John Mayo may refer to:
- John Mayo (minister) (died 1676), Puritan minister in pre-revolutionary Boston, Massachusetts
- John Mayo (physician) (1761–1818), English doctor
- John Mayo (cricketer) (1822–1899), English cricketer
- John C. C. Mayo (1864–1914), American entrepreneur, educator and politician
- John S. Mayo (born 1930), American engineer
This disambiguation page lists articles about people with the same name.
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