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Summary
| Description |
English: 3D-animation of a Diatom (Corethron sp.) which illustrates how the e-HCFM method supports investigation about microbial interactions The specimen (surface water, Tara Ocean station 137) was imaged manually from regular e-HCFM sample preparation with a Leica SP8 confocal laser scanning microscope (40X NA1.1 water). Four fluorescent channels are recorded: (i) Green: cellular membranes (DiOC6(3)) indicate the core cell bodies; (ii) Blue: DNA (Hoechst) is used to identify the nucleus; (iii) Red: chlorophyll (autofluorescence); (iv) Grey: PLL-Alexa546 is a useful counterstain for visualizing the specimen surface. 3D reconstruction, surface rendering and animation were conducted with the software Imaris (Bitplane).
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| Date | |
| Source | doi:10.7554/eLife.26066.018 |
| Author | Sebastien Colin, Luis Pedro Coelho, Shinichi Sunagawa, Chris Bowler, Eric Karsenti, Peer Bork, Rainer Pepperkok, Colomban de Vargas |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 12:59, 5 October 2020 | 20 s, 1,240 × 720 (11.7 MB) | Epipelagic | Uploaded a work by Sebastien Colin, Luis Pedro Coelho, Shinichi Sunagawa, Chris Bowler, Eric Karsenti, Peer Bork, Rainer Pepperkok, Colomban de Vargas from [https://elifesciences.org/articles/26066#fig2video1] {{doi|10.7554/eLife.26066.018}} with UploadWizard |
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