Talk:Framework region

Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Bcern001. Peer reviewers: Bcern001.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 21:25, 17 January 2022 (UTC)

Edit Summary to Framework Region

Due to the complex wording and the absence of important details, I edited this article on the framework region so it would be less confusing and unclear to readers. I changed the wording of some phrases so they would be easier understood. For example I changed “protein which belongs to the immunoglobulin superfamily” to antibody- framework regions are only located on the variable regions of antibodies, and saying antibodies is much easier and less wordy than “protein which belongs to the immunoglobulin superfamily”. Also I explained its function and that the framework region is a part of the variable region (otherwise known as the fragment antigen binding region) because it's necessary in order to properly explain what the function of the framework region actually is. I also added a picture to describe their location. Lastly, citations were also added to the end of the article. Bcern001 (talk) 22:09, 17 November 2016 (UTC)

So I would consider adding a few specific examples or models of the Framework region and how it interacts with other antibodies and antigens specifically. Also, I would include more pictures of these interactions just to clarify the specifics on what you are talking about.Kmcki003 (talk) 16:09, 19 November 2016 (UTC)kmcki003

Okay awesome, I'll look into adding examples to clarify specifics... Thanks! Bcern001 (talk) 17:17, 20 November 2016 (UTC)

This article needs more clarification. It is initially unclear how the highly variable region is abbreviated as CDR where CDR is actually an abbreviation for complementarity determining regions and HV is hyper variable. Although not necessarily a dramatic change, I feel as though it is important to note these hyper variable regions can be referred to as such or as complementarity determining regions due to their direct interaction with the antigen but that CDR does not itself stand for a hyper variable region. In addition, the article is incorrect in saying that the variable region is the same as the Fab region, or seems to say that. The variable region is only a portion of the Fab region which is composed of both variable regions and conserved regions. I am going to work on the semantics and word choice in the page to make it more clear and to ensure the articles are presenting the correct information. I may create a new picture that shows the different compartments of the antibody which include the conserved and variable regions in the Fab to make it clear the Fab is not only the variable regions. This will also provide the reader with a better understanding of the antibody as a whole. Immcarle70 (talk) 20:52, 16 January 2018 (UTC) User: ImmCarle70 16 Jan, 2018 — Preceding unsigned comment added by Immcarle70 (talk • contribs) 20:46, 16 January 2018 (UTC)

https://www.ncbi.nlm.nih.gov/books/NBK27144/ A great resource that explains the different parts of the antibody and clearly defines the different regions of the antibody. The pictures it shows remain unclear though because they do not include all different aspects, allowing the reader to understand a full picture of what is going on. https://www.wiley.com/legacy/products/subject/life/elgert/CH04.pdf Contains much better pictures for the idea of how each portion of the antibody goes along with one another. It includes the V,D,J explanations and also the C regions on the antibody while simultaneously showing which portions are framework. It also has a great 3D picture which gives us a better idea of the 3 "Finger- like" projections on the antibody (resulting from the three main coding regions) making it more clear of how the antibody ends up folding and makes it clear which specific portions of the antibody end up binding to the antigen. Zhu K et al. Antibody structure determination using a combination of homology modeling, energy-based refinement, and loop prediction. Pro- teins 2014; 8: 1646–1655. This research experiment in 2004 used BioLuminate and Prime to predict second antibody modeling assessment to better understand the structures and locations of the CDR loops and H3 loops. This is a blinded approach for them to predict the CDR loops and where they are in the antibody itself. Brian D. Weitzner, Roland L. Dunbrack, Jeffrey J. Gray, The Origin of CDR H3 Structural Diversity, Structure, Volume 23, Issue 2, 2015, Pages 302-311, ISSN 0969-2126, https://doi.org/10.1016/j.str.2014.11.010. Attempts to explain how many CDR regions can be classifiable and why the H3 region does not have this same grouping, specifically focusing on the kink in the H3 region. They conclude that the H3 function is defined by the C terminal kink and that is why it is so important to the antibody. Explains the framework region has expected canonical conformations. — Preceding unsigned comment added by Immcarle70 (talk • contribs) 02:38, 31 January 2018 (UTC)