Joe Palooka in Triple Cross
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After the champ, Joe Palooka, his wife Anne and trainer Knobby stop for gas, they pick up three hitchhikers who turn out to be fugitives from the law. Their leader is the Professor and his top henchman is Dutch, who is disguised as a woman.
While holding Ann hostage, the Professor orders Joe to lose his next fight on purpose while Knobby places a $100,000 bet on his foe. Dutch, carrying a gun, sits ringside, again dressed as a woman, to make sure Joe does what he's told.
Knocked out of the ring, Joe lands at Dutch's feet and exposes his true identity. While cops and security deal with that, Joe jumps back into the ring and flattens his opponent. He is winner and still champion.