Leukocyte elastase inhibitor (LEI) also known as serpin B1 is a protein that in humans is encoded by the SERPINB1gene. It is a member of the clade B serpins or ov-serpins (ovalbumin related serpins) founded by ovalbumin.[5][6][7]
SerpinB1 is a cytoplasmic serine protease inhibitor of polymorphonuclear neutrophils. Among other serine proteases, it specifically inhibits neutrophil elastase, PR3 and cathepsin G, all found in neutrophil granules, by a suicide inhibition mechanism. SerpinB1 was found to reduce tissue damage caused by the mentioned proteases during inflammation and has a role in neutrophil homeostasis in mice. In various infection models (e.g. pneumonia) correlation of SerpinB1 absence and lack of microbial clearance have been shown. Different knockout strains serve as model to investigate the role of SerpinB1 in vivo.[8][9][10]
Rasmussen HH, van Damme J, Puype M, et al. (1993). "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes". Electrophoresis. 13 (12): 960–9. doi:10.1002/elps.11501301199. PMID1286667. S2CID41855774.
Packard BZ, Lee SS, Remold-O'Donnell E, Komoriya A (1995). "A serpin from human tumor cells with direct lymphoid immunomodulatory activity: mitogenic stimulation of human tumor-infiltrating lymphocytes". Biochim. Biophys. Acta. 1269 (1): 41–50. doi:10.1016/0167-4889(95)00113-7. PMID7578269.
Sugimori T, Cooley J, Hoidal JR, Remold-O'Donnell E (1995). "Inhibitory properties of recombinant human monocyte/neutrophil elastase inhibitor". Am. J. Respir. Cell Mol. Biol. 13 (3): 314–22. doi:10.1165/ajrcmb.13.3.7654387. PMID7654387.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID8125298.
Evans E, Cooley J, Remold-O'Donnell E (1996). "Characterization and chromosomal localization of ELANH2, the gene encoding human monocyte/neutrophil elastase inhibitor". Genomics. 28 (2): 235–40. doi:10.1006/geno.1995.1136. PMID8530031.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
Zeng W, Silverman GA, Remold-O'Donnell E (1998). "Structure and sequence of human M/NEI (monocyte/neutrophil elastase inhibitor), an Ov-serpin family gene". Gene. 213 (1–2): 179–87. doi:10.1016/S0378-1119(98)00189-9. PMID9630619.
Sun J, Stephens R, Mirza G, et al. (1999). "A serpin gene cluster on human chromosome 6p25 contains PI6, PI9 and ELANH2 which have a common structure almost identical to the 18q21 ovalbumin serpin genes". Cytogenet. Cell Genet. 82 (3–4): 273–7. doi:10.1159/000015118. PMID9858835. S2CID23659776.
Perani P, Zeggai S, Torriglia A, Courtois Y (2000). "Mutations on the hinge region of leukocyte elastase inhibitor determine the loss of inhibitory function". Biochem. Biophys. Res. Commun. 274 (3): 841–4. doi:10.1006/bbrc.2000.3191. PMID10924364.
Cooley J, Takayama TK, Shapiro SD, et al. (2002). "The serpin MNEI inhibits elastase-like and chymotrypsin-like serine proteases through efficient reactions at two active sites". Biochemistry. 40 (51): 15762–70. doi:10.1021/bi0113925. PMID11747453.
Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID12665801. S2CID23783563.
Ahmed M, Forsberg J, Bergsten P (2005). "Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry". J. Proteome Res. 4 (3): 931–40. doi:10.1021/pr050024a. PMID15952740.
