Scotopic vision is the vision of the eye under low-light levels. The term comes from Greek skotos, meaning "darkness", and -opia, meaning "a condition of sight". In the human eye, cone cells are nonfunctional in low visible light. Scotopic vision is produced exclusively through rod cells, which are most sensitive to wavelengths of light around 498 nm (green–blue) and are insensitive to wavelengths longer than about 640 nm (red).
Scotopic vision is dominated by retina amacrine cells, specifically AII-amacrine cells. AII-amacrine cells capture rod bipolar cell input and redistribute it to cone bipolar cells since rod-driven bipolar cells do not synapse on ganglion cells. 
Scotopic vision occurs at luminance levels of 10−3 to 10−6 cd/m². Other species are not universally color blind in low-light conditions. The elephant hawk-moth (Deilephila elpenor) displays advanced color discrimination even in dim starlight.
Mesopic vision occurs in intermediate lighting conditions (luminance level 10−3 to 100.5 cd/m²) and is effectively a combination of scotopic and photopic vision. This gives inaccurate visual acuity and color discrimination.
The normal human observer’s relative wavelength sensitivity will not change due to background illumination change under scotopic vision. The wavelength sensitivity is determined by the rhodopsin photopigment. This is a red pigment seen at the back of the eye in animals that have a white background to their eye called Tapetum lucidum. The pigment is not noticeable under photopic and mesopic conditions. The principle that the wavelength sensitivity does not change during scotopic vision led to the ability to detect two functional cone classes in individuals. If two cone classes are present, then their relative sensitivity will change the behavioral wavelength sensitivity. Therefore, experimentation can determine “the presence of two cone classes by measuring wavelength sensitivity on two different backgrounds and noting a change in the observer’s relative wavelength sensitivity.” For adaption to occur at very low levels, the human eye needs to have a large sample of light across the signal in order to get a reliable image. This leads to the human eye being unable to resolve high spatial frequencies in low light since the observer is spatially averaging the light signal.
The behavior of the rhodopsin photopigment explains why the human eye cannot resolve lights with different spectral power distributions under low light. The reaction of this single photopigment will give the same quanta for 400 nm light and 700 nm light. Therefore, this photopigment only maps the rate of absorption and does not encode information about the relative spectral composition of the light.
Another reason that vision is poor under scotopic vision is that rods, which are the only cells active under scotopic vision, converge to a smaller number of neurons in the retina. This many-to-one ratio leads to poor spatial frequency sensitivity.
- "Scotopia". Dictionary.com.
- Marc, R. E.; Anderson, J. R.; Jones, B. W.; Sigulinsky, C. L.; Lauritzen, J. S. (2014). "The AII amacrine cell connectome: A dense network hub". Frontiers in Neural Circuits. 8: 104. doi:10.3389/fncir.2014.00104. PMC . PMID 25237297.
- Kelber, Almut; Balkenius, Anna; Warrant, Eric J. (31 October 2002). "Scotopic colour vision in nocturnal hawkmoths". Nature. 419 (6910): 922–925. doi:10.1038/nature01065 – via www.nature.com.
- Photobiology: The Science of Light and Life (2002), Lars Olof Björn, p.43, ISBN 1-4020-0842-2
- "Foundations of Vision". foundationsofvision.stanford.edu.