Sulfate reduction coupled to ammonium oxidation, or sulfammox, is a novel multi-step microbial process especially pertinent to industrial wastewater treatment. Microbial species associated with sulfammox include but are not limited to Anammoxoglobus sulfate, Bacillus benzoevorans, Candidatus_ Anammoxoglobus, Bacillus cereus SUD-1.[1][2][3][4] This list includes species that may perform sulfammox alongside other microbes, though SUD-1 was shown to perform sulfammox when isolated in an experiment.[5][4]

Application to wastewater treatment

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Wastewater from industrial activities presents a danger to human and aquatic life as present compounds may exacerbate the eutrophication of water bodies, leading to toxic algal blooms, and may poison aquatic organisms and, by extent, humans.[3] High levels of sulfate (SO42-) and ammonium (NH4+) are present in wastewater, contributing to its dangers and toxicity, and thus facilitating their removal via sulfammox may be important in protecting human and aquatic life. The microbial anaerobic oxidation of ammonium (anammox) has been cultivated at wastewater treatment center for years in order to remove NH4+, but the removal of SO42- has been typically treated as a separate problem requiring completely different microbial bioreactors – resulting in costly operations attempting to mitigate high SO42- and NH4+ separately.[3] The opportunity to unify both processes into a single system – utilizing sulfammox alongside anammox – may thus be appealing as a cost-efficient alternative to removing both SO42- and NH4+ from wastewater.

One study found that “after 180 days” of facilitating sulfammox and anammox in landfill leachates, “the nitrogen and sulfate removal efficiencies of 95.7% and 24.3%, respectively, were obtained” with sulfammox accounting for “27.5% of total inorganic nitrogen removal” and anammox accounting for “65.6%”.[6] Although sulfammox has been estimated to be less metabolically efficient than anammox, it also may consume less energy than other processes; sulfammox enhancement in a bioreactor saved “44%... in aeration energy consumption”.[3]

Another study observed that excessively high SO42- and NH4+ – “NH4+-N was 181.62 mg·(L·d)−1, SO42--S was 323.18 mg·(L·d)−1” – was toxic to sulfammox microbes and slowed the removal of the compounds from a bioreactor.[7] The study did reach similar removal efficiencies of NH4+ and SO42- as Zhang et al. 2023, with “94.80%” and “52.57%” removed respectively..[6] The experiment also involved the addition of bicarbonate which may have competed with SO42- to oxidize NH4+ and impeded the analysis of nitrogen and sulfur-compound removal rates [6] Artificial neural networks may assist in measuring the efficiency of sulfammox across various wastewater treatment centers and conditions, leading to its future optimization in removing harmful SO42- and NH4+ from industrial wastewater.[3]

History and possible redox background

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Sulfammox was first observed by Fdz-Polanco et al. 2001 in industrial wastewater treatment centers utilizing anaerobic bioreactors and it was largely believed to be exclusive to such artificial environments.[8] However, its presence has since been proposed and observed in natural anoxic environments – including marine sediments and anaerobic ocean waters [9] [10] [11]

Though the mechanisms of sulfammox are not entirely clear, multiple studies have attempted to model the process in a series of chemical reactions. According to Liu et al. 2008, the following system of chemical reactions may represent the sulfammox pathway without organic carbon: [1]

SO42- + NH4+ ←→ SO42- + NO2- + S + H2O (1)

NH4+ + NO2- ←→ N2 + 2H2O (2)

NH4+ + ½ SO42- ←→ ½ N2 + ½ S + 2H2O (3)

However, Bi et al. 2020 found that anammox microbes could not perform sulfammox alone under strict anaerobic conditions and that sulfammox was, rather, a combination of aerobic ammonium oxidation, anammox and heterotrophic sulfate reduction processes. Specifically, Bi et al. 2020 postulated that facultatively aerobic ammonia-oxidizing bacteria, found living alongside anammox microbes and protecting them from oxygen leakage, could accomplish Equation (1) in the above system of equations representing sulfammox and provide nitrite to anammox microbes. Subsequent sulfate reduction was found to be attributed to sulfur-reducing bacteria.[5]

Mohammed Madani et al. 2022, on the other hand, found that the SUD-1 strain of Bacillus cereus could perform sulfammox in isolation according to the model proposed by Liu et al. 2008. [1] [4] Liu et al. 2021 delineates sulfammox reactions involving organic carbon and sulfides: [12]

3SO42- + 8NH4+ ←→ 3HS- + 4N2 + 5H+ + 12H2O (4)

CH2O + 4NH4+ + 4SO42- ←→ 5CO2 + 2N2 + 4HS- + 11H2O (5)

Further models exist and multiple may partially or fully reflect present sulfammox processes both in artificial and natural environments.

Astrobiological ramifications

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Both Europa and Enceladus – Jupiter’s and Saturn’s moons respectively – may have a water subsurface ocean beneath their icy surface with present ammonia and sulfate.[13] The availability of these and other nutrients, including methane and potentially organics, have made them ideal candidates for plausible life outside of Earth. Considering certain sulfammox microbes’ preference for slightly alkaline environments (8), Enceladus’ alkaline subsurface ocean (8-11) may exhibit qualities more consistent with an environment capable of supporting sulfammox than Europa's potentially slightly acidic ocean.[3][13] Extraterrestrial life is completely hypothetical, however, and many generations of work remain until humans may be capable of investigating the plausibility of life on Europa and Enceladus.

References

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  1. ^ a b c Liu, Sitong; Yang, Fenglin; Gong, Zheng; Meng, Fangang; Chen, Huihui; Xue, Yuan; Furukawa, Kenji (October 2008). "Application of anaerobic ammonium-oxidizing consortium to achieve completely autotrophic ammonium and sulfate removal". Bioresource Technology. 99 (15): 6817–6825. Bibcode:2008BiTec..99.6817L. doi:10.1016/j.biortech.2008.01.054. PMID 18343660.
  2. ^ Cai, Jing; Jiang, JianXiang; Zheng, Ping (March 2010). "Isolation and identification of bacteria responsible for simultaneous anaerobic ammonium and sulfate removal". Science China Chemistry. 53 (3): 645–650. doi:10.1007/s11426-010-0053-8. S2CID 96010990.
  3. ^ a b c d e f Wu, Tong; Ding, Jie; Zhong, Le; Sun, Han-Jun; Pang, Ji-Wei; Zhao, Lei; Bai, Shun-Wen; Ren, Nan-Qi; Yang, Shan-Shan (October 2023). "Sulfate-reducing ammonium oxidation: A promising novel process for nitrogen and sulfur removal". Science of the Total Environment. 893: 164997. Bibcode:2023ScTEn.893p4997W. doi:10.1016/j.scitotenv.2023.164997. PMID 37336410. S2CID 259200898.
  4. ^ a b c Mohammed Madani, R., Liang, J., Cui, L. et al. “Novel Simultaneous Removal of Ammonium and Sulfate by Isolated Bacillus cereus Strain from Sewage Treatment Plant.” Water Air Soil Pollut 233, 185 (2022). https://doi.org/10.1007/s11270-022-05627-4
  5. ^ a b Bi, Zhen; Wanyan, Deqing; Li, Xiang; Huang, Yong (June 2020). "Biological conversion pathways of sulfate reduction ammonium oxidation in anammox consortia". Frontiers of Environmental Science & Engineering. 14 (3): 38. Bibcode:2020FrESE..14...38B. doi:10.1007/s11783-019-1217-1. S2CID 213559557.
  6. ^ a b c Zhang, Fangzhai; Ren, Shang; Liang, Haoran; Wang, Zhaozhi; Yan, Ying; Wang, Jiahui; Peng, Yongzhen (May 2023). "Efficient nitrogen removal and partial elemental sulfur recovery by combined nitrite-Anammox and sulfate-Anammox: A novel strategy for treating mature landfill leachate". Journal of Cleaner Production. 399: 136553. doi:10.1016/j.jclepro.2023.136553. S2CID 257266317.
  7. ^ Zhang, Dandan et al. “Treatment performance and microbial community under ammonium sulphate wastewater in a sulphate reducing ammonium oxidation process.” Environmental technology vol. 42,19 (2021): 2982-2990. doi:10.1080/09593330.2020.1720305
  8. ^ Fdz-Polanco, F et al. “New process for simultaneous removal of nitrogen and sulphur under anaerobic conditions.” Water research vol. 35,4 (2001): 1111-4. doi:10.1016/s0043-1354(00)00474-7
  9. ^ Schrum, H. et al. “Sulfate-reducing ammonium oxidation: A thermodynamically feasible metabolic pathway in subseafloor sediment.” Geology vol. 37,10 (2009): 939-942. doi:10.1130/G30238A.1
  10. ^ Rios-Del Toro, E Emilia et al. “Anaerobic ammonium oxidation linked to sulfate and ferric iron reduction fuels nitrogen loss in marine sediments.” Biodegradation vol. 29,5 (2018): 429-442. doi:10.1007/s10532-018-9839-8
  11. ^ Canfield, D. E. et al. “A Cryptic Sulfur Cycle in Oxygen-Minimum–Zone Waters off the Chilean Coast.” Science 330,1375-1378(2010). doi:10.1126/science.1196889
  12. ^ Liu, Lu-Yao et al. “Sulfate dependent ammonium oxidation: A microbial process linked nitrogen with sulfur cycle and potential application.” Environmental research vol. 192 (2021): 110282. doi:10.1016/j.envres.2020.110282
  13. ^ a b Weber, Jessica M.; Marlin, Theresa C.; Prakash, Medha; Teece, Bronwyn L.; Dzurilla, Katherine; Barge, Laura M. (11 August 2023). "A Review on Hypothesized Metabolic Pathways on Europa and Enceladus: Space-Flight Detection Considerations". Life. 13 (8): 1726. Bibcode:2023Life...13.1726W. doi:10.3390/life13081726. PMC 10456045. PMID 37629583.