MLO may refer to:
- Malibu Locals Only, a group of youths local to Malibu, California, US
- Medio-lateral Oblique, a standard view used in mammography
- Milos Island National Airport, Greece, IATA code
- Mildew resistance locus o, a plant gene family
- Misanthropic Luciferian Order, a Satanic organisation
- Mortgage Loan Originator Housing and Economic Recovery Act of 2008 term for "loan officer"
- Mount Laguna Observatory, near San Diego, California, US
- Mycoplasma-like organism, earliest name for phytoplasmas
- Mauna Loa Observatory, an atmospheric baseline station near Hilo, Hawaii, US
- Multi-Link Operation, in Wi-Fi 7, a technology to increase capacity by bundling frequency bands and channels
This disambiguation page lists articles associated with the title MLO.
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