Dda
editDda (also known as DNA-dependent ATPase, Dda helicase, Dda DNA helicase) is the 49,897 Dalton 439 amino acid polypeptide coded by the Dda gene of the Enterobacteria phage T4.
Biochemistry
editDda hydrolyzes ATP in and only in the presence of substrate single stranded DNA. Dda binds its substrate (ssDNA) and processively translocates in a 5' to 3' direction in an ATP-dependent manner. In other words, Dda couples ATP hydrolysis and ssDNA translocation.
Coding Nucleotide Sequence[1]
31,319 bases (32kb) from base 9410 to 10729
ATGACATTTGATGATTTGACCGAAGGCCAGAAAAATGCCTTTAACATTGTTATGAAGGCTATTAAAGAAA
AGAAACATCATGTAACTATTAATGGACCTGCTGGTACCGGTAAGACTACTCTTACTAAGTTCATCATTGA
AGCTTTAATATCTACGGGTGGAACTGGTATTATTTTAGCAGCTCCTACACATGCAGCTAAAAAGATTCTT
TCAAAACTATCAGGGAAAGAAGCGAGTACTATTCATAGTATTCTTAAAATTAACCCAGTAACATATGAAG
AAAATGTTCTTTTTGAACAAAAAGAAGTACCTGATTTAGCCAAATGCAGAGTATTAATCTGCGACGAAGT
GTCAATGTATGATAGAAAGCTATTTAAAATTCTGCTTTCAACTATTCCACCTTGGTGTACTATAATTGGA
ATAGGGGATAATAAGCAAATCAGACCTGTTGAACCAGGAGAAAATACTGCTTATATCAGTCCATTCTTTA
CACATAAAGATTTTTATCAGTGTGAACTCACTGAAGTTAAACGCAGTAATGCTCCTATTATTGATGTAGC
TACTGACGTTCGCAACGGTAAGTGGAATTATGATAAAGTTGTTGACGGGCATGGAGTACGTGGATTTACT
GGTGATACCGCTTTACGCGATTTTATGGTAAATTATTTTTCAATCGTCAAATCACTAGATGATTTGTTTG
AAAATCGCGTAATGGCATTTACGAATAAATCTGTTGACAAGTTAAATAGCATTATTCGTAAAAAGATTTT
TGAAACTGATAAAGATTTTATTGTCGGTGAAATTATTGTAATGCAGGAACCATTATTTAAAACATATAAA
ATTGATGGAAAGCCTGTGTCAGAAATTATTTTTAATAACGGACAATTAGTTCGTATTATAGAAGCAGAGT
ATACATCAACGTTTGTTAAAGCCCGTGGTGTTCCTGGAGAATATCTAATTCGTCATTGGGATTTAACAGT
AGAAACTTATGGCGATGATGAATATTATCGTGAAAAGATTAAAATAATTTCATCTGACGAAGAATTGTAT
AAGTTTAACCTATTTTTAGCTAAAACAGCAGAAACTTATAAAAATTGGAACAAAGGCGGAAAAGCTCCGT
GGAGTGATTTTTGGGATGCTAAATCACAGTTTAGTAAAGTGAAAGCACTTCCTGCATCAACATTCCATAA
AGCGCAGGGTATGTCTGTAGACCGTGCTTTCATTTATACGCCTTGTATTCATTATGCAGATGTTGAATTA
GCTCAACAACTTCTTTATGTTGGTGTCACCCGTGGTCGTTATGATGTGTTTTATGTATGA
Amino Acid Sequence[2]
MTFDDLTEGQ KNAFNIVMKA IKEKKHHVTI NGPAGTGKTT LTKFIIEALI STGGTGIILA APTHAAKKIL SKLSGKEAST IHSILKINPV TYEENVLFEQ KEVPDLAKCR VLICDEVSMY DRKLFKILLS TIPPWCTIIG IGDNKQIRPV EPGENTAYIS PFFTHKDFYQ CELTEVKRSN APIIDVATDV RNGKWNYDKV VDGHGVRGFT GDTALRDFMV NYFSIVKSLD DLFENRVMAF TNKSVDKLNS IIRKKIFETD KDFIVGEIIV MQEPLFKTYK IDGKPVSEII FNNGQLVRII EAEYTSTFVK ARGVPGEYLI RHWDLTVETY GDDEYYREKI KIISSDEELY KFNLFLAKTA ETYKNWNKGG KAPWSDFWDA KSQFSKVKAL PASTFHKAQG MSVDRAFIYT PCIHYADVEL AQQLLYVGVT RGRYDVFYV
Molecular Biology
editDda is involved in the initiation of T4 DNA replication and DNA recombination.
Cellular Biology
editDda is toxic to cells at elevated levels.