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editIn a 2015 study on the role of N6-methyladenosine (m6A) demethylation on adipogenesis, researchers treated porcine adipocytes with increasing concentrations of cycloleucine. The researchers measured mRNA concentration of m6A using the Dot Blot method, and the results showed that cycloleucine increased adipocyte growth by blocking methylation by inhibiting m6A levels relative to the control adipocytes.[1]
Building on the findings on cycloleucine’s role in the inhibition of N6-methyladenosine activity a 2022 study showed that cycloleucine inhibits porcine oocyte and embryo development. Researchers cultured porcine oocytes and isolated cumulus cells from their cumulus cell complexes, after which the cells were cultured in vitro with cycloleucine in increasing concentrations.The samples were monitored under fluorescence microscopy, with the results showing positive relationship between cycloleucine concentration and decreased viability and survival rate for oocytes.[2]
References
edit- ^ Wang, Xinxia; Zhu, Linna; Chen, Jingqing; Wang, Yizhen (2015-04-03). "mRNA m6A methylation downregulates adipogenesis in porcine adipocytes". Biochemical and Biophysical Research Communications. 459 (2): 201–207. doi:10.1016/j.bbrc.2015.02.048. ISSN 0006-291X.
- ^ Zhang, Meng; Zhang, Sheng; Zhai, Yanhui; Han, Yu; Huang, Rong; An, Xinglan; Dai, Xiangpeng; Li, Ziyi (2022-02-01). "Cycloleucine negatively regulates porcine oocyte maturation and embryo development by modulating N6-methyladenosine and histone modifications". Theriogenology. 179: 128–140. doi:10.1016/j.theriogenology.2021.11.024. ISSN 0093-691X.