- West Group
| Pos | Team | Pld | W | PKW | PKL | L | GF | GA | GD | Pts | Qualification |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Union Omaha | 1 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 2 | Advance to knockout phase |
| 2 | Central Valley Fuego FC | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | |
| 3 | Northern Colorado Hailstorm FC | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 1 | |
| 4 | Spokane Velocity FC | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 |
Updated to match(es) played on May 1, 2024. Source: USL League One
- Central Group
| Pos | Team | Pld | W | PKW | PKL | L | GF | GA | GD | Pts | Qualification |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | One Knoxville SC | 1 | 1 | 0 | 0 | 0 | 1 | 0 | +1 | 3 | Advance to knockout phase |
| 2 | Lexington SC | 1 | 1 | 0 | 0 | 0 | 1 | 0 | +1 | 3 | |
| 3 | Forward Madison FC | 1 | 0 | 0 | 0 | 1 | 0 | 1 | −1 | 0 | |
| 4 | Chattanooga Red Wolves SC | 1 | 0 | 0 | 0 | 1 | 0 | 1 | −1 | 0 |
Updated to match(es) played on April 28, 2024. Source: USL League One
- East Group
| Pos | Team | Pld | W | PKW | PKL | L | GF | GA | GD | Pts | Qualification |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Charlotte Independence | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | Advance to knockout phase |
| 2 | Richmond Kickers | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | |
| 3 | Greenville Triumph SC | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | |
| 4 | South Georgia Tormenta FC | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 |
Updated to match(es) played on April 27, 2024. Source: USL League One