Binding edit
The initiation of the transcription if a multistep sequential process that involves several mechanisms: promoter location, initial reversible binding of RNA polymerase, conformational changes in RNA polymerase, conformational changes in DNA, binding of nucleoside triphosphate (NTP) to the functional RNA polymerase-promoter complex, and nonproductive and productive initiation of RNA synthesis[1].
The promoter binding process is crucial in the understanding of the process of gene expression.
Promoter Location edit
Although RNA polymerase holoenzyme shows high affinity to non-specific sites of the DNA, this characteristic does not allow us to clarify the process of promoter location[2]. This process of promoter location has been attributed to the structure of the holoenzyme to DNA and sigma 4 to DNA complexes[3].
References edit
- ^ DeHaseth, P.L.; Zupancic,, M.; Recor, T.M. (1998). "RNA Polymerase-Promoter Interactions: the Comings and Goings of RNA Polymerase". Journal of Bacteriology. 180: 3019-3025.
{{cite journal}}: line feed character in|title=at position 54 (help)CS1 maint: extra punctuation (link) - ^ Singer, P.; Wu, C.W. (1987). "Promoter Search by Escherichia coli RNA Polymerase on a Circular DNA Template". The Journal of Biological Chemistry. 262: 14178–14189.
- ^ Borukhov, S.; Nudler, E. (2003). "RNA polymerase holoenzyme: structure, function and
biological implications". Current Opinion in Microbiology. 6: 93–100.
{{cite journal}}: line feed character in|title=at position 51 (help)