He was born in Sheffield, the son of a mechanical engineer, and educated at Repton School and Corpus Christi, Cambridge. He studied medicine at the Middlesex Hospital Medical School and was awarded MB BChir in 1948.
From 1940 to 1947 he served with the Royal Army Medical Corps, retiring as a lieutenant-colonel. He was appointed an OBE for his military work in the provision of blood for transfusions throughout Europe and the Middle East. On his return to Britain he was appointed to the post of founding director of the Ciba Foundation (now Novartis Foundation), an organisation established to encourage international co-operation in scientific research.
He was president of the Royal Society of Medicine from 1975 to 1977, Harveian Librarian of the Royal College of Physicians from 1979 to 1989 and Master of the Worshipful Society of Apothecaries from 1979 to 1980. He was knighted in 1976.
He died in 2004 and was survived by his second wife Dushanka.
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- "Munks Roll Details for Sir Gordon Ethelbert Ward Wolstenholme". Munksroll.rcplondon.ac.uk. Retrieved 2013-09-03.
- Bynuma1, W. F. "Cambridge Journals Online - Medical History - Abstract - Sir Gordon Wolstenholme (editor), Lives of the Fellows of the Royal College of Physicians (Munk's Roll), Oxford and Washington DC, IRL Press; vol. 6: 1966-75, 1982, 8vo, pp. xi, 505, illus., £25.00; vol. 7: 1976-83, 1984, 8vo, pp. xi, 646, illus., £35.00". Journals.cambridge.org. Retrieved 2013-09-03.
- "Sir Gordon Wolstenholme". Daily Telegraph. Retrieved 17 May 2015.
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