In 1584 Guillemeau published Traité des maladies de l'oeil ("Treatise on eye diseases"), considered one of the best Renaissance-era works in ophthalmic medicine. He is also credited for providing the first description involving repair of palpebral coloboma, an eyelid defect he referred to as paupieres accurcies.
In 1609 he published De l'heureux accouchement des femmes ("The happy delivery of women"), the first description of a method of assisted breech delivery popularized by other physicians, and sometimes known as the "Mauriceau-Smellie-Veit maneuver". Guillemeau was a practitioner of the podalic version for use in cases of placenta praevia, a procedure earlier revived by Ambroise Paré.
Other publications by Guillemeau include Tables anatomiques and La chirurgie française.
- Poulain, François. La vie et l'œuvre de deux chirurgiens: Jacques Guillemeau et Charles Guillemeau. Montpellier. 1993 (in French)
- Radcliffe, Walter. Milestones in Midwifery, pioneers at the Hôtel-Dieu. Norman Publishing, 1947 ISBN 9780930405205, p. 23
- Speert, Harold. Obstetrics and gynecology: a history and iconography ISBN 9781842142783
- Online excerpts from Hunton's 1587 translation, A worthy treatise of the eyes
- Translated into English in 1612