Hiss is a surname. Notable people with the surname include:

  • Alger Hiss (1904–1996), U.S. State Department employee and an accused Soviet spy
  • Anna Hiss (1893–1979), 20th-Century American professor of physical education
  • August Hiss, Swiss footballer
  • Arlene Hiss (born 1941), American former race car driver and schoolteacher
  • Donald Hiss (1906–1989), younger brother of Alger Hiss
  • Mike Hiss (1941–2018), former driver in the USAC Championship Car series
  • Priscilla Hiss (1903–1984), a 20th-century American teacher and book editor
  • Yehuda Hiss (born 1946), chief pathologist at the Abu Kabir Institute of Forensic Medicine