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Arthrospira

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Arthrospira is a genus of free-floating filamentous cyanobacteria characterized by cylindrical, multicellular trichomes in an open left-hand helix. A dietary supplement is made from A. platensis and A. maxima, known as spirulina.[1] The A. maxima and A. platensis species were once classified in the genus Spirulina. Although the introduction of the two separate genera Arthrospira and Spirulina is now generally accepted, there has been much dispute in the past and the resulting taxonomical confusion is tremendous.[2]

Arthrospira
A single Arthrospira platensis colony
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Cyanobacteria
Class: Cyanophyceae
Order: Oscillatoriales
Family: Microcoleaceae
Genus: Arthrospira
Sitzenberger ex Gomont, 1892
Species

About 35.

Spirulina powder, from the genus Arthrospira, on unstained wet mount under 400x magnification

Taxonomy

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The common name, spirulina, refers to the dried biomass of Arthrospira platensis,[3] a type of Cyanobacteria, which are oxygenic photosynthetic bacteria. These photosynthetic organisms were first considered to be algae, a very large and diverse group of eukaryotic organisms, until 1962 when they were reclassified as prokaryotes and named Cyanobacteria.[4] This designation was accepted and published in 1974 by Bergey's Manual of Determinative Bacteriology.[5] Scientifically, quite a distinction exists between the Spirulina and Arthrospira genera. Stizenberger, in 1852, gave the name Arthrospira based on the presence of septa, its helical form, and its multicellular structure, and Gomont, in 1892, confirmed the aseptate form of the genus Spirulina. Geitler in 1932 reunified both members designating them as Spirulina without considering the septum.[6] Research on microalgae was carried out in the name of Spirulina, but the original species used to produce the dietary supplement spirulina belongs to the genus Arthrospira. This misnomer has been difficult to correct.[5]

At present, taxonomy states that the name spirulina for strains which are used as food supplements is inappropriate, and agreement exists that Arthrospira is a distinct genus, consisting of over 30 different species, including A. platensis and A. maxima.[7] A 2019 analysis of Arthrospira species using 16S rRNA gene sequence suggests that certain species of this genus (A. jenneri) is much closer to Planktothrix clade than previously thought. It also lacks characteristics of mass produced species (such as preference of alkaline habitats). As a result, researchers proposed a new genus closer to Limnoraphis and Neolyngbya called Limnospira comprising L. fusiformis, L. maxima and L. indica.[8]

Morphology

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The genus Arthrospira comprises helical trichomes of varying size and with various degrees of coiling, including tightly-coiled morphology to a straight form.[1]

The helical parameters of the shape of Arthrospira is used to differentiate between and even within the same species.[9][10] These differences may be induced by changing environmental conditions, such as temperature.[11] The helical shape of the trichomes is only maintained in a liquid environment.[12] The filaments are solitary and reproduce by binary fission, and the cells of the trichomes vary in length from 2 to 12 μm and can sometimes reach 16 μm.

Biochemical composition

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Further information: Spirulina (dietary supplement)

Arthrospira is very rich in proteins,[1][12] and constitute 53 to 68 percent by dry weight of the contents of the cell.[13] Its protein harbours all essential amino acids.[12] Arthrospira also contain high amounts of polyunsaturated fatty acids (PUFAs), about 1.5–2 percent, and a total lipid content of 5–6 percent.[12] These PUFAs contain the γ-linolenic acid (GLA), an omega-6 fatty acid.[14] Further contents of Arthrospira include vitamins, minerals and photosynthetic pigments.[12]

Occurrence

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Species of the genus Arthrospira have been isolated from alkaline brackish and saline waters in tropical and subtropical regions. Among the various species included in the genus, A. platensis is the most widely distributed and is mainly found in Africa, but also in Asia. A. maxima is believed to be found in California and Mexico.[6] A. platensis and A. maxima occur naturally in tropical and subtropical lakes with alkaline pH and high concentrations of carbonate and bicarbonate.[12] A. platensis occurs in Africa, Asia and South America, whereas A. maxima is confined to Central America. A. pacifica is endemic to the Hawaiian islands.[15] Most cultivated spirulina is produced in open-channel raceway ponds, with paddle-wheels used to agitate the water.[12] The largest commercial producers of spirulina are located in the United States, Thailand, India, Taiwan, China, Pakistan, Myanmar, Greece and Chile.[15]

Present and future uses

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Spirulina is widely known as a food supplement, but there are other possible uses for this cyanobacterium. As an example, it is suggested to be used medically for patients for whom it is difficult to chew or swallow food, or as a natural and cheap drug delivery system.[16] Further, promising results in the treatment of certain cancers, allergies and anemia, as well as hepatotoxicity and vascular diseases were found.[17] Spirulina may also be used as a healthy addition to animal feed[18] if the price of its production can be further reduced. Spirulina can be used in technical applications, such as the biosynthesis of silver nanoparticles, which allows the formation of metallic silver in an environmentally friendly way.[19] In the creation of textiles it harbors some advantages, since it can be used for the production of antimicrobial textiles[20] and paper or polymer materials.[20] They also may have an antioxidant effect[21] and may maintain the ecological balance in aquatic bodies and reduces various stresses in the aquatic environment.[22]

Cropping systems

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Growth of A. platensis depends on several factors. To achieve maximum output, factors such as the temperature, light and photoinhibition, nutrients and carbon dioxide level, need to be adjusted. In summer the main limiting factor of spirulina growth is light. When growing in water depths of 12–15 cm, self-shading governs the growth of the individual cell. However, research has shown, that growth is also photoinhibited, and can be increased through shading.[23] The level of photoinhibition versus the lack of light is always a question of cell concentration in the medium. The optimal growth temperature for A. platensis is 35–38 °C. This poses a major limiting factor outside the tropics, confining growth to the summer months.[24] A. platensis has been grown in fresh water, as well as in brackish water and sea water.[25] Apart from mineral fertilizer, various sources such as waste effluents, and effluents from fertilizer, starch and noodle factories have been used as a nutrient source.[15] Waste effluents are more readily available in rural locations, allowing small scale production.[26] One of the major hurdles for large scale production is the complicated harvesting process which accounts for 20–30% of the total production costs. Due to their small cell size, and diluted cultures (mass concentration less than 1 g/L) with densities close to that of water microalgae, they are difficult to separate from their growing medium.[27]

Cultivation systems

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Main article: Culture of microalgae in hatcheries

Open pond

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Open pond systems are the most common way to grow A. platensis due to their comparatively low cost. Typically, channels are built in form of a raceway from concrete or PVC coated earth walls, and water is moved by paddle wheels. The open design, however allows contamination by foreign algae and/or microorganisms.[15] Another problem includes water loss due to evaporation. Both of these problems can be addressed by covering the channels with transparent polyethylene film.[5]

Closed system

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Closed systems have the advantage of being able to control the physical, chemical and biological environment. This allows for increased yield, and more control of the nutrient level. Typical forms such as tubes or polyethylene bags, also offer a larger surface-to-volume ratios than open pond systems,[28] thus increasing the amount of sunlight available for photosynthesis. These closed systems help expanding the growing period into the winter months, but often lead to overheating in summer.[29]

Market potentials and feasibility

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Cultivation of Arthrospira has occurred for a long period of time,[vague] especially in Mexico and around Lake Chad on the African continent. During the 21st century however, its beneficial properties were rediscovered and therefore studies about Arthrospira and its production increased.[12] In the past decades, large-scale production of the cyanobacterium developed.[30] Japan started in 1960, and in the following years Mexico and several other countries over all continents, such as China, India, Thailand, Myanmar and the United States started to produce on large-scale.[12] In little time, China has become the largest producer worldwide.[30] A particular advantage of the production and use of spirulina is that its production can be conducted at a number of different scales, from household culture to intensive commercial production over large areas.

Especially as a small-scale crop, Arthrospira still has considerable potential for development, for example for nutritional improvement.[31] New countries where this could happen, should dispose of alkaline-rich ponds on high altitudes or saline-alkaline-rich groundwater or coastal areas with high temperature.[12] Otherwise, technical inputs needed for new spirulina farms are quite basic.[31]

The international market of spirulina is divided into two target groups: the one includes NGO’s and institutions focusing on malnutrition and the other includes health conscious people. There are still some countries, especially in Africa, that produce at a local level. Those could respond to the international demand by increasing production and economies of scale. Growing the product in Africa could offer an advantage in price, due to low costs of labour. On the other hand, African countries would have to surpass quality standards from importing countries, which could again result in higher costs.[31]

References

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  1. ^ a b c Ciferri, O. (1983). "Spirulina, the edible microorganism". Microbiological Reviews. 47 (4): 551–578. doi:10.1128/MMBR.47.4.551-578.1983. PMC 283708. PMID 6420655.
  2. ^ Mühling, Martin (March 2000). Characterization of Arthrospira (Spirulina) Strains (Ph.D.). University of Durham. Archived (PDF) from the original on 2016-01-23. Retrieved 2016-01-23.
  3. ^ Gershwin, ME; Belay, A (2007). Spirulina in human nutrition and health. CRC Press, USA.
  4. ^ Stanier, RY; Van Niel, Y (January 1962). "The concept of a bacterium". Archiv für Mikrobiologie. 42: 17–35. doi:10.1007/bf00425185. PMID 13916221. S2CID 29859498.
  5. ^ a b c Sánchez, Bernal-Castillo; Van Niel, J; Rozo, C; Rodríguez, I (2003). "Spirulina (Arthrospira): an edible microorganism: a review". Universitas Scientiarum. 8 (1): 7–24.
  6. ^ a b Siva Kiran, RR; Madhu, GM; Satyanarayana, SV (2016). "Spirulina in combating Protein Energy Malnutrition (PEM) and Protein Energy Wasting (PEW) - A review". Journal of Nutrition Research. 3 (1): 62–79. doi:10.55289/jnutres/v3i1.5.
  7. ^ Takatomo Fujisawa; Rei Narikawa; Shinobu Okamoto; Shigeki Ehira; Hidehisa Yoshimura; Iwane Suzuki; Tatsuru Masuda; Mari Mochimaru; Shinichi Takaichi; Koichiro Awai; Mitsuo Sekine; Hiroshi Horikawa; Isao Yashiro; Seiha Omata; Hiromi Takarada; Yoko Katano; Hiroki Kosugi; Satoshi Tanikawa; Kazuko Ohmori; Naoki Sato; Masahiko Ikeuchi; Nobuyuki Fujita & Masayuki Ohmori (2010-03-04). "Genomic Structure of an Economically Important Cyanobacterium, Arthrospira (Spirulina) platensis NIES-39". DNA Research. 17 (2): 85–103. doi:10.1093/dnares/dsq004. PMC 2853384. PMID 20203057. In its turn, it references: Castenholz R.W.; Rippka R.; Herdman M.; Wilmotte A. (2007). Boone D.R.; Castenholz R.W.; Garrity G.M. (eds.). Bergey's Manual of Systematic Bacteriology (2nd ed.). Springer: Berlin. pp. 542–3.
  8. ^ Nowicka-Krawczyk, Paulina; Mühlsteinová, Radka; Hauer, Tomáš (2019-01-24). "Detailed characterization of the Arthrospira type species separating commercially grown taxa into the new genus Limnospira (Cyanobacteria)". Scientific Reports. 9 (1): 694. doi:10.1038/s41598-018-36831-0. ISSN 2045-2322. PMC 6345927. PMID 30679537.
  9. ^ Rich, F (1931). "Notes on Arthrospira platensis". Revue Algologique. 6: 75–79.
  10. ^ Marty, F; Busson, F (1970). "Données cytologiques sur deux Cyanophycées: Spirulina platensis (Gom.) Geitler et Spirulina geitleri J. de Toni". Schweizerische Zeitschritf für Hydrologie. 32 (2): 559–565. doi:10.1007/bf02502570. S2CID 44855904.
  11. ^ Van Eykelenburg, C (1977). "On the morphology and ultrastructure of the cell wall of Spirulina platensis". Antonie van Leeuwenhoek. 43 (2): 89–99. doi:10.1007/bf00395664. PMID 413479. S2CID 22249310.
  12. ^ a b c d e f g h i j Habib, M. Ahsan B.; Parvin, Mashuda; Huntington, Tim C.; Hasan, Mohammad R. (2008). "A Review on Culture, Production and Use of Spirulina as Food dor Humans and Feeds for Domestic Animals and Fish" (PDF). Food and Agriculture Organization of The United Nations. Retrieved November 20, 2011.
  13. ^ Phang, S. M. (2000). "Spirulina cultivation in digested sago starch factory wastewater". Journal of Applied Phycology. 12 (3/5): 395–400. doi:10.1023/A:1008157731731. S2CID 20718419.
  14. ^ Spolaore, Pauline; et al. (2006). "Commercial applications of microalgae". Journal of Bioscience and Bioengineering. 101 (2): 87–96. doi:10.1263/jbb.101.87. PMID 16569602. S2CID 16896655.
  15. ^ a b c d Vonshak, Avigad (2002). Spirulina platensis (Arthrospira): Physiology, Cell-Biology And Biotechnology. CRC Press. ISBN 9780203483961.
  16. ^ Adiba, B. D.; et al. (2008). "Preliminary characterization of food tablets from date (Phoenix dactylifera L.) and spirulina (Spirulina sp.) powders". Powder Technology. 208 (3): 725–730. doi:10.1016/j.powtec.2011.01.016.
  17. ^ Asghari, A.; et al. (2016). "A Review on Antioxidant Properties of Spirulin". Journal of Applied Biotechnology Reports.
  18. ^ Holman, B. W. B.; et al. (2012). "Spirulina as a livestock supplement and animal feed". Journal of Animal Physiology and Animal Nutrition. 97 (4): 615–623. doi:10.1111/j.1439-0396.2012.01328.x. PMID 22860698.
  19. ^ Mahdieh (2012). "Green biosynthesis of silver nanoparticles by Spirulina platensis". Scientia Iranica. 19 (3): 926–929. doi:10.1016/j.scient.2012.01.010.
  20. ^ a b Mahltig, B; et al. (2013). "Modification of algae with zinc, copper and silver ions for usage as natural composite for antibacterial applications". Materials Science and Engineering. 33 (2): 979–983. doi:10.1016/j.msec.2012.11.033. PMID 25427514.
  21. ^ Kumaresan, Venkatesh; Sannasimuthu, Anbazahan; Arasu, Mariadhas Valan; Al-Dhabi, Naif Abdullah; Arockiaraj, Jesu (2018). "Molecular insight into the metabolic activities of a protein-rich micro alga, Arthrospira platensis by de novo transcriptome analysis". Molecular Biology Reports. 45 (5): 829–838. doi:10.1007/s11033-018-4229-1. PMID 29978380. S2CID 254835532.
  22. ^ Kumaresan, Venkatesh; Nizam, Faizal; Ravichandran, Gayathri; Viswanathan, Kasi; Palanisamy, Rajesh; Bhatt, Prasanth; Arasu, Mariadhas Valan; Al-Dhabi, Naif Abdullah; Mala, Kanchana; Arockiaraj, Jesu (2017). "Transcriptome changes of blue-green algae, Arthrospira sp. in response to sulfate stress". Algal Research. 23: 96–103. doi:10.1016/j.algal.2017.01.012.
  23. ^ Vonshak, A; Guy, R (1988). Photoinhibition as a limiting factor in outdoor cultivation of Spirulina platensis. In Stadler et al. eds. Algal Biotechnology. London: Elsevier Applied Sci. Publishers.
  24. ^ Vonshak, A (1997). Spirulina platensis (Arthrospira). In Physiology, Cell Biology and Biotechnology. Basingstoke, Hants, London: Taylor and Francis.
  25. ^ Materassi, R; et al. (1984). "Spirulina culture in sea-water". Applied Microbiology and Biotechnology. 19 (6): 384–386. doi:10.1007/bf00454374. S2CID 31267876.
  26. ^ Laliberte, G; et al. (1997). Mass cultivation and wastewater treatment using Spirulina. In A. Vonshak, ed. Spirulina platensis (Arthrospira platensis) Physiology, Cell Biology and Biotechnology. Basingstoke, Hants, London: Taylor and Francis. pp. 159–174.
  27. ^ Barros, Ana I.; et al. (2015). "Harvesting techniques applied to microalgae: A review". Renewable and Sustainable Energy Reviews. 41: 1489–1500. doi:10.1016/j.rser.2014.09.037. hdl:10216/103426.
  28. ^ Tredici, M; Materassi, R (1992). "From open ponds to vertical alveolar panels: the Italian experience in the development of reactors for the mass cultivation of phototrophic microorganisms". Journal of Applied Phycology. 4 (3): 221–231. doi:10.1007/bf02161208. S2CID 20554506.
  29. ^ Tomaselli, L; et al. (1987). "Recent research on Spirulina in Italy". Hydrobiology. 151/152: 79–82. doi:10.1007/bf00046110. S2CID 9903582.
  30. ^ a b Whitton, B. A. (2012). Ecology of Cyanobacteria II: Their Diversity in Space and Time. Springer. pp. 701–711.
  31. ^ a b c Smart Fish (2011). "Spirulina – a livelihood and a business venture". Report: SF/2011.
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  • Guiry, M.D.; Guiry, G.M. "Arthrospira". AlgaeBase. World-wide electronic publication, National University of Ireland, Galway.
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