DBO may refer to:
- Database owner (SQL), which can refer to either the physical owner, a user role, or a schema
- Dragon Ball Online
- D-Bo (born 1978), German rapper
- Daulat Beg Oldi
- DBO Energy, an independent Brazilian Oil & Gas company, focused on mature, producing fields
- DB Oil Fund, run by Invesco PowerShares
- Dubbo City Regional Airport
- Dead Blackout (Theatre Lighting)
- The unit dBO, decibels below overload
- Design, build and operate, a type of contract for project delivery and operation