Isoleucyl-tRNA synthetase, cytoplasmic is an enzyme that in humans is encoded by the IARS1 gene .[ 5] [ 6]
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs.[ 6]
^ a b c GRCh38: Ensembl release 89: ENSG00000196305 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000037851 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Nichols RC, Blinder J, Pai SI, Ge Q, Targoff IN, Plotz PH, Liu P (February 1997). "Assignment of two human autoantigen genes-isoleucyl-tRNA synthetase locates to 9q21 and lysyl-tRNA synthetase locates to 16q23-q24" . Genomics . 36 (1): 210–3. doi :10.1006/geno.1996.0449 . PMID 8812440 . ^ a b "Entrez Gene: IARS1 isoleucyl-tRNA synthetase" .^ Rho, S B; Lee J S; Jeong E J; Kim K S; Kim Y G; Kim S (May 1998). "A multifunctional repeated motif is present in human bifunctional tRNA synthetase" . J. Biol. Chem . 273 (18). UNITED STATES: 11267–73. doi :10.1074/jbc.273.18.11267 ISSN 0021-9258 . PMID 9556618 .
Norcum MT (1991). "Structural analysis of the high molecular mass aminoacyl-tRNA synthetase complex. Effects of neutral salts and detergents" . J. Biol. Chem . 266 (23): 15398–405. doi :10.1016/S0021-9258(18)98629-1 PMID 1651330 . Nichols RC, Raben N, Boerkoel CF, Plotz PH (1995). "Human isoleucyl-tRNA synthetase: sequence of the cDNA, alternative mRNA splicing, and the characteristics of an unusually long C-terminal extension" . Gene . 155 (2): 299–304. doi :10.1016/0378-1119(94)00634-5 . PMID 7721108 . Shiba K, Suzuki N, Shigesada K, et al. (1994). "Human cytoplasmic isoleucyl-tRNA synthetase: selective divergence of the anticodon-binding domain and acquisition of a new structural unit" . Proc. Natl. Acad. Sci. U.S.A . 91 (16): 7435–9. Bibcode :1994PNAS...91.7435S . doi :10.1073/pnas.91.16.7435 PMC 44415 PMID 8052601 . Rho SB, Lee KH, Kim JW, et al. (1996). "Interaction between human tRNA synthetases involves repeated sequence elements" . Proc. Natl. Acad. Sci. U.S.A . 93 (19): 10128–33. Bibcode :1996PNAS...9310128R . doi :10.1073/pnas.93.19.10128 PMC 38348 PMID 8816763 . Degoul F, Brulé H, Cepanec C, et al. (1998). "Isoleucylation properties of native human mitochondrial tRNAIle and tRNAIle transcripts. Implications for cardiomyopathy-related point mutations (4269, 4317) in the tRNAIle gene" . Hum. Mol. Genet . 7 (3): 347–54. doi :10.1093/hmg/7.3.347 PMID 9466989 . Rho SB, Lee JS, Jeong EJ, et al. (1998). "A multifunctional repeated motif is present in human bifunctional tRNA synthetase" . J. Biol. Chem . 273 (18): 11267–73. doi :10.1074/jbc.273.18.11267 PMID 9556618 . Quevillon S, Robinson JC, Berthonneau E, et al. (1999). "Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein". J. Mol. Biol . 285 (1): 183–95. doi :10.1006/jmbi.1998.2316 . PMID 9878398 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Bouwmeester T, Bauch A, Ruffner H, et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol . 6 (2): 97–105. doi :10.1038/ncb1086 . PMID 14743216 . S2CID 11683986 . Humphray SJ, Oliver K, Hunt AR, et al. (2004). "DNA sequence and analysis of human chromosome 9" . Nature . 429 (6990): 369–74. Bibcode :2004Natur.429..369H . doi :10.1038/nature02465 . PMC 2734081 PMID 15164053 . Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes" . Genome Res . 16 (1): 55–65. doi :10.1101/gr.4039406 . PMC 1356129 PMID 16344560 . Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry" . Mol. Syst. Biol . 3 (1): 89. doi :10.1038/msb4100134 . PMC 1847948 PMID 17353931 .
