Translin is a DNA-binding protein that in humans is encoded by the TSN gene.[5][6][7] Together with translin-associated factor X, translin forms the component 3 of promoter of RISC (C3PO) complex which facilitates endonucleolytic cleavage of the passenger strand during microRNA loading into the RNA-induced silencing complex (RISC).[8][9][10]

TSN
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesTSN, BCLF-1, C3PO, RCHF1, REHF-1, TBRBP, TRSLN, translin
External IDsOMIM: 600575; MGI: 109263; HomoloGene: 3397; GeneCards: TSN; OMA:TSN - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001261401
NM_004622

NM_011650

RefSeq (protein)

NP_001248330
NP_004613

NP_035780

Location (UCSC)Chr 2: 121.74 – 121.77 MbChr 1: 118.22 – 118.24 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Function

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This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms.[7]

Interactions

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Translin has been shown to interact with PPP1R15A.[11]

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000211460Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026374Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Kasai M, Aoki K, Matsuo Y, Minowada J, Maziarz RT, Strominger JL (July 1994). "Recombination hotspot associated factors specifically recognize novel target sequences at the site of interchromosomal rearrangements in T-ALL patients with t(8;14)(q24;q11) and t(1;14)(p32;q11)". International Immunology. 6 (7): 1017–25. doi:10.1093/intimm/6.7.1017. PMID 7947454.
  6. ^ Aoki K, Inazawa J, Takahashi T, Nakahara K, Kasai M (July 1997). "Genomic structure and chromosomal localization of the gene encoding translin, a recombination hotspot binding protein". Genomics. 43 (2): 237–41. doi:10.1006/geno.1997.4796. PMID 9244443.
  7. ^ a b "Entrez Gene: TSN translin".
  8. ^ Liu Y, Ye X, Jiang F, Liang C, Chen D, Peng J, Kinch LN, Grishin NV, Liu Q (August 2009). "C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation". Science. 325 (5941): 750–3. Bibcode:2009Sci...325..750L. doi:10.1126/science.1176325. PMC 2855623. PMID 19661431.
  9. ^ Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H (June 2011). "Structure of C3PO and mechanism of human RISC activation". Nature Structural & Molecular Biology. 18 (6): 650–7. doi:10.1038/nsmb.2032. PMC 3109212. PMID 21552258.
  10. ^ Ha M, Kim VN (August 2014). "Regulation of microRNA biogenesis". Nature Reviews Molecular Cell Biology. 15 (8): 509–24. doi:10.1038/nrm3838. PMID 25027649. S2CID 205495632.
  11. ^ Hasegawa T, Isobe K (August 1999). "Evidence for the interaction between Translin and GADD34 in mammalian cells". Biochimica et Biophysica Acta (BBA) - General Subjects. 1428 (2–3): 161–8. doi:10.1016/s0304-4165(99)00060-4. PMID 10434033.

Further reading

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  • Overview of all the structural information available in the PDB for UniProt: Q15631 (Human Translin) at the PDBe-KB.
  • Overview of all the structural information available in the PDB for UniProt: Q62348 (Mouse Translin) at the PDBe-KB.