P ( n ≥ κ ) = 1 − ∑ x = 0 κ − 1 e − λ ⋅ λ x x ! {\displaystyle P\left(n\geq \kappa \right)=1-\sum _{x=0}^{\kappa -1}{\frac {e^{-\lambda }\cdot \lambda ^{x}}{x!}}} , where n {\displaystyle n} equals the number of solutions and κ {\displaystyle \kappa } equals actual number of occurrences. λ {\displaystyle \lambda } is the expected number of occurrences, which equals m ⋅ s p {\displaystyle {\frac {m\cdot s}{p}}} .
m {\displaystyle m} = number of plasmids, s {\displaystyle s} = number of solved permutations of edges, p {\displaystyle p} = number of permutations of edges.
