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Alkaline Phosphatase is a Zinc containing dimeric enzyme with the MW: 86,000 kDa. It is heat stable and its function is to remove phosphate groups from phosphorylated compounds facilitating transport across membranes and providing the cell with a source of inorganic phosphate. Alkaline Phosphatase in E.coli is located in the periplasmic space and we can thus, selectively release it with a technique that weakens the cell wall and releases the proteins. Due to the location of the enzyme, and the protein layout of the enzyme the enzyme is in solution with a small amount of proteins than there are in another portion of the cell.