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In the laboratory, the strength of this interaction can be measured by finding the temperature necessary to break the hydrogen bonds, their melting temperature (also called Tm value). "For a sequence of 14 nucleotides or less Tm (degrees C) = (2*A+T)+(4*G+C) is used to calculate melting temperature. If the sequence is greater than 13 nucleotides Tm (degrees C) = 64.9+41*(G+C-16.4)/(A+T+G+C) is used to calculate melting temperature." When all the base pairs in a DNA double helix melt, the strands separate and exist in solution as two entirely independent molecules.
notes
edit- ^ "Melting Temperature (Tm) Calculation". biophp.org. Retrieved 04-25-17.
{{cite web}}: Check date values in:|accessdate=(help) - ^ "Basic Melting Temperature (Tm) Calculations". http://biotools.nubic.northwestern.edu. Retrieved 4-25-17.
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