| Pos | Team | Pld | W | SOW | SOL | L | GF | GA | GD | Pts | Awards |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Philadelphia Union II | 9 | 5 | 2 | 1 | 1 | 18 | 9 | +9 | 20 | Regular season champion |
| 2 | Chattanooga FC | 10 | 5 | 2 | 1 | 2 | 19 | 12 | +7 | 20 | |
| 3 | FC Cincinnati 2 | 10 | 6 | 0 | 1 | 3 | 20 | 14 | +6 | 19 | |
| 4 | Ventura County FC | 9 | 5 | 2 | 0 | 2 | 18 | 11 | +7 | 19 | |
| 5 | North Texas SC | 9 | 4 | 3 | 1 | 1 | 12 | 7 | +5 | 19 | |
| 6 | Los Angeles FC 2 | 9 | 5 | 1 | 1 | 2 | 18 | 13 | +5 | 18 | |
| 7 | St. Louis City 2 | 9 | 5 | 1 | 1 | 2 | 12 | 9 | +3 | 18 | |
| 8 | Sporting Kansas City II | 10 | 5 | 0 | 2 | 3 | 22 | 16 | +6 | 17 | |
| 9 | New York Red Bulls II | 10 | 4 | 2 | 1 | 3 | 19 | 21 | −2 | 17 | |
| 10 | Columbus Crew 2 | 9 | 4 | 1 | 1 | 3 | 16 | 16 | 0 | 15 | |
| 11 | The Town FC | 9 | 3 | 2 | 2 | 2 | 10 | 8 | +2 | 15 | |
| 12 | Toronto FC II | 8 | 4 | 0 | 2 | 2 | 11 | 9 | +2 | 14 | |
| 13 | Tacoma Defiance | 9 | 4 | 1 | 0 | 4 | 17 | 19 | −2 | 14 | |
| 14 | Chicago Fire FC II | 8 | 3 | 2 | 1 | 2 | 17 | 15 | +2 | 14 | |
| 15 | Crown Legacy FC | 9 | 3 | 2 | 1 | 3 | 15 | 14 | +1 | 14 | |
| 16 | Portland Timbers 2 | 10 | 3 | 1 | 2 | 4 | 16 | 15 | +1 | 13 | |
| 17 | Orlando City B | 9 | 3 | 1 | 2 | 3 | 15 | 14 | +1 | 13 | |
| 18 | New York City FC II | 8 | 3 | 1 | 1 | 3 | 16 | 12 | +4 | 12 | |
| 19 | Inter Miami CF II | 9 | 3 | 0 | 2 | 4 | 16 | 20 | −4 | 11 | |
| 20 | New England Revolution II | 9 | 3 | 1 | 0 | 5 | 15 | 20 | −5 | 11 | |
| 21 | Atlanta United 2 | 9 | 3 | 1 | 0 | 5 | 12 | 18 | −6 | 11 | |
| 22 | Whitecaps FC 2 | 9 | 2 | 1 | 2 | 4 | 14 | 16 | −2 | 10 | |
| 23 | Austin FC II | 8 | 2 | 1 | 2 | 3 | 9 | 11 | −2 | 10 | |
| 24 | Minnesota United FC 2 | 7 | 3 | 0 | 0 | 4 | 12 | 16 | −4 | 9 | |
| 25 | Houston Dynamo 2 | 8 | 2 | 1 | 1 | 4 | 10 | 14 | −4 | 9 | |
| 26 | Real Monarchs | 9 | 2 | 1 | 0 | 6 | 9 | 16 | −7 | 8 | |
| 27 | Colorado Rapids 2 | 9 | 2 | 0 | 1 | 6 | 13 | 21 | −8 | 7 | |
| 28 | Carolina Core FC | 8 | 1 | 2 | 0 | 5 | 7 | 15 | −8 | 7 | |
| 29 | Huntsville City FC | 9 | 0 | 0 | 3 | 6 | 10 | 17 | −7 | 3 |
Updated to match(es) played on May 23, 2024. Source: MLSNP
Rules for classification: 1) points; 2) total wins; 3) total goal differential; 4) total goals scored; 5) fewer disciplinary points; 6) away goal differential; 7) away goals scored; 8) home goals differential; 9) home goals scored; 10) coin toss (2 clubs tied) or drawing of lots (≥3 clubs tied)
Rules for classification: 1) points; 2) total wins; 3) total goal differential; 4) total goals scored; 5) fewer disciplinary points; 6) away goal differential; 7) away goals scored; 8) home goals differential; 9) home goals scored; 10) coin toss (2 clubs tied) or drawing of lots (≥3 clubs tied)