
- Alaska Railroad
- Albany Port Railroad
- Alexander Railroad
- Algers, Winslow and Western Railway
- Algoma Central Railway
- Aliquippa and Southern Railroad
- Allegheny and Eastern Railroad
- ← Allegheny Railroad (-1992)
- ← Johnsonburg, Kane, Warren and Irvine Railroad (1982-1985)
- ← Allegheny Railroad (-1992)
- Allegheny Valley Railroad
- Almanor Railroad
- Amador Central Railroad
- Angelina and Neches River Railroad
- Ann Arbor Railroad
- ← Michigan Interstate Railroad (-1988)
- Apache Railway
- Apalachicola Northern Railroad
- Appanoose County Community Railroad
- Arcade and Attica Railroad
- Arizona and California Railroad
- Arizona Central Railroad
- Arizona Eastern Railway
- Arkansas and Missouri Railroad
- Arkansas Central Railway
- Arkansas, Louisiana and Mississippi Railroad
- ← Arkansas and Louisiana Missouri Railway (-1991)
- Arkansas Midland Railroad
- Aroostook Valley Railroad
- Ashland Railway
- Ashtabula Carson Jefferson Railroad
- AT&L Railroad
- Atlanta, Stone Mountain and Lithonia Railway
- Atlantic and Gulf Railroad
- Atlantic and Western Railway
- Austin and Northwestern Railroad
- Bad Water Line
- Bangor and Aroostook Railroad
- Batten Kill Railroad
- Bauxite and Northern Railway
- Bay Colony Railroad
- Bay Line Railroad
- ← Atlanta and St. Andrews Bay Railway (-1994)
- BC Rail
- Beaufort and Morehead Railway
- Bee Line Railroad
- Beech Mountain Railroad
- Belfast and Moosehead Lake Railroad
- Belvidere and Delaware River Railway
- Berlin Mills Railway
- Bessemer and Lake Erie Railroad
- Bi-State Development Agency Railroad
- Birmingham Southern Railroad
- Black River and Western Corporation
- Bloomer Shippers Connecting Railroad
- Blue Mountain Railroad
- Blue Rapids Railway
- Blue Rock Transportation Company
- Border Pacific Railroad
- Bradford Industrial Rail, Inc.
- Brandon Corporation
- Brandywine Valley Railroad
- Bristol Industrial Terminal Railway
- Brownsville and Rio Grande International Railroad
- Buckingham Branch Railroad
- Buffalo and Pittsburgh Railroad
- Buffalo Southern Railroad
- Burlington Junction Railway
- C&S Railroad
- ← Panther Valley Railroad (-1990)
- ← Mahanoy and Hazleton Railway (-1987)
- ← Panther Valley Railroad (-1990)
- Caddo, Antoine and Little Missouri Railroad
- Cairo Terminal Railroad
- Caldwell County Railroad
- ← Carolina and Northwestern Railroad (-1994)
- California Northern Railroad
- California Western Railroad
- Camas Prairie Railroad
- Camp Chase Industrial Railroad
- Canada and Gulf Terminal Railway
- Canadian American Railroad
- Caney Fork and Western Railroad
- Canton Railroad
- ← Baltimore and Annapolis Railroad (-1991)
- Cape Breton and Central Nova Scotia Railway
- Cape Fear Railways
- Carolina Coastal Railway
- Carolina Piedmont Division, South Carolina Central Railroad
- Carolina Rail Services, Inc.
- Carolina Southern Railroad
- ← Mid Atlantic Railroad (1987-1995)
- Cartier Railway
- Cedar Rapids and Iowa City Railway
- Cedar River Railroad
- ← Cedar Valley Railroad (-1991)
- CenTex Rail Link
- ← Texas Central Railroad (-1994)
- Central California Traction Company
- Central Indiana and Western Railroad
- Central Kansas Railway
- Central Michigan Railway
- Central Montana Rail, Inc.
- Central New England Railroad
- Central of Tennessee Railway and Navigation Company
- Central Oregon and Pacific Railroad
- Central Railroad of Indiana
- Central Railroad of Indianapolis
- Central Western Railway
- Champagne Railroad
- ← Bath and Hammondsport Railroad (-1993)
- Chattahoochee Industrial Railroad
- Chattooga and Chickamauga Railway
- Cheney Railroad
- Chesapeake Railroad
- Chesapeake and Albemarle Railroad
- Chesapeake Western Railway
- Chestnut Ridge Railway
- Chicago and Illinois Midland Railway
- Chicago, Central and Pacific Railroad
- Chicago-Chemung Railroad
- Chicago Rail Link
- Chicago Short Line Railway
- Chicago SouthShore and South Bend Railroad
- Chicago, West Pullman and Southern Railroad
- Chillicothe-Brunswick Rail Maintenance Authority
- ← Wabash and Grand River Railway (-1993)
- ← Chillicothe-Brunswick Rail Maintenance Authority (-1990)
- ← Chillicothe Southern Railroad (-1987)
- ← Northern Missouri Railroad (1984-1986)
- ← Chillicothe Southern Railroad (-1987)
- ← Chillicothe-Brunswick Rail Maintenance Authority (-1990)
- ← Wabash and Grand River Railway (-1993)
- Cincinnati Terminal Railway
- City of Prineville Railway
- Claremont Concord Railroad
- Clarendon and Pittsford Railroad
- Clinton Terminal Railroad
- Coe Rail, Inc.
- Colonel's Island Railroad
- Colorado and Wyoming Railway
- Columbia and Cowlitz Railway
- Columbia Terminal
- Columbus and Greenville Railway
- Columbus and Ohio River Railroad
- Commonwealth Railway
- Conemaugh and Black Lick Railroad
- Connecticut Central Railroad
- Copper Basin Railway
- Council Bluffs Railway
- ← Council Bluffs and Ottumwa Railway (-1991)
- ← Iowa Southern Railroad (-1990)
- ← Ottumwa Terminal Railroad (-1989)
- ← Ottumwa Connecting Railroad (-1985)
- ← Council Bluffs and Ottumwa Railway (-1991)
- Crab Orchard and Egyptian Railroad
- Cuyahoga Valley Railway
- D&I Railroad
- Dakota, Minnesota and Eastern Railroad
- Dakota, Missouri Valley and Western Railroad
- Dakota Rail, Inc.
- Dakota Southern Railway
- Dallas, Garland and Northeastern Railroad
- Danbury Terminal Railroad
- Dansville and Mount Morris Railroad
- Dardanelle and Russelville Railroad
- Decatur Junction Railway
- Delaware-Lackawanna Railroad
- ← Lackawanna Railway (-1993)
- ← Lackawanna Valley Railroad (-1993)
- ← Pocono Northeast Railway (-1993)
- Delaware Valley Railway
- ← Octoraro Railway (-1994)
- Delray Connecting Railroad
- Delta Southern Railroad
- Delta Valley and Southern Railway
- Denver Rock Island Railroad
- ← Platt[sic] Valley Railway
- ← Denver Terminal Railroad (1993-1994)
- ← Denver Railway
- ← Denver Terminal Railway (-1989)
- ← Denver Railway
- ← Denver Terminal Railroad (1993-1994)
- ← Platt[sic] Valley Railway
- Depew, Lancaster and Western Railroad
- De Queen and Eastern Railroad
- Devco Railway
- Dodge City, Ford and Bucklin Railroad
- Doniphan, Kensett and Searcy Railway
- Dubois County Railroad
- Duluth and Northeastern Railroad
- Duluth, Missabe and Iron Range Railway
- Dunn-Erwin Railway
- Durham Transport, Inc.
- East Camden and Highland Railroad
- East Cooper and Berkeley Railroad
- East Erie Commercial Railroad
- East Jersey Railroad and Terminal Company
- East Mahanoy and Hazleton Railroad
- East Penn Railways
- East Portland Traction Company
- East Tennessee Railway
- Eastern Alabama Railway
- Eastern Idaho Railroad
- Eastern Illinois Railroad
- Eastern Maine Railway
- Eastern Shore Railroad
- EASX Railroad
- El Dorado and Wesson Railway
- Elgin, Joliet and Eastern Railway
- Ellis and Eastern Company
- Escanaba and Lake Superior Railroad
- Esquimalt and Nanaimo Railway
- Essex Terminal Railway
- Everett Railroad
- Farmrail Corporation
- Finger Lakes Railway
- Florida Central Railroad
- Florida East Coast Railway
- Florida Midland Railroad
- Florida Northern Railroad
- Florida West Coast Railroad
- Fordyce and Princeton Railroad
- Fort Smith Railroad
- Fort Worth and Dallas Railroad
- Fort Worth and Dallas Belt Railroad
- Fort Worth and Western Railroad
- Fox Valley and Western Ltd.
- ← Fox River Valley Railroad (-1993)
- ← Green Bay and Western Railroad (-1993)
- Fremont and Elkhorn Valley Railroad
- Galveston Railroad
- ← Galveston Wharves (-1987)
- Garden City Western Railway
- ← Garden City Northern Railroad (-1991)
- Gateway Eastern Railway
- Gateway Western Railway
- ← Kansas City Terminal Railway (-1994)
- Genesee and Wyoming Railroad
- Georgetown Railroad
- ← Belton Railroad (-1991)
- Georgia and Alabama Division, South Carolina Central Railroad
- Georgia and Florida Railroad
- Georgia Central Railway
- Georgia Northeastern Railroad
- Georgia Southwestern Division, South Carolina Central Railroad
- Georgia Woodlands Railroad
- Gettysburg Railroad
- Gloster Southern Railroad
- Goderich-Exeter Railway
- Golden Triangle Railroad
- Grafton and Upton Railroad
- Grainbelt Corporation
- Grand Forks Railway
- Grand Rapids Eastern Railroad
- Grand River Railway
- Greater Winnipeg Water District Railway
- Great Miami and Scioto Railway
- ← Indiana and Ohio Eastern Railroad (-1994)
- Great River Railroad
- Great Smoky Mountains Railway
- Great Walton Rail Company
- Great Western Railway (Colorado)
- Great Western Railway (Oregon)
- Green Mountain Railroad
- Greenville and Northern Railway
- Gulf, Colorado and San Saba Railway
- GWI Switching Services
- H&S Railroad
- ← Hartford and Slocomb Railroad (-1992)
- Hampton and Branchville Railroad
- ← Colleton County Railroad (1986-1988)
- Hampton Railway
- Harbor Belt Line Railroad
- Hardin Southern Railroad
- ← J&J Railroad (-1993)
- Hartwell Railroad
- High Point, Thomasville and Denton Railroad
- Hollidaysburg and Roaring Spring Railroad
- Hollis and Eastern Railroad
- Honey Creek Railroad
- Hoosier Southern Railroad
- Housatonic Railroad
- Huntsville and Madison County Railroad Authority
- ← Alabama Industrial Railroad (1984-1985)
- Huron and Eastern Railway
- Hutchinson and Northern Railway
- Idaho Northern and Pacific Railroad
- Indian Creek Railroad
- Indiana Rail Road
- Indiana and Ohio Railroad
- Indiana and Ohio Railway
- Indiana and Ohio Central Railroad
- Indiana Hi-Rail Corporation
- ← Spencerville and Elgin Railroad (-1991)
- Indiana Northeastern Railroad
- ← Hillsdale County Railway (-1992)
- ← Pigeon River Railroad (-1992)
- Indiana Southern Railroad
- Iowa Interstate Railroad
- Iowa Northern Railway
- Iowa Traction Railroad
- ISS Rail, Inc.
- JK Lines
- Jaxport Terminal Railway
- Jefferson Warrior Railroad
- Joppa and Eastern Railroad
- Juniata Terminal Company
- Kankakee, Beaverville and Southern Railroad
- Kansas Southwestern Railway
- Kentucky and Tennessee Railway
- Keokuk Junction Railway
- Kiamichi Railroad
- Kiski Junction Railroad
- Knox and Kane Railroad
- KWT Railway
- Kyle Railroad
- Lake Erie and Northern Railway
- Lake State Railway
- ← Detroit and Mackinac Railway (-1992)
- Lake Superior and Ishpeming Railroad
- Lake Terminal Railroad
- Lamoille Valley Railroad
- Twin State Railroad
- Lancaster and Chester Railway
- Lancaster Northern Railway
- Landisville Railroad
- Laurinburg and Southern Railroad
- Leadville-Climax Shortline Railway
- Lewis and Clark Railway
- ← Chelatchie Prairie Railroad (1981-1985)
- Little Kanawha River Railroad
- Little Rock and Western Railway
- Little Rock Port Railroad
- Live Oak, Perry and Georgia Railroad
- Livonia, Avon and Lakeville Railroad
- Logansport and Eel River Short Line Company
- Longview, Portland and Northern Railway
- Los Angeles Junction Railway
- Louisiana and Delta Railroad
- Louisiana and North West Railroad
- Louisville and Indiana Railroad
- Louisville, New Albany and Corydon Railroad
- Lowville and Beaver River Railroad
- Luzerne and Susquehanna Railway
- Madison Railroad
- Magma Arizona Railroad
- Mahoning Valley Railway
- Maine Coast Railroad
- Manufacturers Railroad
- Manufacturers Junction Railway
- Maryland and Delaware Railroad
- Maryland and Pennsylvania Railroad
- Maryland Midland Railway
- Massachusetts Central Railroad
- Massena Terminal Railroad
- McCloud Railway
- ← McCloud River Railroad (-1992)
- McKeesport Connectng Railroad
- McLaughlin Line Railroad
- Meridian and Bigbee Railroad
- MG Rail, Inc.
- Michigan Shore Railroad
- Michigan Southern Railroad
- Mid-Michigan Railroad
- Middletown and Hummelstown Railroad
- Middletown and New Jersey Railway
- Midland Terminal Company
- Midwest Coal Handling Company
- Milford-Bennington Railroad
- Minnesota Central Railroad
- Minnesota Commercial Railway
- ← Minnesota Transfer Railway (-1987)
- Minnesota, Dakota and Western Railway
- Mississippi and Skuna Valley Railroad
- Mississippi Central Railroad (1993-)
- ← Natchez Trace Railroad (-1993)
- Mississippi Delta Railroad
- Mississippi Export Railroad
- Mississippian Railway Cooperative
- ← Mississippian Railway (-1986)
- Missouri and Northern Arkansas Railroad
- Modesto and Empire Traction Company
- Mohawk, Adirondack and Northern Railroad
- Molalla Western Railway
- Monongahela Connecting Railroad
- Montana Rail Link
- Montana Western Railway
- Morristown and Erie Railway
- Moscow, Camden and San Augustine Railroad
- Mount Hood Railroad
- Mount Vernon Terminal Railway
- Mountain Laurel Railroad
- Municipality of East Troy, Wisconsin
- Napa Valley Railroad
- Nash County Railroad
- Nashville and Eastern Railroad
- Nebkota Railway
- Nebraska Central Railroad
- New Brunswick Southern Railway
- New England Central Railroad
- New England Southern Railroad
- New Hampshire and Vermont Railroad
- New Hampshire Central Railroad
- ← North Stratford Railroad (-1989)
- New Hampshire Northcoast Corporation
- New Hope and Ivyland Railroad
- New Orleans Lower Coast Railroad
- New Orleans Public Belt Railroad
- New York and Lake Erie Railroad
- New York Cross Harbor Railroad Terminal Corporation
- New York, Susquehanna and Western Railway
- Newburgh and South Shore Railroad
- Nimishillen and Tuscarawas Railway
- Nittany and Bald Eagle Railroad
- Nobles Rock Railroad
- ← Buffalo Ridge Railroad (-1992)
- Norfolk and Portsmouth Belt Line Railroad
- North Carolina and Virginia Railroad
- North Coast Railroad
- ← Arcata and Mad River Railroad (-1985)
- ← Eureka Southern Railroad (-1992)
- North Shore Railroad
- Northampton Switching Company
- Northeast Kansas and Missouri Division, Mid-Michigan Railroad
- Northern Nevada Railroad (1995-)
- ← Nevada Northern Railway (-about 1983)
- Northern Ohio and Western Railway
- Northwestern Oklahoma Railroad
- Oakland Terminal Railway
- Ogeechee Railway
- Ohi-Rail Corporation
- Ohio and Pennsylvania Railroad
- ← PL&W Railroad (-1995)
- ← Youngstown and Southern Railway (-1993)
- ← PL&W Railroad (-1995)
- Ohio Central Railroad
- Ohio Southern Railroad
- ← Moxahala Valley Railway (-1986)
- Oil Creek and Titusville Lines, Inc.
- Old Augusta Railroad
- Omaha, Lincoln and Beatrice Railway
- Ontario Central Railroad
- Ontario Midland Railroad
- Ontario Northland Railway
- Orange Port Terminal Railway
- Osage Railroad
- Otter Tail Valley Railroad
- Ouachita Railroad
- Owego and Harford Railway
- ← Tioga Central Railroad (-1992)
- Paducah and Louisville Railway
- Palouse River Railway
- Panhandle and Northern Railroad
- Parr Terminal Railroad
- Patapsco and Back Rivers Railroad
- Pearl River Valley Railroad
- Pecos Valley Southern Railway
- Pee Dee River Railway Division, Aberdeen and Rockfish Railroad
- Pend Oreille Valley Railroad
- Peninsula Terminal Company
- Peoria and Pekin Union Railway
- Pickens Railroad
- Pigeon River Railroad
- Pine Belt Southern Railroad
- Pioneer Valley Railroad
- Pittsburg and Shawmut Railroad
- Pittsburgh, Allegheny and McKees Rocks Railroad
- Plainview Terminal Company
- Point Comfort and Northern Railway
- Port Bienville Railroad
- Port Jersey Railroad
- Portland and Western Railroad
- Port of Palm Beach District Railway
- Port of Tillamook Bay Railroad
- Port Railroads, Inc.
- Port Royal Railroad
- Port Terminal Railroad of South Carolina
- Port Utilities Commission of Charleston, South Carolina
- Poseyville and Owensville Railroad
- Prescott and Northwestern Railroad
- Providence and Worcester Railroad
- ← Connecticut Rail Systems (-1993)
- Quebec North Shore and Labrador Railway
- Quebec Railway Corporation (Charlevoix Railway)
- Quincy Railroad
- Quincy Bay Terminal Company
- ← Fore River Railway (-1992)
- Railroad Switching Service of Missouri
- Rarus Railway
- ← Butte, Anaconda and Pacific Railway (-1985)
- Reading Blue Mountain and Northern Railroad
- Red Bank Railroad
- Red River Valley and Western Railroad
- Red Springs and Northern Railroad
- Redmont Railway
- Rio Valley Switching Company
- River Terminal Railway
- R.J. Corman Railroad
- R.J. Corman Railroad/Cleveland Line
- R.J. Corman Railroad/Memphis Line
- R.J. Corman Railroad/Western Ohio Line
- Roberval and Saguenay Railway
- Rochester and Southern Railroad
- Rockdale, Sandow and Southern Railroad
- Roscoe, Snyder and Pacific Railway
- Sabine River and Northern Railroad
- Saginaw Valley Railway
- St. Lawrence and Atlantic Railroad
- St. Lawrence and Raquette River Railroad
- ← St. Lawrence Railroad (-1990)
- St. Maries River Railroad
- St. Marys Railroad
- Salt Lake City Southern Railroad
- Salt Lake, Garfield and Western Railway
- San Diego and Imperial Valley Railroad
- San Joaquin Valley Railroad
- San Luis Central Railroad
- San Manuel Arizona Railroad
- San Pedro and Southwestern Railway
- Sand Springs Railway
- Sandersville Railroad
- Santa Cruz, Big Trees and Pacific Railway
- Santa Fe Southern Railway
- Santa Maria Valley Railroad
- Savannah State Docks Railroad
- Seminole Gulf Railway
- Semo Port Railroad
- Sequatchie Valley Railroad
- SF&L Railway
- Shamokin Valley Railroad
- Shenandoah Valley Railroad
- Sidney and Lowe Railroad
- Sierra Railroad
- Sisseton Milbank Railroad
- ← Sisseton Southern Railroad (-1989)
- SMS Rail Service, Inc.
- Somerset Railroad
- South Branch Valley Railroad
- South Brooklyn Railway
- South Buffalo Railway
- South Carolina Central Railroad
- South Central Florida Express, Inc.
- ← South Central Florida Railroad (-1994)
- South Central Tennessee Railroad
- South Kansas and Oklahoma Railroad
- South Orient Railroad
- South Plains Lamesa Railroad
- Southeast Kansas Railroad
- Southeastern International Corporation
- Southern Alabama Railroad
- Southern Indiana Railway
- Southern Railroad of New Jersey
- ← Shore Fast Line, Inc. (-1991)
- ← West Jersey Railroad (1990-1995)
- ← Pioneer Railroad, West Jersey Division (-1990)
- ← West Jersey Short Line (-1988)
- ← Pioneer Railroad, West Jersey Division (-1990)
- Southern Railway of British Columbia
- Southern Switching Company
- Southwest Pennsylvania Railroad
- Southwestern Railroad
- ST Rail System
- State University Railroad
- Steelton and Highspire Railroad
- Sterling Belt Line Railroad
- Stewartstown Railroad
- Stockton Terminal and Eastern Railroad
- Stourbridge Railroad
- ← Lackawaxen and Stourbridge Railroad (-1989)
- Strasburg Rail Road
- Tacoma Eastern Railway
- Tacoma Municipal Belt Line Railway
- ← Port of Tacoma (-1984)
- Tennessee Railway
- Tennessee Southern Railroad
- Tennken Railroad
- Terminal Railway, Alabama State Docks
- Texas – New Mexico Railroad
- Texas and Northern Railway
- Texas and Oklahoma Railroad
- Texas City Terminal Railway
- Texas, Gonzales and Northern Railway
- Texas Mexican Railway
- Texas North Western Railway
- Texas Northeastern Division, Mid-Michigan Railroad
- Texas, Oklahoma and Eastern Railroad
- Texas South-Eastern Railroad
- Texas Transportation Company
- Thermal Belt Railway
- Toledo, Peoria and Western Railway
- Tomahawk Railway
- ← Marinette, Tomahawk and Western Railroad (-1991)
- Tonawanda Island Railroad
- Toppenish, Simcoe and Western Railroad
- Towanda-Monroeton Shippers Lifeline, Inc.
- Transkentucky Transportation Railroad
- Trinidad Railway
- Trona Railway
- Tulare Valley Railroad
- ← Sunset Railway (-1992)
- Tulsa-Sapulpa Union Railway
- Turtle Creek Industrial Railroad
- Tuscola and Saginaw Bay Railway
- Twin Cities and Western Railroad
- Tyburn Railroad
- Tyson Railroad
- Union County Industrial Railroad
- Union Railroad
- Upper Merion and Plymouth Railroad
- Utah Central Railway
- Utah Railway
- Valdosta Railway
- Vandalia Railroad
- Ventura County Railway
- Vermont Railway
- Virginia Southern Division, North Carolina and Virginia Railroad
- Wabush Lake Railway, Arnaud Railway
- Waccamaw Coast Line Railroad
- ← Horry County Railway (-1987)
- Walkersville Southern Railroad
- Walking Horse and Eastern Railroad
- Warren and Saline River Railroad
- ← Warren and Ouachita Valley Railway (-1980)
- Warren and Trumbull Railroad
- Washington Central Railroad
- Washington County Railroad
- Waterloo Railway
- WCTU Railway
- Wellsboro and Corning Railroad
- West Michigan Railroad
- West Tennessee Railroad
- West Texas and Lubbock Railroad
- Western Kentucky Railway
- ← Tradewater Railway (-1995)
- Western Plant Services, Inc.
- Western Rail Road
- Wheeling and Lake Erie Railway
- White Pass and Yukon Corporation (-1985, still operates tourist trains)
- Wichita, Tillman and Jackson Railway
- Willamette and Pacific Railroad
- Willamette Valley Railway
- Willamina and Grand Ronde Railway
- Wilmington and Western Railroad
- Winamac Southern Railway
- Winchester and Western Railroad (Virginia Division)
- Winchester and Western Railroad (New Jersey Division)
- ← Jersey Southern Railway (-1987)
- Windsor and Hantsport Railway
- Winston-Salem Southbound Railway
- Wiregrass Central Railroad
- Wisconsin and Calumet Railroad
- Wisconsin and Southern Railroad
- Wisconsin Central Ltd.
- Wye Transportation Corporation
- Wyoming Colorado Railroad
- Wyoming Colorado Railroad (Oregon Eastern Division)
- ← Oregon and Northwestern Railroad (-1990)
- Yadkin Valley Railroad
- Yolo Shortline Railroad
- Yorkrail, Inc.
- Youngstown and Austintown Railroad
- Yreka Western Railroad
- Non-operating
- Buffalo Creek Railroad
- ← Buffalo Creek and Gauley Railroad (-1991)
- Central New York Railroad
- Elk River Railroad
- KCT Railway
- Leelanau Transit Company
- Louisville and Wadley Railway
- Ludington and Northern Railway
- Olympic Railroad
- Southern San Luis Valley Railroad
- Tucson, Cornelia and Gila Bend Railroad
- Winifrede Railroad
- Class I predecessors
- Burlington Northern Railroad
- Conrail
- ← Monongahela Railway (-1993)
- CSX Transportation
- ← Pittsburgh and Ohio Valley Railway (-1993)
- ← Port Huron and Detroit Railroad (-1989)
- ← Strouds Creek and Muddlety Railroad (-1992)
- ← Three Rivers Railway (-1993)
- ← Pittsburgh and Lake Erie Railroad (-1992)
- Kansas City Southern Railway
- ← Arkansas Western Railway (-1992)
- ← Fort Smith and Van Buren Railroad (-1992)
- ← Graysonia, Nashville and Ashdown Railroad (-1993)
- ← MidLouisiana Rail Corporation (-1994)
- ← Central Louisiana and Gulf Railroad (1981-1987)
- ← North Louisiana and Gulf Railroad (-1987)
- ← MidSouth Rail Corporation (-1994)
- ← SouthRail (-1994)
- ← TennRail (-1994)
- ← Corinth and Counce Railroad (-1991)
- Norfolk Southern Railway
- ← Georgia Northern Railroad (-1994)
- ← Interstate Railroad (-1992)
- ← Live Oak, Perry and South Georgia Railway (-1994)
- ← Norfolk and Western Railway
- ← Des Moines Union Railway (-1989)
- ← Tennessee, Alabama and Georgia Railway (-1991)
- Soo Line Railroad
- Southern Pacific Transportation Company
- ← Holton Interurban Railway (-1985)
- ← Visalia Electric Railroad (-1992)
- Union Pacific Railroad
- ← Missouri Pacific Railroad
- ← Galveston, Houston and Henderson Railroad (-1989)
- ← Great Southwest Railroad (-1986)
- ← Texas-New Mexico Railway (-1977)
- ← Weatherford, Mineral Wells and Northwestern Railway (-1988)
- ← Sacramento Northern Railway (-1988)
- ← Tidewater Southern Railway (-1988)
- ← Missouri Pacific Railroad
- Split
- Chicago, Missouri and Western Railway (-1990) → SPCSL Corporation (1989), Gateway Western Railway (1990)
- Colorado and Eastern Railroad (-1987)
- Columbia and Silver Creek Railroad (-about 1992) → Gloster Southern Railroad (1988)
- Davenport, Rock Island and North Western Railway (-1995) → Burlington Northern Railroad, Soo Line Railroad
- Indiana Midland Railway (-1987) → Carthage, Knightstown and Shirley Railroad (not split, but CK&S is not listed)
- Iowa Railroad (1981-1987) → Iowa Interstate Railroad (1994)
- Iowa Terminal Railroad (-1987) → Cedar Valley Railroad (1986), Iowa Traction Railroad (1987)
- North Carolina Ports Commission (-1986) → Wilmington Terminal Company, Carolina Rail Service
- North Central Oklahoma Railroad (1982-1985) → Oklahoma, Kansas and Texas Railroad (1982), AT&L Railroad (1985)
- North Central Oklahoma and Midlands Railway (about 1988, never operated)
- Northwestern Pacific Railroad (-1992) → Eureka Southern Railroad (1984), Southern Pacific Transportation Company (1992)
- Abandoned
- Ahnapee and Western Railway (-1990, no longer a common carrier)
- Alabama Southern Railroad (1985-1985, never operated)
- ← Sumter and Choctaw Railroad (-1986)
- Borinstein Railroad (-about 1994)
- Brillion and Forest Junction Railroad (1978-1985)
- C&J Railroad (-1987)
- Cadillac and Lake City Railway (-1989)
- Cadiz Railroad (-1988)
- Cambria and Indiana Railroad (-1994)
- Camino, Placerville and Lake Tahoe Railroad (-1986)
- Central Wisconsin Railroad (-1985)
- Chaparral Railroad (-1995)
- Charles City Rail Lines (-1994)
- Chattahoochee Valley Railway (-1992)
- Cimarron River Valley Railway (-1989)
- Cliffside Railroad (-1992)
- Cooperstown and Charlotte Valley Railway (-1995)
- Crosbyton Railroad (1990-1990)
- Crystal City Railroad (-1995)
- Curtis, Milburn and Eastern Railroad (-1993)
- Elkhorn and Walworth Railroad (-1985)
- Fairmont and Western Railroad (-1988)
- Falls Creek Railroad (-1988)
- Ferdinand and Huntingburg Railroad (-1991)
- ← Ferdinand Railroad (-1987)
- Fonda, Johnstown and Gloversville Railroad (-1988)
- Frankfort and Cincinnati Railroad (-1985)
- Franklin County Railroad (1985-1988)
- Fulton County Railroad (1981-1985)
- Georgia Great Southern Division, South Carolina Central Railroad (-1994)
- Golden Cat Railroad (-1993)
- Graham County Railroad (-1987)
- Helena Southwestern Railroad (-1987)
- Hillsboro and North Eastern Railway (-1987)
- Iron Cliffs Railway (1991-1991)
- Jackson and Southern Railroad (-1992)
- Kansas City Public Service Freight Operations (-1981, -1989)
- Kewash Railroad (-1988)
- Kelly's Creek and Northwestern Railroad (-1995)
- Klamath Northern Railway (-1991)
- Lake Erie, Franklin and Clarion Railroad (-1992)
- Lakeside Transportation (1993-1994)
- Laona and Northern Railway (-1983)
- Lenawee County Railroad (-1990)
- Louisiana Midland Railway (-1986)
- Marion County Railway (1984-1985)
- Marquette and Huron Mountain Railroad (-1985)
- Mercersburg Railway (1978-1980)
- Michigan Northern Railway (-1986)
- Mineral Wells and Eastern Railway (-1992)
- Montour Railroad (-1986)
- Muncie and Western Railroad (-1995)
- Nicolet Badger Northern Railroad (-1995)
- Oklahoma Central Railroad (-1988)
- Oregon, California and Eastern Railway (-1990)
- Oregon, Pacific and Eastern Railway (-1994)
- Petaluma and Santa Rosa Railroad (-1985)
- Pioneer and Fayette Railroad (-about 1991)
- Portland Traction Company (-1990)
- Prairie Trunk Railway (-1985)
- Rahway Valley Railroad (-1992)
- Rocky Mountain Railcar and Railroad (-1995)
- Saltville Railroad (-1991)
- San Francisco Belt Railroad (-1993)
- Staten Island Railway (-1992)
- Sugar Loaf and Hazleton Railroad (-1989)
- T&P Railway (1991-1993)
- Terre Haute, Brazil and Eastern Railroad (-1993)
- Union Railroad of Oregon (-1995)
- Unity Railways (-1994)
- Wabash and Erie Railroad (1994, rejected by ICC)
- Wabash and Ohio River Railroad (1994, rejected by ICC)
- Ware Shoals Railroad (-1985)
- Warrenton Railroad (-1985)
- West Virginia Northern Railroad (-1993)
- Willamette Valley Railroad (-1993)
- ← Valley and Siletz Railroad (-1985)
- ← Willamina and Grand Ronde Railroad (-1986)
- Wisconsin and Michigan Railway (-1995)
- Wisconsin Western Railroad (1982-1985)
- Wolfeboro Railroad (-1986)
- Wyandotte Southern Railroad (-1986)
- Yakima Valley Transportation Company (-1985)
- Yancey Railroad (-1985)