User:ManVhv/Sandbox/EBOV Proteins

The genome is non-segmented negative-sense RNA (nsnsRNA).

The gene order is 3′ – leader – NP – VP35 – VP40 – GP/sGP – VP30 – VP24 – L – trailer – 5′

  • NP
  • VP35
  • VP40
  • GP/sGP
  • VP30
  • VP24
  • L - "Large" polymerase - complex with VP35 constitutes RNA-dependent RNA polymerase (RdRp)
  • PMID 10073695
  • Determined Nucletoide sequence of L encoding gene and 5' end of RNA.
  • RNA has some folded structure, with self-complementary regions on 5' end, and 3' is complemetary to 5'.
  • L protein has conserved amino acid sequences with other nsnsRNA viruses
  • Conserved functionality is RNA-dependent RNA polymerase (RdRp)
  • VSV L protein is catalyst for 5'-end capping
  • VSV mRNA is capped with GDP instead of GTP
  • VSV L caps mRNA via "RNA:GDP polyribonucleotidyltransferase activity, in which a 5'-monophosphorylated viral mRNA-start sequence is transferred to GDP generated from GTP via a covalent enzyme-RNA intermediate"
NP VP35 VP40 GP/sGP VP30 VP24 L
NP nucleocapsid nucleocapsid nucleocapsid
VP35 RdRp RdRp
VP40
GP/sGP
VP30 nucleocapsid RdRp
VP24
L
EBOV dsRNA X
EBOV mRNA capping 5' with GDP
NPC-1 entry